TY - JOUR
T1 - The anti-infective potential of the endophytic fungi associated with Allium cepa supported by metabolomics analysis and docking studies
AU - Hassan, Hind Ashraf
AU - Abdelhafez, Omnia Hesham
AU - Shady, Nourhan Hisham
AU - Yahia, Ramadan
AU - Mohamed, Nada M.
AU - Rateb, Mostafa E.
AU - Akil, Lina
AU - Khadra, Ibrahim
AU - Glaeser, Stefanie P.
AU - Kämpfer, Peter
AU - Abdelmohsen, Usama Ramadan
AU - El-Katatny, Mo'men Hamed
N1 - Publisher Copyright:
© 2023 Informa UK Limited, trading as Taylor & Francis Group.
PY - 2023/1/19
Y1 - 2023/1/19
N2 - Endophytic fungi are known to be a rich source of anti-infective drugs. In our study, Allium cepa was investigated for fungal diversity using different media to give 11 isolates which were identified morphologically. Out of the isolated fungal strains, Penicillium sp. (LCEF10) revealed potential anti-infective activity against the tested microbes ( Fusarium solaniATTC 25922, Pseudomonas aeruginosa(ATTC 29231), Staphylococcus aureusATTC 27853, Candida albicansATTC 10231), besides, their MICs were measured by well diffusion method, therefore, it was subjected to molecular identification in addition to phylogenetic analysis. Moreover, the ITS sequence of strain LCEF10 showed a consistent assignment with the highest sequence similarity (99.81%) to Penicillium oxalicum NRRL 787. The crude ethyl acetate extract of Penicillium sp. LCEF10 was investigated for metabolomic analysis using LC-HR-ESI-MS. The metabolic profiling revealed the presence of polyketides, macrolides, phenolics and terpenoids. Furthermore, in silico molecular docking study was carried out to predict which compounds most likely responsible for the anti-infective activity.
AB - Endophytic fungi are known to be a rich source of anti-infective drugs. In our study, Allium cepa was investigated for fungal diversity using different media to give 11 isolates which were identified morphologically. Out of the isolated fungal strains, Penicillium sp. (LCEF10) revealed potential anti-infective activity against the tested microbes ( Fusarium solaniATTC 25922, Pseudomonas aeruginosa(ATTC 29231), Staphylococcus aureusATTC 27853, Candida albicansATTC 10231), besides, their MICs were measured by well diffusion method, therefore, it was subjected to molecular identification in addition to phylogenetic analysis. Moreover, the ITS sequence of strain LCEF10 showed a consistent assignment with the highest sequence similarity (99.81%) to Penicillium oxalicum NRRL 787. The crude ethyl acetate extract of Penicillium sp. LCEF10 was investigated for metabolomic analysis using LC-HR-ESI-MS. The metabolic profiling revealed the presence of polyketides, macrolides, phenolics and terpenoids. Furthermore, in silico molecular docking study was carried out to predict which compounds most likely responsible for the anti-infective activity.
KW - Allium
KW - Antimicrobial
KW - endophytic fungi
KW - metabolomic profiling
KW - molecular docking
UR - http://www.scopus.com/inward/record.url?scp=85146689283&partnerID=8YFLogxK
U2 - 10.1080/14786419.2023.2167204
DO - 10.1080/14786419.2023.2167204
M3 - Article
C2 - 36657413
SN - 1478-6419
VL - 37
SP - 4063
EP - 4068
JO - Natural Product Research
JF - Natural Product Research
IS - 23
ER -