Screening for fusarium antagonistic bacteria from contrasting niches designated the endophyte bacillus halotolerans as plant warden against fusarium

Houda Ben Slama, Hafsa Cherif-Silini, Ali Chenari Bouket, Mallique Qader, Allaoua Silini, Bilal Yahiaoui, Faizah N. Alenezi, Lenka Luptakova, Mohamed Ali Triki, Armelle Vallat, Tomasz Oszako, Mostafa E. Rateb, Lassaad Belbahri

Research output: Contribution to journalArticle

Abstract

Date palm (Phoenix dactylifera L.) plantations in North Africa are nowadays threatened with the spread of the Bayoud disease caused by Fusarium oxysporum f. sp. albedinis that already wiped out dates production in infected areas mainly in Morocco. Biological control holds great promise for sustainable and environmental-friendly management of the disease. In this study, using plant growth promoting rhizobacteria (PGPR) or endophytes additional benefits to agricultural ecosystems are addressed. First, PGPR or endophytes can offer an interesting bio-fertilization mean that can add another layer to the sustainability of the approach. Additionally, screening of contrasting niches can yield bacterial actors that could represent wardens against whole genera or groups of plant pathogenic agents thriving in the semi-arid to arid ecosystems. Using this strategy, we recovered four bacterial isolates designated BFOA1, BFOA2, BFOA3 and BFOA4 that proved very active against Fusarium oxysporum f. sp. albedinis. BFOA1–BFOA4 proved also active against 16 Fusarium isolates belonging to three species Fusarium oxysporum (with strains phytopathogenic of Olea europeae and tomato), Fusarium solani (with different strains attacking Olea europaea and potato), Fusarium acuminatum (pathogenic on Olea europeae) and Fusarium chlamydosporum phytopathogenic of Olea europeae). BFOA1-BFOA4 bacterial isolates exhibited strong activities against another four major phytopathogens Botrytis cinerea, Alternaria alternata, Phytophthora infestans and Rhizoctonia bataticola. Isolates BFOA1–BFOA4 had the ability to grow at temperatures up to 35 °C, pH range of 5–10, and tolerate high concentrations of NaCl and up to 30% PEG. The isolates also showed relevant direct and indirect PGP features including growth on nitrogen-free medium, phosphate solubilization and auxin biosynthesis, as well as resistance to metal and xenobiotic stress. Phylogenomic analysis of BFOA1–BFOA4 isolates indicated that they all belong to Bacillus halotolerans that could therefore considered as warden against Fusarium infection in plants. Comparative genomics allowed to functionally describe the open pan genome of B. halotolerans and LC-HRMS and GCMS analyses allowed the description of diverse secondary metabolites including: pulegone, 2-undecanone and germacrene D with important antimicrobial and insecticidal properties. In conclusion, B. halotolerans could be used as efficient bio-fertilizer and bio-control agent in semi-arid and arid ecosystems.
LanguageEnglish
Article number3236
Number of pages24
JournalFrontiers in Microbiology
Volume9
DOIs
StatePublished - 11 Jan 2019

Fingerprint

Endophytes
Fusarium
Bacillus
Bacteria
Olea
Ecosystem
Growth
Fusariosis
Phytophthora infestans
Rhizoctonia
Botrytis
Northern Africa
Morocco
Alternaria
Indoleacetic Acids
Fertilizers
Xenobiotics
Lycopersicon esculentum
Disease Management
Solanum tuberosum

Keywords

  • Phoenix dactylifera
  • Fusarium oxysporum f. sp. albedinis
  • Bayoud disease
  • endophyte
  • secretome
  • volatilome
  • comparative genomics
  • pan genome

Cite this

Slama, H. B., Cherif-Silini, H., Chenari Bouket, A., Qader, M., Silini, A., Yahiaoui, B., ... Belbahri, L. (2019). Screening for fusarium antagonistic bacteria from contrasting niches designated the endophyte bacillus halotolerans as plant warden against fusarium. Frontiers in Microbiology, 9, [3236]. DOI: 10.3389/fmicb.2018.03236
Slama, Houda Ben ; Cherif-Silini, Hafsa ; Chenari Bouket, Ali ; Qader, Mallique ; Silini, Allaoua ; Yahiaoui, Bilal ; Alenezi, Faizah N. ; Luptakova, Lenka ; Triki, Mohamed Ali ; Vallat, Armelle ; Oszako, Tomasz ; Rateb, Mostafa E. ; Belbahri, Lassaad. / Screening for fusarium antagonistic bacteria from contrasting niches designated the endophyte bacillus halotolerans as plant warden against fusarium. In: Frontiers in Microbiology. 2019 ; Vol. 9.
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abstract = "Date palm (Phoenix dactylifera L.) plantations in North Africa are nowadays threatened with the spread of the Bayoud disease caused by Fusarium oxysporum f. sp. albedinis that already wiped out dates production in infected areas mainly in Morocco. Biological control holds great promise for sustainable and environmental-friendly management of the disease. In this study, using plant growth promoting rhizobacteria (PGPR) or endophytes additional benefits to agricultural ecosystems are addressed. First, PGPR or endophytes can offer an interesting bio-fertilization mean that can add another layer to the sustainability of the approach. Additionally, screening of contrasting niches can yield bacterial actors that could represent wardens against whole genera or groups of plant pathogenic agents thriving in the semi-arid to arid ecosystems. Using this strategy, we recovered four bacterial isolates designated BFOA1, BFOA2, BFOA3 and BFOA4 that proved very active against Fusarium oxysporum f. sp. albedinis. BFOA1–BFOA4 proved also active against 16 Fusarium isolates belonging to three species Fusarium oxysporum (with strains phytopathogenic of Olea europeae and tomato), Fusarium solani (with different strains attacking Olea europaea and potato), Fusarium acuminatum (pathogenic on Olea europeae) and Fusarium chlamydosporum phytopathogenic of Olea europeae). BFOA1-BFOA4 bacterial isolates exhibited strong activities against another four major phytopathogens Botrytis cinerea, Alternaria alternata, Phytophthora infestans and Rhizoctonia bataticola. Isolates BFOA1–BFOA4 had the ability to grow at temperatures up to 35 °C, pH range of 5–10, and tolerate high concentrations of NaCl and up to 30\{%} PEG. The isolates also showed relevant direct and indirect PGP features including growth on nitrogen-free medium, phosphate solubilization and auxin biosynthesis, as well as resistance to metal and xenobiotic stress. Phylogenomic analysis of BFOA1–BFOA4 isolates indicated that they all belong to Bacillus halotolerans that could therefore considered as warden against Fusarium infection in plants. Comparative genomics allowed to functionally describe the open pan genome of B. halotolerans and LC-HRMS and GCMS analyses allowed the description of diverse secondary metabolites including: pulegone, 2-undecanone and germacrene D with important antimicrobial and insecticidal properties. In conclusion, B. halotolerans could be used as efficient bio-fertilizer and bio-control agent in semi-arid and arid ecosystems.",
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author = "Slama, {Houda Ben} and Hafsa Cherif-Silini and {Chenari Bouket}, Ali and Mallique Qader and Allaoua Silini and Bilal Yahiaoui and Alenezi, {Faizah N.} and Lenka Luptakova and Triki, {Mohamed Ali} and Armelle Vallat and Tomasz Oszako and Rateb, {Mostafa E.} and Lassaad Belbahri",
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doi = "10.3389/fmicb.2018.03236",
language = "English",
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journal = "Frontiers in Microbiology",
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Slama, HB, Cherif-Silini, H, Chenari Bouket, A, Qader, M, Silini, A, Yahiaoui, B, Alenezi, FN, Luptakova, L, Triki, MA, Vallat, A, Oszako, T, Rateb, ME & Belbahri, L 2019, 'Screening for fusarium antagonistic bacteria from contrasting niches designated the endophyte bacillus halotolerans as plant warden against fusarium' Frontiers in Microbiology, vol 9, 3236. DOI: 10.3389/fmicb.2018.03236

Screening for fusarium antagonistic bacteria from contrasting niches designated the endophyte bacillus halotolerans as plant warden against fusarium. / Slama, Houda Ben; Cherif-Silini, Hafsa; Chenari Bouket, Ali; Qader, Mallique; Silini, Allaoua; Yahiaoui, Bilal; Alenezi, Faizah N.; Luptakova, Lenka; Triki, Mohamed Ali; Vallat, Armelle; Oszako, Tomasz; Rateb, Mostafa E.; Belbahri, Lassaad.

In: Frontiers in Microbiology, Vol. 9, 3236, 11.01.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Screening for fusarium antagonistic bacteria from contrasting niches designated the endophyte bacillus halotolerans as plant warden against fusarium

AU - Slama,Houda Ben

AU - Cherif-Silini,Hafsa

AU - Chenari Bouket,Ali

AU - Qader,Mallique

AU - Silini,Allaoua

AU - Yahiaoui,Bilal

AU - Alenezi,Faizah N.

AU - Luptakova,Lenka

AU - Triki,Mohamed Ali

AU - Vallat,Armelle

AU - Oszako,Tomasz

AU - Rateb,Mostafa E.

AU - Belbahri,Lassaad

PY - 2019/1/11

Y1 - 2019/1/11

N2 - Date palm (Phoenix dactylifera L.) plantations in North Africa are nowadays threatened with the spread of the Bayoud disease caused by Fusarium oxysporum f. sp. albedinis that already wiped out dates production in infected areas mainly in Morocco. Biological control holds great promise for sustainable and environmental-friendly management of the disease. In this study, using plant growth promoting rhizobacteria (PGPR) or endophytes additional benefits to agricultural ecosystems are addressed. First, PGPR or endophytes can offer an interesting bio-fertilization mean that can add another layer to the sustainability of the approach. Additionally, screening of contrasting niches can yield bacterial actors that could represent wardens against whole genera or groups of plant pathogenic agents thriving in the semi-arid to arid ecosystems. Using this strategy, we recovered four bacterial isolates designated BFOA1, BFOA2, BFOA3 and BFOA4 that proved very active against Fusarium oxysporum f. sp. albedinis. BFOA1–BFOA4 proved also active against 16 Fusarium isolates belonging to three species Fusarium oxysporum (with strains phytopathogenic of Olea europeae and tomato), Fusarium solani (with different strains attacking Olea europaea and potato), Fusarium acuminatum (pathogenic on Olea europeae) and Fusarium chlamydosporum phytopathogenic of Olea europeae). BFOA1-BFOA4 bacterial isolates exhibited strong activities against another four major phytopathogens Botrytis cinerea, Alternaria alternata, Phytophthora infestans and Rhizoctonia bataticola. Isolates BFOA1–BFOA4 had the ability to grow at temperatures up to 35 °C, pH range of 5–10, and tolerate high concentrations of NaCl and up to 30% PEG. The isolates also showed relevant direct and indirect PGP features including growth on nitrogen-free medium, phosphate solubilization and auxin biosynthesis, as well as resistance to metal and xenobiotic stress. Phylogenomic analysis of BFOA1–BFOA4 isolates indicated that they all belong to Bacillus halotolerans that could therefore considered as warden against Fusarium infection in plants. Comparative genomics allowed to functionally describe the open pan genome of B. halotolerans and LC-HRMS and GCMS analyses allowed the description of diverse secondary metabolites including: pulegone, 2-undecanone and germacrene D with important antimicrobial and insecticidal properties. In conclusion, B. halotolerans could be used as efficient bio-fertilizer and bio-control agent in semi-arid and arid ecosystems.

AB - Date palm (Phoenix dactylifera L.) plantations in North Africa are nowadays threatened with the spread of the Bayoud disease caused by Fusarium oxysporum f. sp. albedinis that already wiped out dates production in infected areas mainly in Morocco. Biological control holds great promise for sustainable and environmental-friendly management of the disease. In this study, using plant growth promoting rhizobacteria (PGPR) or endophytes additional benefits to agricultural ecosystems are addressed. First, PGPR or endophytes can offer an interesting bio-fertilization mean that can add another layer to the sustainability of the approach. Additionally, screening of contrasting niches can yield bacterial actors that could represent wardens against whole genera or groups of plant pathogenic agents thriving in the semi-arid to arid ecosystems. Using this strategy, we recovered four bacterial isolates designated BFOA1, BFOA2, BFOA3 and BFOA4 that proved very active against Fusarium oxysporum f. sp. albedinis. BFOA1–BFOA4 proved also active against 16 Fusarium isolates belonging to three species Fusarium oxysporum (with strains phytopathogenic of Olea europeae and tomato), Fusarium solani (with different strains attacking Olea europaea and potato), Fusarium acuminatum (pathogenic on Olea europeae) and Fusarium chlamydosporum phytopathogenic of Olea europeae). BFOA1-BFOA4 bacterial isolates exhibited strong activities against another four major phytopathogens Botrytis cinerea, Alternaria alternata, Phytophthora infestans and Rhizoctonia bataticola. Isolates BFOA1–BFOA4 had the ability to grow at temperatures up to 35 °C, pH range of 5–10, and tolerate high concentrations of NaCl and up to 30% PEG. The isolates also showed relevant direct and indirect PGP features including growth on nitrogen-free medium, phosphate solubilization and auxin biosynthesis, as well as resistance to metal and xenobiotic stress. Phylogenomic analysis of BFOA1–BFOA4 isolates indicated that they all belong to Bacillus halotolerans that could therefore considered as warden against Fusarium infection in plants. Comparative genomics allowed to functionally describe the open pan genome of B. halotolerans and LC-HRMS and GCMS analyses allowed the description of diverse secondary metabolites including: pulegone, 2-undecanone and germacrene D with important antimicrobial and insecticidal properties. In conclusion, B. halotolerans could be used as efficient bio-fertilizer and bio-control agent in semi-arid and arid ecosystems.

KW - Phoenix dactylifera

KW - Fusarium oxysporum f. sp. albedinis

KW - Bayoud disease

KW - endophyte

KW - secretome

KW - volatilome

KW - comparative genomics

KW - pan genome

U2 - 10.3389/fmicb.2018.03236

DO - 10.3389/fmicb.2018.03236

M3 - Article

VL - 9

JO - Frontiers in Microbiology

T2 - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 3236

ER -