Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation

Farah Jaber-Hijazi, Priscilla J.K.P. Lo, Yuliana Mihaylova, Jeremy M. Foster, Jack S. Benner, Belen Tejada Romero, Chen Chen, Sunir Malla, Jordi Solana, Alexey Ruzov, A. Aziz Aboobaker*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)
21 Downloads (Pure)

Abstract

Planarian adult stem cells (pASCs) or neoblasts represent an ideal system to study the evolution of stem cells and pluripotency as they underpin an unrivaled capacity for regeneration. We wish to understand the control of differentiation and pluripotency in pASCs and to understand how conserved, convergent or divergent these mechanisms are across the Bilateria. Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis. The genome does not have detectable levels of 5-methylcytosine (5mC) and we find no role for a potential DNA methylase. We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation.
Original languageEnglish
Pages (from-to)141-153
Number of pages13
JournalDevelopmental Biology
Volume384
Issue number1
Early online date21 Sep 2013
DOIs
Publication statusPublished - 1 Dec 2013
Externally publishedYes

Keywords

  • Methyl binding domain
  • Pluripotency
  • Differentiation
  • Regeneration

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