Utilising polyphenols for the clinical management of Candida albicans biofilms

Muhammad Shahzad, Leighann Sherry, Ranjith Rajendran, Christine A. Edwards, Emilie Combet, Gordon Ramage

Research output: Contribution to journalArticle

Abstract

Polyphenols (PPs) are secondary metabolites abundant in plant-derived foods. They are reported to exhibit antimicrobial activity that may offer an alternative to existing antimicrobials. The aim of this study was to evaluate the antifungal potential of PPs against Candida albicans biofilms that are commonly recalcitrant to antifungal therapy. The antifungal activity of 14 PPs was assessed in terms of planktonic and sessile minimum inhibitory concentrations (PMICs and SMICs, respectively) against various C. albicans clinical isolates. The most active PPs were further tested for their effect on C. albicans adhesion and biofilm growth using standard biomass assays, microscopy and quantitative gene expression. Of the 14 PPs tested, 7 were effective inhibitors of planktonic growth, of which pyrogallol (PYG) was the most effective (PMIC₅₀=78 μg/mL), followed by curcumin (CUR) (PMIC₅₀=100 μg/mL) and pyrocatechol (PMIC₅₀=625 μg/mL). Both PYG and CUR displayed activity against C. albicans biofilms (SMIC₅₀=40 μg/mL and 50 μg/mL, respectively), although they did not disrupt the biofilm or directly affect the cellular structure. Overall, CUR displayed superior biofilm activity, significantly inhibiting initial cell adhesion following pre-coating (P<0.01), biofilm growth (P<0.05) and gene expression (P<0.05). This inhibitory effect diminished with prolonged CUR exposure, although it still inhibited by 50% after 4h adhesion. Overall, CUR exhibited positive antibiofilm properties that could be used at the basis for development of similar molecules, although further cellular and in vivo studies are required to explore its precise mechanism of action.
Original languageEnglish
Pages (from-to)269-273
Number of pages5
JournalInternational journal of antimicrobial agents
Volume44
Issue number3
DOIs
Publication statusE-pub ahead of print - 6 Jul 2014
Externally publishedYes

Fingerprint

Polyphenols
Biofilms
Curcumin
Candida albicans
Pyrogallol
Gene Expression
Growth Inhibitors
Edible Plants
Microbial Sensitivity Tests
Cellular Structures
Growth
Cell Adhesion
Biomass
Microscopy

Cite this

Shahzad, Muhammad ; Sherry, Leighann ; Rajendran, Ranjith ; Edwards, Christine A. ; Combet, Emilie ; Ramage, Gordon. / Utilising polyphenols for the clinical management of Candida albicans biofilms. In: International journal of antimicrobial agents. 2014 ; Vol. 44, No. 3. pp. 269-273.
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Utilising polyphenols for the clinical management of Candida albicans biofilms. / Shahzad, Muhammad; Sherry, Leighann; Rajendran, Ranjith; Edwards, Christine A.; Combet, Emilie; Ramage, Gordon.

In: International journal of antimicrobial agents, Vol. 44, No. 3, 06.07.2014, p. 269-273.

Research output: Contribution to journalArticle

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T1 - Utilising polyphenols for the clinical management of Candida albicans biofilms

AU - Shahzad, Muhammad

AU - Sherry, Leighann

AU - Rajendran, Ranjith

AU - Edwards, Christine A.

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AU - Ramage, Gordon

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