Screening of fruits of seven plants indicated for medicinal use in Iraq

Omar Aldulaimi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)
18 Downloads (Pure)

Abstract

Introduction
Coumarins exert many biological effects in humans, animals, and plants, which make the evaluation of their biological activities and study of their role in ethnomedicine highly valued.
Objectives
Here, we selected seven plants which have ethnopharmacological use as antimicrobial in Iraq and the aims were to quantify the two structural isomers bergapten and methoxsalen in their seeds, to evaluate the antibacterial activities against several clinical isolates, and to isolate bergapten and methoxsalen from Ammi majus.
Materials and Methods
Seven plants were extracted by petroleum ether (PE) and ethanol (EtOH). Bergapten and methoxsalen were separated and purified by preparative thin-layer chromatography. Quantification of the furanocoumarins has been conducted by high-performance liquid chromatography, and all the plant extracts and pure compounds were checked for antibacterial activities utilizing alamar blue microplate assay.
Results

Cuminum cyminum was deprived of bergapten and methoxsalen and methoxsalen was not detected from Apium graveolens. Bergapten was abundant in PE more than in EtOH; on the other hand, EtOH was rich in methoxsalen. The separation of the two structural isomers was performed using normal phase chromatography and ultraviolet light as an indicator. All extracts showed weak to moderate antibacterial activities against Gram-positive isolates which were more sensitive than the negative ones. C. cyminum extract was least active, uncover the antibacterial role of bergapten and methoxsalen.
Conclusion
These findings support the medicinal use of seeds of seven plants from Apiaceae family and quantify the two pharmacologically important furanocoumarins (bergapten and methoxsalen).
Original languageEnglish
Pages (from-to)189-195
Number of pages7
JournalPharmacognosy Magazine
Volume13
Issue number50
DOIs
Publication statusPublished - 11 Jul 2017
Externally publishedYes

Keywords

  • alamar blue
  • apiaceae
  • bergapten
  • high-performance liquid chromatography
  • methoxsalen
  • thin-layer chromatography

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