Precise control of the assembly of dye-coded oligonucleotide silver nanoparticle conjugates with single base mismatch discrimination using surface enhanced resonance Raman scattering

David G. Thompson, Karen Faulds, W. Ewen Smith, Duncan Graham

Research output: Contribution to journalArticle

Abstract

The control of the assembly of nanostructures of dye-coded oligonucleotide silver nanoparticle (DOSN) conjugates (silver nanoparticles cofunctionalized with thiol modified DNA and a Raman reporter molecule) in a sandwich assay format with single base discrimination in an unmodified target oligonucleotide is reported. Rational placement of a single base mismatch in the 18 base duplex formed between target and DOSN conjugates completely controls the hybridization event, preventing or allowing nanostructure formation. The assembly process was monitored using UV-vis spectrometry and surface-enhanced resonance Raman scattering (SERRS). These two supporting analysis techniques show that there is an initial formation of small, highly SERRS active assemblies followed by the formation of larger superaggregates. The rapid increase in SERRS intensity obtained from DOSN conjugate hybridization has been utilized for the near-immediate discrimination of a single base mismatch using SERRS. This report highlights the exquisite control and detection possibilities offered by coupling specific molecular recognition events, such as DNA hybridization, with the optical and SERRS responses possible by nanoparticle assembly.
Original languageEnglish
Pages (from-to)7384-7389
Number of pages6
JournalJournal of Physical Chemistry C
Volume114
Issue number16
DOIs
Publication statusPublished - 29 Apr 2010
Externally publishedYes

Cite this

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title = "Precise control of the assembly of dye-coded oligonucleotide silver nanoparticle conjugates with single base mismatch discrimination using surface enhanced resonance Raman scattering",
abstract = "The control of the assembly of nanostructures of dye-coded oligonucleotide silver nanoparticle (DOSN) conjugates (silver nanoparticles cofunctionalized with thiol modified DNA and a Raman reporter molecule) in a sandwich assay format with single base discrimination in an unmodified target oligonucleotide is reported. Rational placement of a single base mismatch in the 18 base duplex formed between target and DOSN conjugates completely controls the hybridization event, preventing or allowing nanostructure formation. The assembly process was monitored using UV-vis spectrometry and surface-enhanced resonance Raman scattering (SERRS). These two supporting analysis techniques show that there is an initial formation of small, highly SERRS active assemblies followed by the formation of larger superaggregates. The rapid increase in SERRS intensity obtained from DOSN conjugate hybridization has been utilized for the near-immediate discrimination of a single base mismatch using SERRS. This report highlights the exquisite control and detection possibilities offered by coupling specific molecular recognition events, such as DNA hybridization, with the optical and SERRS responses possible by nanoparticle assembly.",
author = "Thompson, {David G.} and Karen Faulds and Smith, {W. Ewen} and Duncan Graham",
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T1 - Precise control of the assembly of dye-coded oligonucleotide silver nanoparticle conjugates with single base mismatch discrimination using surface enhanced resonance Raman scattering

AU - Thompson, David G.

AU - Faulds, Karen

AU - Smith, W. Ewen

AU - Graham, Duncan

PY - 2010/4/29

Y1 - 2010/4/29

N2 - The control of the assembly of nanostructures of dye-coded oligonucleotide silver nanoparticle (DOSN) conjugates (silver nanoparticles cofunctionalized with thiol modified DNA and a Raman reporter molecule) in a sandwich assay format with single base discrimination in an unmodified target oligonucleotide is reported. Rational placement of a single base mismatch in the 18 base duplex formed between target and DOSN conjugates completely controls the hybridization event, preventing or allowing nanostructure formation. The assembly process was monitored using UV-vis spectrometry and surface-enhanced resonance Raman scattering (SERRS). These two supporting analysis techniques show that there is an initial formation of small, highly SERRS active assemblies followed by the formation of larger superaggregates. The rapid increase in SERRS intensity obtained from DOSN conjugate hybridization has been utilized for the near-immediate discrimination of a single base mismatch using SERRS. This report highlights the exquisite control and detection possibilities offered by coupling specific molecular recognition events, such as DNA hybridization, with the optical and SERRS responses possible by nanoparticle assembly.

AB - The control of the assembly of nanostructures of dye-coded oligonucleotide silver nanoparticle (DOSN) conjugates (silver nanoparticles cofunctionalized with thiol modified DNA and a Raman reporter molecule) in a sandwich assay format with single base discrimination in an unmodified target oligonucleotide is reported. Rational placement of a single base mismatch in the 18 base duplex formed between target and DOSN conjugates completely controls the hybridization event, preventing or allowing nanostructure formation. The assembly process was monitored using UV-vis spectrometry and surface-enhanced resonance Raman scattering (SERRS). These two supporting analysis techniques show that there is an initial formation of small, highly SERRS active assemblies followed by the formation of larger superaggregates. The rapid increase in SERRS intensity obtained from DOSN conjugate hybridization has been utilized for the near-immediate discrimination of a single base mismatch using SERRS. This report highlights the exquisite control and detection possibilities offered by coupling specific molecular recognition events, such as DNA hybridization, with the optical and SERRS responses possible by nanoparticle assembly.

U2 - 10.1021/jp909639b

DO - 10.1021/jp909639b

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SP - 7384

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JO - Journal of Physical Chemistry C

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SN - 1932-7447

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