The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase

Gary William Boyd; Marion Drew; Shannon Ward; Marianne Baird; Christopher Connaboy; Scott Murray Graham

doi:10.1139/apnm-2018-0449

The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase

Gary William Boyd, Marion Drew, Shannon Ward, Marianne Baird, Christopher Connaboy, Scott Murray Graham

School of Health and Life Sciences

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

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Abstract

Branched-chain amino acids (BCAA) are used as nutritional support for patients with a range of conditions including liver cirrhosis and in-born errors of amino acid metabolism, and are commonly used “sports”/exercise supplements. The effects of the BCAA on the in-vitro activity of calf intestinal alkaline phosphatase (EC. 3.1.3.1) were studied. All three BCAA were found to be uncompetitive inhibitors of the enzyme with L-leucine being the most potent (Ki’ = 24.9mM) and L-valine, the least potent (Ki’ = 37mM). Mixed BCAA are able to act in combination to inhibit the enzyme. Given the important role of intestinal alkaline phosphatase in gut homeostasis, these findings have potential implications for those taking high levels of BCAA as supplements.

Original language	English
Pages (from-to)	632-636
Number of pages	5
Journal	Applied Physiology, Nutrition, and Metabolism
Volume	44
Issue number	6
DOIs	https://doi.org/10.1139/apnm-2018-0449
Publication status	Published - Jun 2019

Keywords

intenstinal alkaline phosphatase
branched-chain amino acids
supplements
L-leucine
L-isoleucine
L-valine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1139/apnm-2018-0449

2018 10 14 Boyd et al BovineAccepted author manuscript, 510 KBLicence: CC BY-NC-ND

Gary Boyd
- School of Health and Life Sciences - Lecturer
Person: Academic

Cite this

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title = "The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase",

abstract = "Branched-chain amino acids (BCAA) are used as nutritional support for patients with a range of conditions including liver cirrhosis and in-born errors of amino acid metabolism, and are commonly used “sports”/exercise supplements. The effects of the BCAA on the in-vitro activity of calf intestinal alkaline phosphatase (EC. 3.1.3.1) were studied. All three BCAA were found to be uncompetitive inhibitors of the enzyme with L-leucine being the most potent (Ki{\textquoteright} = 24.9mM) and L-valine, the least potent (Ki{\textquoteright} = 37mM). Mixed BCAA are able to act in combination to inhibit the enzyme. Given the important role of intestinal alkaline phosphatase in gut homeostasis, these findings have potential implications for those taking high levels of BCAA as supplements.",

keywords = "intenstinal alkaline phosphatase, branched-chain amino acids, supplements, L-leucine, L-isoleucine, L-valine",

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year = "2019",

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T1 - The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase

AU - Boyd, Gary William

AU - Drew, Marion

AU - Ward, Shannon

AU - Baird, Marianne

AU - Connaboy, Christopher

AU - Graham, Scott Murray

PY - 2019/6

Y1 - 2019/6

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AB - Branched-chain amino acids (BCAA) are used as nutritional support for patients with a range of conditions including liver cirrhosis and in-born errors of amino acid metabolism, and are commonly used “sports”/exercise supplements. The effects of the BCAA on the in-vitro activity of calf intestinal alkaline phosphatase (EC. 3.1.3.1) were studied. All three BCAA were found to be uncompetitive inhibitors of the enzyme with L-leucine being the most potent (Ki’ = 24.9mM) and L-valine, the least potent (Ki’ = 37mM). Mixed BCAA are able to act in combination to inhibit the enzyme. Given the important role of intestinal alkaline phosphatase in gut homeostasis, these findings have potential implications for those taking high levels of BCAA as supplements.

KW - intenstinal alkaline phosphatase

KW - branched-chain amino acids

KW - supplements

KW - L-leucine

KW - L-isoleucine

KW - L-valine

U2 - 10.1139/apnm-2018-0449

DO - 10.1139/apnm-2018-0449

M3 - Article

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JO - Applied Physiology, Nutrition, and Metabolism

JF - Applied Physiology, Nutrition, and Metabolism

IS - 6

ER -

The effect of the branched-chain amino acids on the in-vitro activity of bovine intestinal alkaline phosphatase

Abstract

Keywords

UN SDGs

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Gary Boyd

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