The toxoplasma gondii plastid replication and repair enzyme complex, PREX

A. Mukhopadhyay, C-Y. Chen, C. Doerig, F. L. Henriquez-Mui, C. W. Roberts, M. P. Barrett

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A plastid-like organelle, the apicoplast, is essential to the majority of medically and veterinary important apicomplexan protozoa including Toxoplasma gondii and Plasmodium. The apicoplast contains multiple copies of a 35 kb genome, the replication of which is dependent upon nuclear-encoded proteins that are imported into the organelle, In P. falciparum an unusual multi-functional gene, pfprex, was previously identified and inferred to encode a protein with DNA primase, DNA helicase and DNA polymerase activities. Herein, we report the presence of a prex orthologue in T. gondii. The protein is predicted to have a bi-partite apicoplast targeting sequence similar to that demonstrated on the PfPREX polypeptide, capable of delivering marker proteins to the apicoplast. Unlike the P. falciparum gene that is devoid of introns, the T. gondii prex gene carries 19 introns, which are spliced to produce a contiguous mRNA. Bacterial expression of the polymerase domain reveals the protein to be active. Consistent with the reported absence of a plastid in Cryptosporidium species, in silico analysis of their genomes failed to demonstrate an orthologue of prex. These studies indicate that prex is conserved across the plastid-bearing apicomplexans and may play an important role in the replication of the plastid genome.
Original languageEnglish
Pages (from-to)747-755
Number of pages9
JournalParasitology
Volume136
Issue number7
DOIs
Publication statusPublished - Jun 2009
Externally publishedYes

Keywords

  • Apicomplexa
  • DNA replication
  • Plasmodium falciparum
  • Toxoplasma gondii
  • Exon
  • Intron
  • DNA polymerase
  • DNA primase
  • DNA helicase

Cite this

Mukhopadhyay, A., Chen, C-Y., Doerig, C., Henriquez-Mui, F. L., Roberts, C. W., & Barrett, M. P. (2009). The toxoplasma gondii plastid replication and repair enzyme complex, PREX. Parasitology, 136(7), 747-755. https://doi.org/10.1017/S0031182009006027
Mukhopadhyay, A. ; Chen, C-Y. ; Doerig, C. ; Henriquez-Mui, F. L. ; Roberts, C. W. ; Barrett, M. P. / The toxoplasma gondii plastid replication and repair enzyme complex, PREX. In: Parasitology. 2009 ; Vol. 136, No. 7. pp. 747-755.
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Mukhopadhyay, A, Chen, C-Y, Doerig, C, Henriquez-Mui, FL, Roberts, CW & Barrett, MP 2009, 'The toxoplasma gondii plastid replication and repair enzyme complex, PREX', Parasitology, vol. 136, no. 7, pp. 747-755. https://doi.org/10.1017/S0031182009006027

The toxoplasma gondii plastid replication and repair enzyme complex, PREX. / Mukhopadhyay, A.; Chen, C-Y.; Doerig, C.; Henriquez-Mui, F. L.; Roberts, C. W.; Barrett, M. P.

In: Parasitology, Vol. 136, No. 7, 06.2009, p. 747-755.

Research output: Contribution to journalArticle

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T1 - The toxoplasma gondii plastid replication and repair enzyme complex, PREX

AU - Mukhopadhyay, A.

AU - Chen, C-Y.

AU - Doerig, C.

AU - Henriquez-Mui, F. L.

AU - Roberts, C. W.

AU - Barrett, M. P.

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N2 - A plastid-like organelle, the apicoplast, is essential to the majority of medically and veterinary important apicomplexan protozoa including Toxoplasma gondii and Plasmodium. The apicoplast contains multiple copies of a 35 kb genome, the replication of which is dependent upon nuclear-encoded proteins that are imported into the organelle, In P. falciparum an unusual multi-functional gene, pfprex, was previously identified and inferred to encode a protein with DNA primase, DNA helicase and DNA polymerase activities. Herein, we report the presence of a prex orthologue in T. gondii. The protein is predicted to have a bi-partite apicoplast targeting sequence similar to that demonstrated on the PfPREX polypeptide, capable of delivering marker proteins to the apicoplast. Unlike the P. falciparum gene that is devoid of introns, the T. gondii prex gene carries 19 introns, which are spliced to produce a contiguous mRNA. Bacterial expression of the polymerase domain reveals the protein to be active. Consistent with the reported absence of a plastid in Cryptosporidium species, in silico analysis of their genomes failed to demonstrate an orthologue of prex. These studies indicate that prex is conserved across the plastid-bearing apicomplexans and may play an important role in the replication of the plastid genome.

AB - A plastid-like organelle, the apicoplast, is essential to the majority of medically and veterinary important apicomplexan protozoa including Toxoplasma gondii and Plasmodium. The apicoplast contains multiple copies of a 35 kb genome, the replication of which is dependent upon nuclear-encoded proteins that are imported into the organelle, In P. falciparum an unusual multi-functional gene, pfprex, was previously identified and inferred to encode a protein with DNA primase, DNA helicase and DNA polymerase activities. Herein, we report the presence of a prex orthologue in T. gondii. The protein is predicted to have a bi-partite apicoplast targeting sequence similar to that demonstrated on the PfPREX polypeptide, capable of delivering marker proteins to the apicoplast. Unlike the P. falciparum gene that is devoid of introns, the T. gondii prex gene carries 19 introns, which are spliced to produce a contiguous mRNA. Bacterial expression of the polymerase domain reveals the protein to be active. Consistent with the reported absence of a plastid in Cryptosporidium species, in silico analysis of their genomes failed to demonstrate an orthologue of prex. These studies indicate that prex is conserved across the plastid-bearing apicomplexans and may play an important role in the replication of the plastid genome.

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KW - DNA replication

KW - Plasmodium falciparum

KW - Toxoplasma gondii

KW - Exon

KW - Intron

KW - DNA polymerase

KW - DNA primase

KW - DNA helicase

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DO - 10.1017/S0031182009006027

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JF - Parasitology

SN - 0031-1820

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ER -