Physiological and behavioral effects of exposure to environmentally relevant concentrations of prednisolone during zebrafish (Danio rerio) embryogenesis

Paul L. McNeil, Carolina Nebot, Katherine Sloman

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)
197 Downloads (Pure)

Abstract

The presence of synthetic glucocorticoids within the aquatic environment has been highlighted as a potential environmental concern as they may mimic the role of endogenous glucocorticoids during vertebrate ontogeny. Prednisolone is a commonly prescribed synthetic glucocorticoid which has been repeatedly detected in the environment. This study investigated the impact of environmentally relevant concentrations of prednisolone (0.1, 1, and 10 μg/L) during zebrafish embryogenesis using physiological and behavioral end points which are known to be mediated by endogenous glucocorticoids. The frequency of spontaneous muscle contractions (24 hpf) was significantly reduced by prednisolone and 0.1 μg/L increased the distance embryos swam in response to a mechanosensory stimulus (48 hpf). The percentage of embryos hatched significantly increased following prednisolone treatment (1 and 10 μg/L), while growth and mortality were unaffected. The onset of heart contraction was differentially affected by prednisolone while heart rate and oxygen consumption both increased significantly throughout embryogenesis. No substantial effect on the axial musculature was observed. Morphological changes to the lower jaw were detected at 96 hpf in response to 1 μg/L of prednisolone. Several parameters of swim behavior were also significantly affected. Environmentally relevant concentrations of prednisolone therefore alter early zebrafish ontogeny and significantly affect embryo behavior.

Original languageEnglish
Pages (from-to)5294-5304
Number of pages11
JournalEnvironmental Science and Technology
Volume50
Issue number10
Early online date28 Apr 2016
DOIs
Publication statusE-pub ahead of print - 28 Apr 2016

Fingerprint

Dive into the research topics of 'Physiological and behavioral effects of exposure to environmentally relevant concentrations of prednisolone during zebrafish (Danio rerio) embryogenesis'. Together they form a unique fingerprint.

Cite this