The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus.

I Kutle, sarah sengstake, Corinna Templin, Mandy Glass, T Kubsch, K Keyser, Anne Binz, Rudi Bauerfeind, Beate Sodeik, Luka Cicin-Sain, M Dezeljin, Martin Messerle

Research output: Contribution to journalArticle

Abstract

Cell rounding is a hallmark of the cytopathic effect induced by cytomegaloviruses. By screening a panel of deletion mutants of mouse cytomegalovirus (MCMV) a mutant was identified that did not elicit cell rounding and lacked the ability to form typical plaques. Altered cell morphology was assigned to the viral M25 gene. We detected an early 2.8 kb M25 mRNA directing the synthesis of a 105 kDa M25 protein, and confirmed that a late 3.1 kb mRNA encodes a 130 kDa M25 tegument protein. Virions lacking the M25 tegument protein were of smaller size because the tegument layer between capsid and viral envelope was reduced. The ΔM25 mutant did not provoke the rearrangement of the actin cytoskeleton observed after wild-type MCMV infection, and isolated expression of the M25 proteins led to cell size reduction, confirming that they contribute to the morphological changes. Yields of progeny virus and cell-to-cell spread of the ΔM25 mutant in vitro were diminished and replication in vivo was impaired. The identification of an MCMV gene involved in cell rounding provides the basis for investigating the role of this cytopathic effect in CMV pathogenesis.
Original languageEnglish
Article number15588
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 14 Nov 2017

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Muromegalovirus
Genes
Proteins
Messenger RNA
Viruses
Actins
Screening
Viral Genes
Capsid
Cytomegalovirus Infections
Cytomegalovirus
Actin Cytoskeleton
Cell Size
Virion

Keywords

  • viruses
  • cytomegalovirus infection
  • VIRUS TRANSMISSION

Cite this

Kutle, I., sengstake, S., Templin, C., Glass, M., Kubsch, T., Keyser, K., ... Messerle, M. (2017). The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus. Scientific Reports, 7(1), [15588]. https://doi.org/10.1038/s41598-017-15783-x
Kutle, I ; sengstake, sarah ; Templin, Corinna ; Glass, Mandy ; Kubsch, T ; Keyser, K ; Binz, Anne ; Bauerfeind, Rudi ; Sodeik, Beate ; Cicin-Sain, Luka ; Dezeljin, M ; Messerle, Martin. / The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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Kutle, I, sengstake, S, Templin, C, Glass, M, Kubsch, T, Keyser, K, Binz, A, Bauerfeind, R, Sodeik, B, Cicin-Sain, L, Dezeljin, M & Messerle, M 2017, 'The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus.' Scientific Reports, vol. 7, no. 1, 15588. https://doi.org/10.1038/s41598-017-15783-x

The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus. / Kutle, I; sengstake, sarah; Templin, Corinna; Glass, Mandy; Kubsch, T; Keyser, K ; Binz, Anne; Bauerfeind, Rudi; Sodeik, Beate ; Cicin-Sain, Luka; Dezeljin, M; Messerle, Martin.

In: Scientific Reports, Vol. 7, No. 1, 15588, 14.11.2017.

Research output: Contribution to journalArticle

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T1 - The M25 gene products are critical for the cytopathic effect of mouse cytomegalovirus.

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AU - Templin, Corinna

AU - Glass, Mandy

AU - Kubsch, T

AU - Keyser, K

AU - Binz, Anne

AU - Bauerfeind, Rudi

AU - Sodeik, Beate

AU - Cicin-Sain, Luka

AU - Dezeljin, M

AU - Messerle, Martin

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N2 - Cell rounding is a hallmark of the cytopathic effect induced by cytomegaloviruses. By screening a panel of deletion mutants of mouse cytomegalovirus (MCMV) a mutant was identified that did not elicit cell rounding and lacked the ability to form typical plaques. Altered cell morphology was assigned to the viral M25 gene. We detected an early 2.8 kb M25 mRNA directing the synthesis of a 105 kDa M25 protein, and confirmed that a late 3.1 kb mRNA encodes a 130 kDa M25 tegument protein. Virions lacking the M25 tegument protein were of smaller size because the tegument layer between capsid and viral envelope was reduced. The ΔM25 mutant did not provoke the rearrangement of the actin cytoskeleton observed after wild-type MCMV infection, and isolated expression of the M25 proteins led to cell size reduction, confirming that they contribute to the morphological changes. Yields of progeny virus and cell-to-cell spread of the ΔM25 mutant in vitro were diminished and replication in vivo was impaired. The identification of an MCMV gene involved in cell rounding provides the basis for investigating the role of this cytopathic effect in CMV pathogenesis.

AB - Cell rounding is a hallmark of the cytopathic effect induced by cytomegaloviruses. By screening a panel of deletion mutants of mouse cytomegalovirus (MCMV) a mutant was identified that did not elicit cell rounding and lacked the ability to form typical plaques. Altered cell morphology was assigned to the viral M25 gene. We detected an early 2.8 kb M25 mRNA directing the synthesis of a 105 kDa M25 protein, and confirmed that a late 3.1 kb mRNA encodes a 130 kDa M25 tegument protein. Virions lacking the M25 tegument protein were of smaller size because the tegument layer between capsid and viral envelope was reduced. The ΔM25 mutant did not provoke the rearrangement of the actin cytoskeleton observed after wild-type MCMV infection, and isolated expression of the M25 proteins led to cell size reduction, confirming that they contribute to the morphological changes. Yields of progeny virus and cell-to-cell spread of the ΔM25 mutant in vitro were diminished and replication in vivo was impaired. The identification of an MCMV gene involved in cell rounding provides the basis for investigating the role of this cytopathic effect in CMV pathogenesis.

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JO - Scientific Reports

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