Tel/PDGFR beta inhibits self-renewal and directs myelomonocytic differentiation of ES cells

E. Dobbin; P. M. Corrigan; C. P. Walsh; M. J. Welham; R. W. Freeburn; H. Wheadon

doi:10.1016/j.leukres.2008.02.007

Tel/PDGFR beta inhibits self-renewal and directs myelomonocytic differentiation of ES cells

E. Dobbin, P. M. Corrigan, C. P. Walsh, M. J. Welham, R. W. Freeburn, H. Wheadon

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The leukemic oncogene Tel/PDGFR beta, was inducibly expressed in embryonic stem (ES) cells and the phenotypic and molecular changes occurring during hematopoietic differentiation investigated. Expression of Tel/PDGFR beta resulted in an inability of ES cells to self-renew and caused a significant increase in myelopoiesis with a corresponding decrease in erythropoiesis. Analysis of gene expression patterns indicated a dramatic alteration in the levels of genes associated with self-renewal and differentiation, especially myelomonocytic genes in Tel/PDGFR beta-expressing cells. This study indicates Tel/PDGFR beta drives myelopoiesis by altering expression of genes involved in hematopoiesis and demonstrates the potential of this stem cell system to study oncogene-induced pathogenesis.

Original language	English
Pages (from-to)	1554-1564
Number of pages	11
Journal	Leukemia Research
Volume	32
Issue number	10
DOIs	https://doi.org/10.1016/j.leukres.2008.02.007
Publication status	Published - Oct 2008
Externally published	Yes

Keywords

leukemia
tyrosine kinase
Tel/PDGFR beta
embryonic stem cell
gene expression

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10.1016/j.leukres.2008.02.007

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title = "Tel/PDGFR beta inhibits self-renewal and directs myelomonocytic differentiation of ES cells",

abstract = "The leukemic oncogene Tel/PDGFR beta, was inducibly expressed in embryonic stem (ES) cells and the phenotypic and molecular changes occurring during hematopoietic differentiation investigated. Expression of Tel/PDGFR beta resulted in an inability of ES cells to self-renew and caused a significant increase in myelopoiesis with a corresponding decrease in erythropoiesis. Analysis of gene expression patterns indicated a dramatic alteration in the levels of genes associated with self-renewal and differentiation, especially myelomonocytic genes in Tel/PDGFR beta-expressing cells. This study indicates Tel/PDGFR beta drives myelopoiesis by altering expression of genes involved in hematopoiesis and demonstrates the potential of this stem cell system to study oncogene-induced pathogenesis.",

keywords = "leukemia, tyrosine kinase, Tel/PDGFR beta, embryonic stem cell, gene expression",

author = "E. Dobbin and Corrigan, {P. M.} and Walsh, {C. P.} and Welham, {M. J.} and Freeburn, {R. W.} and H. Wheadon",

year = "2008",

month = oct,

doi = "10.1016/j.leukres.2008.02.007",

language = "English",

volume = "32",

pages = "1554--1564",

journal = "Leukemia Research",

issn = "0145-2126",

publisher = "Elsevier B.V.",

number = "10",

}

TY - JOUR

T1 - Tel/PDGFR beta inhibits self-renewal and directs myelomonocytic differentiation of ES cells

AU - Dobbin, E.

AU - Corrigan, P. M.

AU - Walsh, C. P.

AU - Welham, M. J.

AU - Freeburn, R. W.

AU - Wheadon, H.

PY - 2008/10

Y1 - 2008/10

N2 - The leukemic oncogene Tel/PDGFR beta, was inducibly expressed in embryonic stem (ES) cells and the phenotypic and molecular changes occurring during hematopoietic differentiation investigated. Expression of Tel/PDGFR beta resulted in an inability of ES cells to self-renew and caused a significant increase in myelopoiesis with a corresponding decrease in erythropoiesis. Analysis of gene expression patterns indicated a dramatic alteration in the levels of genes associated with self-renewal and differentiation, especially myelomonocytic genes in Tel/PDGFR beta-expressing cells. This study indicates Tel/PDGFR beta drives myelopoiesis by altering expression of genes involved in hematopoiesis and demonstrates the potential of this stem cell system to study oncogene-induced pathogenesis.

AB - The leukemic oncogene Tel/PDGFR beta, was inducibly expressed in embryonic stem (ES) cells and the phenotypic and molecular changes occurring during hematopoietic differentiation investigated. Expression of Tel/PDGFR beta resulted in an inability of ES cells to self-renew and caused a significant increase in myelopoiesis with a corresponding decrease in erythropoiesis. Analysis of gene expression patterns indicated a dramatic alteration in the levels of genes associated with self-renewal and differentiation, especially myelomonocytic genes in Tel/PDGFR beta-expressing cells. This study indicates Tel/PDGFR beta drives myelopoiesis by altering expression of genes involved in hematopoiesis and demonstrates the potential of this stem cell system to study oncogene-induced pathogenesis.

KW - leukemia

KW - tyrosine kinase

KW - Tel/PDGFR beta

KW - embryonic stem cell

KW - gene expression

U2 - 10.1016/j.leukres.2008.02.007

DO - 10.1016/j.leukres.2008.02.007

M3 - Article

SN - 0145-2126

VL - 32

SP - 1554

EP - 1564

JO - Leukemia Research

JF - Leukemia Research

IS - 10

ER -

Tel/PDGFR beta inhibits self-renewal and directs myelomonocytic differentiation of ES cells

Abstract

Keywords

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