Abstract

Bacterial biofilms pose a significant burden in both healthcare and industrial environments. With the limited effectiveness of current biofilm control strategies, novel or adjunctive methods in biofilm control are being actively pursued. Reported here, is the first evidence of the application of nanovibrational stimulation (“nanokicking”) to reduce the biofilm formation of Pseudomonas aeruginosa. Nanoscale vertical displacements (approximately 60 nm) were imposed on P. aeruginosa cultures, with a significant reduction in biomass formation observed at frequencies between 200 to 4000 Hz at 24 h. The optimal reduction of biofilm formation was observed at 1 kHz, with changes in the physical morphology of the biofilms. Scanning electron microscope imaging of control and biofilms formed under nanovibrational stimulation gave indication of a reduction in extracellular matrix (ECM). Quantification of the carbohydrate and protein components of the ECM was performed and showed a significant reduction at 24 h at 1 kHz frequency. To model the forces being exerted by nanovibrational stimulation, laser interferometry was performed to measure the amplitudes produced across the Petri dish surfaces. Estimated peak forces on each cell, associated with the nanovibrational stimulation technique, were calculated to be in the order of 10 pN during initial biofilm formation. This represents a potential method of controlling microbial biofilm formation in a number of important settings in industry and medical related processes.
Original languageEnglish
JournalJournal of Bioscience and Bioengineering
Early online date15 Oct 2019
DOIs
Publication statusE-pub ahead of print - 15 Oct 2019

Fingerprint

Biofilms
Vibration
Pseudomonas aeruginosa
Interferometry
Laser interferometry
Extracellular Matrix Proteins
Carbohydrates
Biomass
Extracellular Matrix
Industry
Lasers
Electron microscopes
Electrons
Proteins
Scanning
Delivery of Health Care
Imaging techniques

Keywords

  • Nanovibration
  • Biofilm formation
  • Pseudomonas aeruginosa
  • Extracellular matrix
  • Nanokicking
  • Mechanotransduction

Cite this

Robertson, Shaun N. ; Childs, Peter G. ; Akinbobola, Ayorinde ; Henriquez, Fiona L. ; Ramage, Gordon ; Reid, Stuart ; MacKay, William G. ; Williams, Craig. / Reduction of Pseudomonas aeruginosa biofilm formation through the application of nanoscale vibration. In: Journal of Bioscience and Bioengineering. 2019.
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abstract = "Bacterial biofilms pose a significant burden in both healthcare and industrial environments. With the limited effectiveness of current biofilm control strategies, novel or adjunctive methods in biofilm control are being actively pursued. Reported here, is the first evidence of the application of nanovibrational stimulation (“nanokicking”) to reduce the biofilm formation of Pseudomonas aeruginosa. Nanoscale vertical displacements (approximately 60 nm) were imposed on P. aeruginosa cultures, with a significant reduction in biomass formation observed at frequencies between 200 to 4000 Hz at 24 h. The optimal reduction of biofilm formation was observed at 1 kHz, with changes in the physical morphology of the biofilms. Scanning electron microscope imaging of control and biofilms formed under nanovibrational stimulation gave indication of a reduction in extracellular matrix (ECM). Quantification of the carbohydrate and protein components of the ECM was performed and showed a significant reduction at 24 h at 1 kHz frequency. To model the forces being exerted by nanovibrational stimulation, laser interferometry was performed to measure the amplitudes produced across the Petri dish surfaces. Estimated peak forces on each cell, associated with the nanovibrational stimulation technique, were calculated to be in the order of 10 pN during initial biofilm formation. This represents a potential method of controlling microbial biofilm formation in a number of important settings in industry and medical related processes.",
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Reduction of Pseudomonas aeruginosa biofilm formation through the application of nanoscale vibration. / Robertson, Shaun N.; Childs, Peter G.; Akinbobola, Ayorinde; Henriquez, Fiona L.; Ramage, Gordon; Reid, Stuart; MacKay, William G.; Williams, Craig.

In: Journal of Bioscience and Bioengineering, 15.10.2019.

Research output: Contribution to journalArticle

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AU - Robertson, Shaun N.

AU - Childs, Peter G.

AU - Akinbobola, Ayorinde

AU - Henriquez, Fiona L.

AU - Ramage, Gordon

AU - Reid, Stuart

AU - MacKay, William G.

AU - Williams, Craig

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AB - Bacterial biofilms pose a significant burden in both healthcare and industrial environments. With the limited effectiveness of current biofilm control strategies, novel or adjunctive methods in biofilm control are being actively pursued. Reported here, is the first evidence of the application of nanovibrational stimulation (“nanokicking”) to reduce the biofilm formation of Pseudomonas aeruginosa. Nanoscale vertical displacements (approximately 60 nm) were imposed on P. aeruginosa cultures, with a significant reduction in biomass formation observed at frequencies between 200 to 4000 Hz at 24 h. The optimal reduction of biofilm formation was observed at 1 kHz, with changes in the physical morphology of the biofilms. Scanning electron microscope imaging of control and biofilms formed under nanovibrational stimulation gave indication of a reduction in extracellular matrix (ECM). Quantification of the carbohydrate and protein components of the ECM was performed and showed a significant reduction at 24 h at 1 kHz frequency. To model the forces being exerted by nanovibrational stimulation, laser interferometry was performed to measure the amplitudes produced across the Petri dish surfaces. Estimated peak forces on each cell, associated with the nanovibrational stimulation technique, were calculated to be in the order of 10 pN during initial biofilm formation. This represents a potential method of controlling microbial biofilm formation in a number of important settings in industry and medical related processes.

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