Placing the HIRA histone chaperone complex in the chromatin landscape

Nikolay A Pchelintsev, Tony McBryan, Taranjit Singh Rai, John van Tuyn, Dominique Ray-Gallet, Geneviève Almouzni, Peter D Adams

Research output: Contribution to journalArticle

Abstract

The HIRA chaperone complex, comprised of HIRA, UBN1, and CABIN1, collaborates with histone-binding protein ASF1a to incorporate histone variant H3.3 into chromatin in a DNA replication-independent manner. To better understand HIRA's function and mechanism, we integrated HIRA, UBN1, ASF1a, and histone H3.3 chromatin immunoprecipitation sequencing and gene expression analyses. Most HIRA-binding sites colocalize with UBN1, ASF1a, and H3.3 at active promoters and active and weak/poised enhancers. At promoters, binding of HIRA/UBN1/ASF1a correlates with the level of gene expression. HIRA is required for deposition of histone H3.3 at its binding sites. There are marked differences in nucleosome and coregulator composition at different classes of HIRA-bound regulatory sites. Underscoring this, we report physical interactions between the HIRA complex and transcription factors, a chromatin insulator and an ATP-dependent chromatin-remodeling complex. Our results map the distribution of the HIRA chaperone across the chromatin landscape and point to different interacting partners at functionally distinct regulatory sites.

Original languageEnglish
Pages (from-to)1012-9
Number of pages8
JournalCell Reports
Volume3
Issue number4
DOIs
Publication statusPublished - 25 Apr 2013
Externally publishedYes

Fingerprint

Histone Chaperones
Histones
Chromatin
Gene expression
Binding Sites
Gene Expression
Chromatin Assembly and Disassembly
Nucleosomes
Chromatin Immunoprecipitation
DNA Replication
Carrier Proteins
Transcription Factors
Adenosine Triphosphate
DNA
Chemical analysis

Keywords

  • Binding Sites
  • Cell Cycle Proteins
  • Chromatin
  • Chromatin Immunoprecipitation
  • Cluster Analysis
  • Genetic Loci
  • HeLa Cells
  • Histone Chaperones
  • Histones
  • Humans
  • Nuclear Proteins
  • Promoter Regions, Genetic
  • RNA Interference
  • RNA, Small Interfering
  • Transcription Factors

Cite this

Pchelintsev, N. A., McBryan, T., Rai, T. S., van Tuyn, J., Ray-Gallet, D., Almouzni, G., & Adams, P. D. (2013). Placing the HIRA histone chaperone complex in the chromatin landscape. Cell Reports, 3(4), 1012-9. https://doi.org/10.1016/j.celrep.2013.03.026
Pchelintsev, Nikolay A ; McBryan, Tony ; Rai, Taranjit Singh ; van Tuyn, John ; Ray-Gallet, Dominique ; Almouzni, Geneviève ; Adams, Peter D. / Placing the HIRA histone chaperone complex in the chromatin landscape. In: Cell Reports. 2013 ; Vol. 3, No. 4. pp. 1012-9.
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Pchelintsev, NA, McBryan, T, Rai, TS, van Tuyn, J, Ray-Gallet, D, Almouzni, G & Adams, PD 2013, 'Placing the HIRA histone chaperone complex in the chromatin landscape' Cell Reports, vol. 3, no. 4, pp. 1012-9. https://doi.org/10.1016/j.celrep.2013.03.026

Placing the HIRA histone chaperone complex in the chromatin landscape. / Pchelintsev, Nikolay A; McBryan, Tony; Rai, Taranjit Singh; van Tuyn, John; Ray-Gallet, Dominique; Almouzni, Geneviève; Adams, Peter D.

In: Cell Reports, Vol. 3, No. 4, 25.04.2013, p. 1012-9.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Placing the HIRA histone chaperone complex in the chromatin landscape

AU - Pchelintsev, Nikolay A

AU - McBryan, Tony

AU - Rai, Taranjit Singh

AU - van Tuyn, John

AU - Ray-Gallet, Dominique

AU - Almouzni, Geneviève

AU - Adams, Peter D

N1 - Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2013/4/25

Y1 - 2013/4/25

N2 - The HIRA chaperone complex, comprised of HIRA, UBN1, and CABIN1, collaborates with histone-binding protein ASF1a to incorporate histone variant H3.3 into chromatin in a DNA replication-independent manner. To better understand HIRA's function and mechanism, we integrated HIRA, UBN1, ASF1a, and histone H3.3 chromatin immunoprecipitation sequencing and gene expression analyses. Most HIRA-binding sites colocalize with UBN1, ASF1a, and H3.3 at active promoters and active and weak/poised enhancers. At promoters, binding of HIRA/UBN1/ASF1a correlates with the level of gene expression. HIRA is required for deposition of histone H3.3 at its binding sites. There are marked differences in nucleosome and coregulator composition at different classes of HIRA-bound regulatory sites. Underscoring this, we report physical interactions between the HIRA complex and transcription factors, a chromatin insulator and an ATP-dependent chromatin-remodeling complex. Our results map the distribution of the HIRA chaperone across the chromatin landscape and point to different interacting partners at functionally distinct regulatory sites.

AB - The HIRA chaperone complex, comprised of HIRA, UBN1, and CABIN1, collaborates with histone-binding protein ASF1a to incorporate histone variant H3.3 into chromatin in a DNA replication-independent manner. To better understand HIRA's function and mechanism, we integrated HIRA, UBN1, ASF1a, and histone H3.3 chromatin immunoprecipitation sequencing and gene expression analyses. Most HIRA-binding sites colocalize with UBN1, ASF1a, and H3.3 at active promoters and active and weak/poised enhancers. At promoters, binding of HIRA/UBN1/ASF1a correlates with the level of gene expression. HIRA is required for deposition of histone H3.3 at its binding sites. There are marked differences in nucleosome and coregulator composition at different classes of HIRA-bound regulatory sites. Underscoring this, we report physical interactions between the HIRA complex and transcription factors, a chromatin insulator and an ATP-dependent chromatin-remodeling complex. Our results map the distribution of the HIRA chaperone across the chromatin landscape and point to different interacting partners at functionally distinct regulatory sites.

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KW - Cell Cycle Proteins

KW - Chromatin

KW - Chromatin Immunoprecipitation

KW - Cluster Analysis

KW - Genetic Loci

KW - HeLa Cells

KW - Histone Chaperones

KW - Histones

KW - Humans

KW - Nuclear Proteins

KW - Promoter Regions, Genetic

KW - RNA Interference

KW - RNA, Small Interfering

KW - Transcription Factors

U2 - 10.1016/j.celrep.2013.03.026

DO - 10.1016/j.celrep.2013.03.026

M3 - Article

VL - 3

SP - 1012

EP - 1019

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 4

ER -

Pchelintsev NA, McBryan T, Rai TS, van Tuyn J, Ray-Gallet D, Almouzni G et al. Placing the HIRA histone chaperone complex in the chromatin landscape. Cell Reports. 2013 Apr 25;3(4):1012-9. https://doi.org/10.1016/j.celrep.2013.03.026