Periodontal treatment causes a longitudinal increase in nitrite‐producing bacteria

Background: The oral microbiome-dependent nitrate (NO₃⁻)–nitrite (NO₂⁻)–nitric oxide (NO) pathway may help regulate blood pressure. NO₂⁻-producing bacteria in subgingival plaque are reduced in relative abundance in patients with untreated periodontitis compared with periodontally healthy patients. In periodontitis patients, the NO₂⁻-producing bacteria increase several months after periodontal treatment. The early effects of periodontal treatment on NO₂⁻-producing bacteria and the NO₃⁻–NO₂⁻–NO pathway remain unknown. The aim of this study was to determine how periodontal treatment affects the oral NO₂⁻-producing microbiome and salivary NO₃⁻and NO₂⁻ levels over time.
Methods: The subgingival microbiota of 38 periodontitis patients was analysed
before (baseline [BL]) and 1, 7 and 90 days after periodontal treatment. Changes in NO₂⁻ -producing bacteria and periodontitis-associated bacteria were determined by 16s rRNA Illumina sequencing. Saliva samples were collected at all-time points to determine NO₃⁻ and NO₂⁻  levels using gas-phase chemiluminescence.
Results: A significant increase was observed in the relative abundance of NO₂⁻ -
producing species between BL and all subsequent timepoints (all p < 0.001).
Periodontitis-associated species decreased at all timepoints, relative to BL (all
p < 0.02). NO₂⁻ -producing species negatively correlated with periodontitis-associated species at all timepoints, with this relationship strongest 90 days post-treatment (ρ = −0.792, p < 0.001). Despite these findings, no significant changes were found in salivary NO₃⁻  and NO₂⁻  over time (all p > 0.05).
Conclusions: Periodontal treatment induced an immediate increase in the relative abundance of health-associated NO₂⁻ -producing bacteria. This increase persisted throughout periodontal healing. Future studies should test the effect of periodontal treatment combined with NO₃ ⁻ intake on periodontal and cardiovascular health.