Yeast cell wall and its entire contents disruption treatments are required in the enhancement of protein and the overall biodegradability of the cell wall materials during homogenization process. Yeast as a cheap, good resource and easily available source of energy from biomass into biogas, it was used as a substrate for the cleaner energy production due to its richly and high level content of protein contained in it. An initial study on the effects of high-pressure homogenizer mechanical pretreatment has been conducted in sequence as generated by the design matrix of the design of experiment (DOE) focusing on protein yields from bakers’ yeast also known as Saccharomyces cerevisiae and in order to achieve the maximum yield of protein which in other words aid biogas production, the following optimum process parameters were set in. The yeast block was refrigerated at between 0 – 4°C with fermentation at (0 – 24h), a pH value of (5.3) maximum was used in the preparation of the buffer solution C. This was obtained through diluting solution B into A until the pH was attained (details as shown in the materials and methods section). Number of cycles (passes) of the soluble yeast were undergone to enable the yeast cell walls be broken down for the release of more protein and at temperature range (15 – 25°C). The pressure for the compressed state during homogenization was set between (30 – 90MPa). The results presented therefore showed the rates of protein released from the disruption through using the Design Expert Software V.8 in identifying the ideal conditions as set in the parameters.
- Design of Experiment
- Cleaner Energy
Ekpeni, L. E. N., Nkem-Ekpeni, F. F., Benyounis, K. Y., Aboderheeba, A. K. M., Stokes, J., & Olabi, A. G. (2014). Yeast: a potential biomass substrate for the production of cleaner energy (biogas). Energy Procedia, 61, 1718-1731. https://doi.org/10.1016/j.egypro.2014.12.199