Second external quality assessment of the molecular diagnostic of West Nile virus: Are there improvements towards the detection of WNV?

Sonja Linke, William G. MacKay, Calum Scott, Paul Wallace, Matthias Niedrig

Research output: Contribution to journalArticle

Abstract

Background: WNV epidemics occur worldwide, new WNV isolates were isolated in southern-east Europe belonging to WNV lineage 2. A first international proficiency study on WNV indicted that some laboratories were not able to detect WNV lineage 2 virus genome by their PCR diagnostic assays. Therefore an actual External Quality Assessment with both virus lineages was performed to monitor the improvements in molecular diagnostics.

Objectives: To asses the proficiency of laboratories to detect West Nile virus with molecular diagnostic tests.

Study design: A test panel of different WNV isolates and virus dilutions was given to 26 laboratories to test the samples with their routine diagnostic methods.

Results: Twenty-one participating laboratories provided 28 data set results. WNV lineage 1 was detected with high overall efficiency of 92% (67.9-100%) but two different WNV lineage 2 strains were detected at lower rates (mean = 73%, 67.9-75%) by the different PCR assays. 93% of the laboratories were able to detect a WNV lineage 1 with a concentration of 1.2 x 10(4) copies/ml but the detection rate was decreased to 68% for 1.2 x 10(3) copies/ml. One laboratory generated false-positive result from the non-virus control samples and 29% of the datasets showed false-positive results for non-WNV flavivirus samples.

Conclusions: The WNV EQA showed an improved proficiency of laboratories as compared to the first EQA. However, the data suggest that problems in the detection of both lineages were still present since the first proficiency test was performed in 2006. Further proceedings versus the detection of both lineages are needed particularly for in-house assays. (C) 2011 Elsevier B. V. All rights reserved.
Original languageEnglish
Pages (from-to)257-260
JournalJournal of Clinical Virology
Volume52
Issue number3
DOIs
Publication statusPublished - Nov 2011

Keywords

  • West Nile virus
  • External quality assessment
  • Molecular diagnostics

Cite this

@article{9462e1196dc7429e9747693c901f6fcc,
title = "Second external quality assessment of the molecular diagnostic of West Nile virus: Are there improvements towards the detection of WNV?",
abstract = "Background: WNV epidemics occur worldwide, new WNV isolates were isolated in southern-east Europe belonging to WNV lineage 2. A first international proficiency study on WNV indicted that some laboratories were not able to detect WNV lineage 2 virus genome by their PCR diagnostic assays. Therefore an actual External Quality Assessment with both virus lineages was performed to monitor the improvements in molecular diagnostics. Objectives: To asses the proficiency of laboratories to detect West Nile virus with molecular diagnostic tests. Study design: A test panel of different WNV isolates and virus dilutions was given to 26 laboratories to test the samples with their routine diagnostic methods. Results: Twenty-one participating laboratories provided 28 data set results. WNV lineage 1 was detected with high overall efficiency of 92{\%} (67.9-100{\%}) but two different WNV lineage 2 strains were detected at lower rates (mean = 73{\%}, 67.9-75{\%}) by the different PCR assays. 93{\%} of the laboratories were able to detect a WNV lineage 1 with a concentration of 1.2 x 10(4) copies/ml but the detection rate was decreased to 68{\%} for 1.2 x 10(3) copies/ml. One laboratory generated false-positive result from the non-virus control samples and 29{\%} of the datasets showed false-positive results for non-WNV flavivirus samples. Conclusions: The WNV EQA showed an improved proficiency of laboratories as compared to the first EQA. However, the data suggest that problems in the detection of both lineages were still present since the first proficiency test was performed in 2006. Further proceedings versus the detection of both lineages are needed particularly for in-house assays. (C) 2011 Elsevier B. V. All rights reserved.",
keywords = "West Nile virus, External quality assessment, Molecular diagnostics",
author = "Sonja Linke and MacKay, {William G.} and Calum Scott and Paul Wallace and Matthias Niedrig",
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language = "English",
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Second external quality assessment of the molecular diagnostic of West Nile virus: Are there improvements towards the detection of WNV? / Linke, Sonja; MacKay, William G.; Scott, Calum; Wallace, Paul; Niedrig, Matthias.

In: Journal of Clinical Virology, Vol. 52, No. 3, 11.2011, p. 257-260.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Second external quality assessment of the molecular diagnostic of West Nile virus: Are there improvements towards the detection of WNV?

AU - Linke, Sonja

AU - MacKay, William G.

AU - Scott, Calum

AU - Wallace, Paul

AU - Niedrig, Matthias

PY - 2011/11

Y1 - 2011/11

N2 - Background: WNV epidemics occur worldwide, new WNV isolates were isolated in southern-east Europe belonging to WNV lineage 2. A first international proficiency study on WNV indicted that some laboratories were not able to detect WNV lineage 2 virus genome by their PCR diagnostic assays. Therefore an actual External Quality Assessment with both virus lineages was performed to monitor the improvements in molecular diagnostics. Objectives: To asses the proficiency of laboratories to detect West Nile virus with molecular diagnostic tests. Study design: A test panel of different WNV isolates and virus dilutions was given to 26 laboratories to test the samples with their routine diagnostic methods. Results: Twenty-one participating laboratories provided 28 data set results. WNV lineage 1 was detected with high overall efficiency of 92% (67.9-100%) but two different WNV lineage 2 strains were detected at lower rates (mean = 73%, 67.9-75%) by the different PCR assays. 93% of the laboratories were able to detect a WNV lineage 1 with a concentration of 1.2 x 10(4) copies/ml but the detection rate was decreased to 68% for 1.2 x 10(3) copies/ml. One laboratory generated false-positive result from the non-virus control samples and 29% of the datasets showed false-positive results for non-WNV flavivirus samples. Conclusions: The WNV EQA showed an improved proficiency of laboratories as compared to the first EQA. However, the data suggest that problems in the detection of both lineages were still present since the first proficiency test was performed in 2006. Further proceedings versus the detection of both lineages are needed particularly for in-house assays. (C) 2011 Elsevier B. V. All rights reserved.

AB - Background: WNV epidemics occur worldwide, new WNV isolates were isolated in southern-east Europe belonging to WNV lineage 2. A first international proficiency study on WNV indicted that some laboratories were not able to detect WNV lineage 2 virus genome by their PCR diagnostic assays. Therefore an actual External Quality Assessment with both virus lineages was performed to monitor the improvements in molecular diagnostics. Objectives: To asses the proficiency of laboratories to detect West Nile virus with molecular diagnostic tests. Study design: A test panel of different WNV isolates and virus dilutions was given to 26 laboratories to test the samples with their routine diagnostic methods. Results: Twenty-one participating laboratories provided 28 data set results. WNV lineage 1 was detected with high overall efficiency of 92% (67.9-100%) but two different WNV lineage 2 strains were detected at lower rates (mean = 73%, 67.9-75%) by the different PCR assays. 93% of the laboratories were able to detect a WNV lineage 1 with a concentration of 1.2 x 10(4) copies/ml but the detection rate was decreased to 68% for 1.2 x 10(3) copies/ml. One laboratory generated false-positive result from the non-virus control samples and 29% of the datasets showed false-positive results for non-WNV flavivirus samples. Conclusions: The WNV EQA showed an improved proficiency of laboratories as compared to the first EQA. However, the data suggest that problems in the detection of both lineages were still present since the first proficiency test was performed in 2006. Further proceedings versus the detection of both lineages are needed particularly for in-house assays. (C) 2011 Elsevier B. V. All rights reserved.

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KW - External quality assessment

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M3 - Article

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SP - 257

EP - 260

JO - Journal of Clinical Virology

JF - Journal of Clinical Virology

SN - 1386-6532

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ER -