Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation

Farah  Jaber-Hijazi; Priscilla J.K.P. Lo; Yuliana Mihaylova; Jeremy M. Foster; Jack S. Benner; Belen Tejada Romero; Chen Chen; Sunir Malla; Jordi Solana; Alexey Ruzov; A. Aziz Aboobaker

doi:10.1016/j.ydbio.2013.09.020

Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation

Farah Jaber-Hijazi, Priscilla J.K.P. Lo, Yuliana Mihaylova, Jeremy M. Foster, Jack S. Benner, Belen Tejada Romero, Chen Chen, Sunir Malla, Jordi Solana, Alexey Ruzov, A. Aziz Aboobaker^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Planarian adult stem cells (pASCs) or neoblasts represent an ideal system to study the evolution of stem cells and pluripotency as they underpin an unrivaled capacity for regeneration. We wish to understand the control of differentiation and pluripotency in pASCs and to understand how conserved, convergent or divergent these mechanisms are across the Bilateria. Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis. The genome does not have detectable levels of 5-methylcytosine (5^mC) and we find no role for a potential DNA methylase. We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation.

Original language	English
Pages (from-to)	141-153
Number of pages	13
Journal	Developmental Biology
Volume	384
Issue number	1
Early online date	21 Sept 2013
DOIs	https://doi.org/10.1016/j.ydbio.2013.09.020
Publication status	Published - 1 Dec 2013
Externally published	Yes

Keywords

Methyl binding domain
Pluripotency
Differentiation
Regeneration

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10.1016/j.ydbio.2013.09.020Licence: CC BY

2013 09 16 Jaber-Hijazi et al Planarian finalFinal published version, 9.42 MBLicence: CC BY

Cite this

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title = "Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation",

abstract = "Planarian adult stem cells (pASCs) or neoblasts represent an ideal system to study the evolution of stem cells and pluripotency as they underpin an unrivaled capacity for regeneration. We wish to understand the control of differentiation and pluripotency in pASCs and to understand how conserved, convergent or divergent these mechanisms are across the Bilateria. Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis. The genome does not have detectable levels of 5-methylcytosine (5mC) and we find no role for a potential DNA methylase. We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation.",

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author = "Farah Jaber-Hijazi and Lo, {Priscilla J.K.P.} and Yuliana Mihaylova and Foster, {Jeremy M.} and Benner, {Jack S.} and {Tejada Romero}, Belen and Chen Chen and Sunir Malla and Jordi Solana and Alexey Ruzov and Aboobaker, {A. Aziz}",

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doi = "10.1016/j.ydbio.2013.09.020",

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journal = "Developmental Biology",

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T1 - Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation

AU - Jaber-Hijazi, Farah

AU - Lo, Priscilla J.K.P.

AU - Mihaylova, Yuliana

AU - Foster, Jeremy M.

AU - Benner, Jack S.

AU - Tejada Romero, Belen

AU - Chen, Chen

AU - Malla, Sunir

AU - Solana, Jordi

AU - Ruzov, Alexey

AU - Aboobaker, A. Aziz

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Planarian adult stem cells (pASCs) or neoblasts represent an ideal system to study the evolution of stem cells and pluripotency as they underpin an unrivaled capacity for regeneration. We wish to understand the control of differentiation and pluripotency in pASCs and to understand how conserved, convergent or divergent these mechanisms are across the Bilateria. Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis. The genome does not have detectable levels of 5-methylcytosine (5mC) and we find no role for a potential DNA methylase. We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation.

AB - Planarian adult stem cells (pASCs) or neoblasts represent an ideal system to study the evolution of stem cells and pluripotency as they underpin an unrivaled capacity for regeneration. We wish to understand the control of differentiation and pluripotency in pASCs and to understand how conserved, convergent or divergent these mechanisms are across the Bilateria. Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis. The genome does not have detectable levels of 5-methylcytosine (5mC) and we find no role for a potential DNA methylase. We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation.

KW - Methyl binding domain

KW - Pluripotency

KW - Differentiation

KW - Regeneration

U2 - 10.1016/j.ydbio.2013.09.020

DO - 10.1016/j.ydbio.2013.09.020

M3 - Article

VL - 384

SP - 141

EP - 153

JO - Developmental Biology

JF - Developmental Biology

IS - 1

ER -

Planarian MBD2/3 is required for adult stem cell pluripotency independently of DNA methylation

Abstract

Keywords

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