Activities per year
To investigate this, primary human epithelial cells and epithelial cell lines were stimulated with PAR2 activators (SLIGKV, trypsin) and autophagy quantified using a fluorescent marker of autophagosomes (CYTO-ID detection kit). Western blotting to analyse expression of autophagyrelated proteins was used to confirm findings. PAR2 expression was assessed by immunofluorescence.
We confirmed that PAR2 was on human airway epithelial cells. Autophagic vesicles were successfully detected and modulated by appropriate autophagy control stimuli. Stimulation of PAR2, assessed alone and in combination with inflammatory stimuli, resulted in modulation of the level of autophagic activity within airway epithelial cultures.
Our study provides the first data that suggests a role for PAR2 in the regulation of autophagy in lung airway epithelia. This further suggests a possible novel targetable mechanism underlying conditions such as COPD that feature premature lung ageing
|Number of pages||2|
|Journal||Irish Journal of Medical Science|
|Issue number||Supplement 8|
|Publication status||Published - 8 Oct 2018|
|Event||Irish Thoracic Society Annual Scientific Meeting - Hastings Europa Hotel, Belfast, United Kingdom|
Duration: 23 Nov 2018 → 24 Nov 2018
- interreg va
- airway epithelial cells
FingerprintDive into the research topics of 'Proteinase activated receptor-2 and autophagy regulation in airway epithelia cells'. Together they form a unique fingerprint.
24 Sept 2018 → 28 Sept 2018
John Lockhart, Gary Litherland, Anne Crilly, Carl Goodyear, Andrew MacKenzie, Iain McLellan, Andisheh Bakhshi, Lynette Dunning, Robin Freeburn, William MacKay, Craig Williams, Kirsty McCallum, Mariarca Bailo, Kimberly Black, Bryn Short, Mark Thomas & Fawziye Tarhini
1 Media contribution
1 item of Media coverage
Martin, Lorraine (Recipient), McGarvey, Lorcan (Recipient), Lundy, Fionnuala (Recipient), Litherland, Gary (Recipient), Lockhart, John (Recipient), Crilly, Anne (Recipient), Goodyear, Carl (Recipient), Thornbury, Keith (Recipient), Hollywood, Mark (Recipient), Sergeant, Gerard (Recipient) & McHale, Noel (Recipient), 22 Feb 2018
Prize: National/international honour
Lockhart, John (Recipient), 22 Feb 2018
Prize: Prize (including medals and awards)