Protein kinase D3 modulates MMP1 and MMP13 expression in human chondrocytes

Jonathan Baker, Adrian Falconer, David J. Wilkinson, G. Nicholas Europe-Finner, Gary Litherland, Andrew D. Rowan

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Many catabolic stimuli, including interleukin-1 (IL-1) in combination with oncostatin M (OSM), promote cartilage breakdown via the induction of collagen-degrading collagenases such as matrix metalloproteinase 1 (MMP1) and MMP13 in human articular chondrocytes. Indeed, joint diseases with an inflammatory component are characterised by excessive extracellular matrix (ECM) catabolism. Importantly, protein kinase C (PKC) signalling has a primary role in cytokine-induced MMP1/13 expression, and is known to regulate cellular functions associated with pathologies involving ECM remodelling. At present, substrates downstream of PKC remain undefined. Herein, we show that both IL-1- and OSM-induced phosphorylation of protein kinase D (PKD) in human chondrocytes is strongly associated with signalling via the atypical PKCι isoform. Consequently, inhibiting PKD activation with a pan-PKD inhibitor significantly reduced the expression of MMP1/13. Specific gene silencing of the PKD isoforms revealed that only PKD3 (PRKD3) depletion mirrored the observed MMP repression, indicative of the pharmacological inhibitor specifically affecting only this isoform. PRKD3 silencing was also shown to reduce serine phosphorylation of signal transducer and activator of transcription 3 (STAT3) as well as phosphorylation of all three mitogen-activated protein kinase groups. This altered signalling following PRKD3 silencing led to a significant reduction in the expression of the activator protein-1 (AP-1) genes FOS and JUN, critical for the induction of many MMPs including MMP1/13. Furthermore, the AP-1 factor activating transcription factor 3 (ATF3) was also reduced concomitant with the observed reduction in MMP13 expression. Taken together, we highlight an important role for PKD3 in the pro-inflammatory signalling that promotes cartilage destruction.
Original languageEnglish
Article numbere0195864
Number of pages19
JournalPLoS ONE
Volume13
Issue number4
DOIs
Publication statusPublished - 13 Apr 2018

Keywords

  • Cartilage, Articular
  • Osteoarthritis
  • Collagenases
  • Extracellular Matrix
  • Gene Expression Regulation

Fingerprint

Dive into the research topics of 'Protein kinase D3 modulates MMP1 and MMP13 expression in human chondrocytes'. Together they form a unique fingerprint.

Cite this