Oxidative stress driven inflammatory responses in lung epithelial cells

F. Tarhini, A. Crilly, J. Brzeszczynska, L. McGarvey, K. Thornbury, C.S. Goodyear, J.C. Lockhart, G.J. Litherland

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Abstract

Cigarette smoke stimulates an inflammatory response and produces oxidants that cause oxidative stress in the lung, promoting pathophysiological changes related to chronic obstructive pulmonary disease (COPD).1 Hydrogen peroxide (H2O2) is an important oxidant detected in breath condensate of COPD patients.2 We aim to understand how chronic exposure to H2O2 alone or in combination with other inflammatory mediators influences epithelial cell responses relevant to COPD lung pathology.

BEAS-2B cells were exposed chronically to H2O2 for 2 h/day for 3 days at different concentrations, alone or in combination with TGF-β (10 ng/ml) or LPS (100 or 500 ng/ml). Cell viability was assessed by MTT assay. Cytokines were measured by ELISA. Intracellular ROS production was detected by CM-H2DCFDA assay. Data were analysed using one-way ANOVA, followed by Multiple Comparison Test.

Cells tolerated a repeated exposure of H2O2 (up to 15 μM) ± TGF-β or LPS without significant loss of viability. Intracellular ROS was significantly elevated in the presence of LPS (mean ± SEM; 217±17%; p< 0.0001) or H2O2(331±13%; p< 0.0001), with an additive effect of combined treatment (H2O2, 444±12 vs. LPS + H2O2, 604±35%; p< 0.0001). H2O2 stimulated modest release of IL-8 (38±2 pg/ml) and IL-6 (84±13 pg/ml). However, repeated 15 μM H2O2 exposure synergistically enhanced TGF-β induced IL-8 (TGF-β, 194±13 vs. TGF-β+ H2O2, 279±10 pg/ml; p< 0.0001) but not IL-6 (TGF-β, 431±22 vs. TGF-β+ H2O2, 449±2 pg/ml). H2O2 synergistically enhanced LPS secretion of both IL-8 (LPS, 2487±21 vs. LPS+ H2O2, 2898±109 pg/ml; p< 0.0001), and IL-6 (LPS, 2469±72 vs. LPS+ H2O2, 3277±62 pg/ml; p< 0.0001).

Oxidative stress appears to be generated in BEAS-2B cells by LPS or H2O2 alone, and increased in combination. Repeated exposure to H2O2 induced minimal inflammatory response, but synergistically enhanced the effect of TGF-β and LPS on cytokine production. These data suggest combined exposure models may be useful to study the effects of epithelial cell challenges relevant to COPD pathology.

References

Kirkham P, Rahman I. Oxidative stress in asthma and COPD: antioxidant as a therapeutic strategy. Pharmacol Ther 2006;111:476–94.

Montuschi, P. Exhaled breath condensate analysis in patients with COPD. Clin Chim Acta 2005;356:22–34.
Original languageEnglish
Article numberA31
JournalTHORAX
Volume76
Issue numberSuppl 1
DOIs
Publication statusPublished - 21 Jan 2021
EventBritish Thoracic Society Winter Meeting Feb 2021 - Online (COVID-19)
Duration: 17 Feb 202119 Feb 2021
https://www.brit-thoracic.org.uk/education-and-events/winter-meeting/february-2021-winter-meeting/

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