Pseudomonas aeruginosa and their small diffusible extracellular molecules inhibit Aspergillus fumigatus biofilm formation

Eilidh Mowat, Ranjith Rajendran, Craig Williams, Elaine McCulloch, Brian L. Jones, Sue Lang, Gordon Ramage

Research output: Contribution to journalArticle

Abstract

Aspergillus fumigatus is often isolated from the lungs of cystic fibrosis (CF) patients, but unlike in severely immunocompromised individuals, the mortality rates are low. This suggests that competition from bacteria within the CF lung may be inhibitory. The purpose of this study was to investigate how Pseudomonas aeruginosa influences A. fumigatus conidial germination and biofilm formation. Aspergillus fumigatus biofilm formation was inhibited by direct contact with P. aeruginosa, but had no effect on preformed biofilm. A secreted heat-stable soluble factor was also shown to exhibit biofilm inhibition. Coculture of P. aeruginosa quorum-sensing mutants (PAO1:ΔLasI, PAO1:ΔLasR) did not significantly inhibit A. fumigatus biofilms (52.6-58.8%) to the same extent as that of the PA01 wild type (22.9-30.1%), both by direct and by indirect interaction (P<0.001). Planktonic and sessile inhibition assays with a series of short carbon chain molecules (decanol, decanoic acid and dodecanol) demonstrated that these molecules could both inhibit and disrupt biofilms in a concentration-dependent manner. Overall, this suggests that small diffusible and heat-stable molecules may be responsible for the competitive inhibition of filamentous fungal growth in polymicrobial environments such as the CF lung.

Original languageEnglish
Pages (from-to)96-102
Number of pages7
JournalFEMS Microbiology Letters
Volume313
Issue number2
DOIs
Publication statusPublished - Dec 2010
Externally publishedYes

Fingerprint

Aspergillus fumigatus
Biofilms
Pseudomonas aeruginosa
Cystic Fibrosis
Lung
Dodecanol
Hot Temperature
Quorum Sensing
Germination
Coculture Techniques
Carbon
Bacteria
Mortality
Growth

Keywords

  • Antibiosis
  • Antifungal Agents
  • Aspergillus fumigatus
  • Biofilms
  • Gene Deletion
  • Genes, Bacterial
  • Humans
  • Pseudomonas aeruginosa
  • Quorum Sensing
  • Journal Article

Cite this

Mowat, Eilidh ; Rajendran, Ranjith ; Williams, Craig ; McCulloch, Elaine ; Jones, Brian L. ; Lang, Sue ; Ramage, Gordon. / Pseudomonas aeruginosa and their small diffusible extracellular molecules inhibit Aspergillus fumigatus biofilm formation. In: FEMS Microbiology Letters. 2010 ; Vol. 313, No. 2. pp. 96-102.
@article{e469b079d3c44a46ab980f1f4ad2c06e,
title = "Pseudomonas aeruginosa and their small diffusible extracellular molecules inhibit Aspergillus fumigatus biofilm formation",
abstract = "Aspergillus fumigatus is often isolated from the lungs of cystic fibrosis (CF) patients, but unlike in severely immunocompromised individuals, the mortality rates are low. This suggests that competition from bacteria within the CF lung may be inhibitory. The purpose of this study was to investigate how Pseudomonas aeruginosa influences A. fumigatus conidial germination and biofilm formation. Aspergillus fumigatus biofilm formation was inhibited by direct contact with P. aeruginosa, but had no effect on preformed biofilm. A secreted heat-stable soluble factor was also shown to exhibit biofilm inhibition. Coculture of P. aeruginosa quorum-sensing mutants (PAO1:ΔLasI, PAO1:ΔLasR) did not significantly inhibit A. fumigatus biofilms (52.6-58.8{\%}) to the same extent as that of the PA01 wild type (22.9-30.1{\%}), both by direct and by indirect interaction (P<0.001). Planktonic and sessile inhibition assays with a series of short carbon chain molecules (decanol, decanoic acid and dodecanol) demonstrated that these molecules could both inhibit and disrupt biofilms in a concentration-dependent manner. Overall, this suggests that small diffusible and heat-stable molecules may be responsible for the competitive inhibition of filamentous fungal growth in polymicrobial environments such as the CF lung.",
keywords = "Antibiosis, Antifungal Agents, Aspergillus fumigatus, Biofilms, Gene Deletion, Genes, Bacterial, Humans, Pseudomonas aeruginosa, Quorum Sensing, Journal Article",
author = "Eilidh Mowat and Ranjith Rajendran and Craig Williams and Elaine McCulloch and Jones, {Brian L.} and Sue Lang and Gordon Ramage",
note = "{\circledC} 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.",
year = "2010",
month = "12",
doi = "10.1111/j.1574-6968.2010.02130.x",
language = "English",
volume = "313",
pages = "96--102",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "2",

}

Pseudomonas aeruginosa and their small diffusible extracellular molecules inhibit Aspergillus fumigatus biofilm formation. / Mowat, Eilidh; Rajendran, Ranjith; Williams, Craig; McCulloch, Elaine; Jones, Brian L. ; Lang, Sue; Ramage, Gordon.

In: FEMS Microbiology Letters, Vol. 313, No. 2, 12.2010, p. 96-102.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Pseudomonas aeruginosa and their small diffusible extracellular molecules inhibit Aspergillus fumigatus biofilm formation

AU - Mowat, Eilidh

AU - Rajendran, Ranjith

AU - Williams, Craig

AU - McCulloch, Elaine

AU - Jones, Brian L.

AU - Lang, Sue

AU - Ramage, Gordon

N1 - © 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

PY - 2010/12

Y1 - 2010/12

N2 - Aspergillus fumigatus is often isolated from the lungs of cystic fibrosis (CF) patients, but unlike in severely immunocompromised individuals, the mortality rates are low. This suggests that competition from bacteria within the CF lung may be inhibitory. The purpose of this study was to investigate how Pseudomonas aeruginosa influences A. fumigatus conidial germination and biofilm formation. Aspergillus fumigatus biofilm formation was inhibited by direct contact with P. aeruginosa, but had no effect on preformed biofilm. A secreted heat-stable soluble factor was also shown to exhibit biofilm inhibition. Coculture of P. aeruginosa quorum-sensing mutants (PAO1:ΔLasI, PAO1:ΔLasR) did not significantly inhibit A. fumigatus biofilms (52.6-58.8%) to the same extent as that of the PA01 wild type (22.9-30.1%), both by direct and by indirect interaction (P<0.001). Planktonic and sessile inhibition assays with a series of short carbon chain molecules (decanol, decanoic acid and dodecanol) demonstrated that these molecules could both inhibit and disrupt biofilms in a concentration-dependent manner. Overall, this suggests that small diffusible and heat-stable molecules may be responsible for the competitive inhibition of filamentous fungal growth in polymicrobial environments such as the CF lung.

AB - Aspergillus fumigatus is often isolated from the lungs of cystic fibrosis (CF) patients, but unlike in severely immunocompromised individuals, the mortality rates are low. This suggests that competition from bacteria within the CF lung may be inhibitory. The purpose of this study was to investigate how Pseudomonas aeruginosa influences A. fumigatus conidial germination and biofilm formation. Aspergillus fumigatus biofilm formation was inhibited by direct contact with P. aeruginosa, but had no effect on preformed biofilm. A secreted heat-stable soluble factor was also shown to exhibit biofilm inhibition. Coculture of P. aeruginosa quorum-sensing mutants (PAO1:ΔLasI, PAO1:ΔLasR) did not significantly inhibit A. fumigatus biofilms (52.6-58.8%) to the same extent as that of the PA01 wild type (22.9-30.1%), both by direct and by indirect interaction (P<0.001). Planktonic and sessile inhibition assays with a series of short carbon chain molecules (decanol, decanoic acid and dodecanol) demonstrated that these molecules could both inhibit and disrupt biofilms in a concentration-dependent manner. Overall, this suggests that small diffusible and heat-stable molecules may be responsible for the competitive inhibition of filamentous fungal growth in polymicrobial environments such as the CF lung.

KW - Antibiosis

KW - Antifungal Agents

KW - Aspergillus fumigatus

KW - Biofilms

KW - Gene Deletion

KW - Genes, Bacterial

KW - Humans

KW - Pseudomonas aeruginosa

KW - Quorum Sensing

KW - Journal Article

U2 - 10.1111/j.1574-6968.2010.02130.x

DO - 10.1111/j.1574-6968.2010.02130.x

M3 - Article

VL - 313

SP - 96

EP - 102

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -