Proteinase activated receptor 2 (PAR2) modulation of airway smooth muscle function

Kimberly Black, Andrew MacKenzie, Lynette Dunning, Anne Crilly, Lorcan McGarvey, Keith Thornbury, Carl Goodyear, John Lockhart, Gary Litherland

Research output: Contribution to journalMeeting Abstract

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Abstract

Chronic obstructive pulmonary disease (COPD) is a progressive lung condition characterised by airflow obstruction and irreversible lung damage. Hyperactivity and growth of airway smooth muscle (ASM) limit airflow and are key features of COPD. Proteinase activated receptor-2 (PAR2) is a key modulator of inflammatory responses in respiratory disease, such as asthma and promotes ASM relaxation. However, the precise role of the receptor in ASM in conditions such as COPD is not well understood (1). The aim of this study was to establish an ex vivo murine airway myograph assay for investigation of functional PAR2 responses to challenges relevant in COPD pathology. To achieve this, murine trachea and bronchi tissue was either mounted on a wire myograph for dynamic tension recording or processed for immunohistochemical localisation of PAR2 expression. PAR2 was present on both murine airway and lung tissue. Stimulation of PAR2 with trypsin (10 U ml-1) or activating peptide was observed to induce relaxation responses in ASM tension. Taken together this data verifies that PAR2 is present and functional in murine airways, and ex vivo modulation alters ASM tone. Ongoing experiments will investigate the effect of disease-relevant insults on this modulation in wild type and PAR2-deficient airways.
Original languageEnglish
Pages (from-to)S249-S249
Number of pages1
JournalIrish Journal of Medical Science
Volume187
Issue numberSupplement 8
DOIs
Publication statusPublished - 8 Oct 2018

Keywords

  • PAR2
  • COPD
  • BREATH
  • interreg va
  • SEUPB
  • airway smooth muscle

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  • Activities

    • 1 Participation in workshop, seminar, course
    • 1 Types of Public engagement and outreach - Public lecture/debate/seminar
    • 1 Oral presentation

    Cross Party Group on Lung Health, Scottish Parliament (attending member)

    John Lockhart (Invited speaker)

    12 Jun 2018

    Activity: Participating in or organising an eventParticipation in workshop, seminar, course

    BREATH Ayrshire Stakeholder Event

    John Lockhart (Speaker), , Gary Litherland (Speaker), , Anur Guhan (Speaker), , Lorcan McGarvey (Speaker), , Keith Thornbury (Speaker), & Iain McLellan (Speaker)

    22 Aug 2018

    Activity: Talk or presentationOral presentation

    BREATH Dumfries and Galloway Launch Event

    Anne Crilly (Participant), , Gary Litherland (Speaker), & John Lockhart (Speaker)

    Aug 2017

    Activity: OtherTypes of Public engagement and outreach - Public lecture/debate/seminar

    Prizes

    Asthma/COPD Project of the Year, 19th Northern Ireland Healthcare Awards 2018

    Lorraine Martin (Recipient), Lorcan McGarvey (Recipient), Fionnuala Lundy (Recipient), Litherland, Gary (Recipient), Lockhart, John (Recipient), Crilly, Anne (Recipient), Carl Goodyear (Recipient), Keith Thornbury (Recipient), Mark Hollywood (Recipient), Gerard Sergeant (Recipient) & Noel McHale (Recipient), 22 Feb 2018

    Prize: National/international honour

    Northern Ireland Healthcare Awards

    Lockhart, John (Recipient), 22 Feb 2018

    Prize: Prize (including medals and awards)

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