Omeprazole, Helicobacter pylori status, and alterations in the intragastric milieu facilitating bacterial N-nitrosation

Craig Mowat, Craig Williams, Derek Gillen, Margaret Hossack, Denise Gilmour, Andrew Carswell, Angela Wirz, Tom Preston, Kenneth E.L. McColl

Research output: Contribution to journalArticle

Abstract

BACKGROUND & AIMS: Omeprazole produces greater acid inhibition in Helicobacter pylori-positive than -negative subjects. We investigated whether this is accompanied by more profound changes in the intragastric milieu that facilitates bacterial synthesis of N-nitroso compounds.

METHODS: Gastric juice pH; nitrite, ascorbic acid, and total vitamin C concentrations; and colonization by other bacteria were examined before and during omeprazole treatment in subjects with and without H. pylori infection. Studies were performed in the fasting state and after consumption of 2 mmol nitrate (equivalent to a salad meal).

RESULTS: Before omeprazole, H. pylori-positive and -negative subjects were similar for all parameters. During omeprazole, H. pylori-positive subjects had a higher intragastric pH (7.8 vs. 3.0; P < 0.00001) and greater colonization with non-H. pylori species (5 x 10(7) vs. 5 x 10(5) CFU/mL; P < 0.05). These bacteria included nitrosating species. During omeprazole treatment, H. pylori-positive subjects had higher intragastric nitrite levels after the nitrate meal (median area under the concentration/time curve, 12,450 vs. 4708 micromol/L. min; P = 0.04). Omeprazole lowered intragastric vitamin C levels in H. pylori-positive but not -negative subjects (1.8 vs. 3.4 microg/mL, respectively; P = 0.02).

CONCLUSIONS: In H. pylori-positive subjects, omeprazole produces disturbances in intragastric nitrite, vitamin C, and bacterial colonization that facilitate bacterial N-nitrosation. This may place them at increased risk of mutagenesis and carcinogenesis.

Original languageEnglish
Pages (from-to)339-347
Number of pages9
JournalGastroenterology
Volume119
Issue number2
DOIs
Publication statusPublished - Aug 2000
Externally publishedYes

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Nitrosation
Omeprazole
Helicobacter pylori
Ascorbic Acid
Nitrites
Nitrates
Meals
Nitroso Compounds
Bacteria
Gastric Juice
Pylorus
Helicobacter Infections
Mutagenesis
Fasting
Carcinogenesis
Acids
Therapeutics

Keywords

  • Adult
  • Anti-Ulcer Agents
  • Ascorbic Acid
  • Female
  • Gastric Juice
  • Gastritis
  • Helicobacter Infections
  • Helicobacter pylori
  • Humans
  • Hydrogen-Ion Concentration
  • Male
  • Microbiological Techniques
  • Middle Aged
  • Nitrites
  • Nitrosation
  • Omeprazole
  • Saliva
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Mowat, Craig ; Williams, Craig ; Gillen, Derek ; Hossack, Margaret ; Gilmour, Denise ; Carswell, Andrew ; Wirz, Angela ; Preston, Tom ; McColl, Kenneth E.L. / Omeprazole, Helicobacter pylori status, and alterations in the intragastric milieu facilitating bacterial N-nitrosation. In: Gastroenterology. 2000 ; Vol. 119, No. 2. pp. 339-347.
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abstract = "BACKGROUND & AIMS: Omeprazole produces greater acid inhibition in Helicobacter pylori-positive than -negative subjects. We investigated whether this is accompanied by more profound changes in the intragastric milieu that facilitates bacterial synthesis of N-nitroso compounds.METHODS: Gastric juice pH; nitrite, ascorbic acid, and total vitamin C concentrations; and colonization by other bacteria were examined before and during omeprazole treatment in subjects with and without H. pylori infection. Studies were performed in the fasting state and after consumption of 2 mmol nitrate (equivalent to a salad meal).RESULTS: Before omeprazole, H. pylori-positive and -negative subjects were similar for all parameters. During omeprazole, H. pylori-positive subjects had a higher intragastric pH (7.8 vs. 3.0; P < 0.00001) and greater colonization with non-H. pylori species (5 x 10(7) vs. 5 x 10(5) CFU/mL; P < 0.05). These bacteria included nitrosating species. During omeprazole treatment, H. pylori-positive subjects had higher intragastric nitrite levels after the nitrate meal (median area under the concentration/time curve, 12,450 vs. 4708 micromol/L. min; P = 0.04). Omeprazole lowered intragastric vitamin C levels in H. pylori-positive but not -negative subjects (1.8 vs. 3.4 microg/mL, respectively; P = 0.02).CONCLUSIONS: In H. pylori-positive subjects, omeprazole produces disturbances in intragastric nitrite, vitamin C, and bacterial colonization that facilitate bacterial N-nitrosation. This may place them at increased risk of mutagenesis and carcinogenesis.",
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author = "Craig Mowat and Craig Williams and Derek Gillen and Margaret Hossack and Denise Gilmour and Andrew Carswell and Angela Wirz and Tom Preston and McColl, {Kenneth E.L.}",
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Mowat, C, Williams, C, Gillen, D, Hossack, M, Gilmour, D, Carswell, A, Wirz, A, Preston, T & McColl, KEL 2000, 'Omeprazole, Helicobacter pylori status, and alterations in the intragastric milieu facilitating bacterial N-nitrosation' Gastroenterology, vol. 119, no. 2, pp. 339-347. https://doi.org/10.1053/gast.2000.9367

Omeprazole, Helicobacter pylori status, and alterations in the intragastric milieu facilitating bacterial N-nitrosation. / Mowat, Craig; Williams, Craig; Gillen, Derek; Hossack, Margaret; Gilmour, Denise; Carswell, Andrew; Wirz, Angela; Preston, Tom; McColl, Kenneth E.L.

In: Gastroenterology, Vol. 119, No. 2, 08.2000, p. 339-347.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Omeprazole, Helicobacter pylori status, and alterations in the intragastric milieu facilitating bacterial N-nitrosation

AU - Mowat, Craig

AU - Williams, Craig

AU - Gillen, Derek

AU - Hossack, Margaret

AU - Gilmour, Denise

AU - Carswell, Andrew

AU - Wirz, Angela

AU - Preston, Tom

AU - McColl, Kenneth E.L.

PY - 2000/8

Y1 - 2000/8

N2 - BACKGROUND & AIMS: Omeprazole produces greater acid inhibition in Helicobacter pylori-positive than -negative subjects. We investigated whether this is accompanied by more profound changes in the intragastric milieu that facilitates bacterial synthesis of N-nitroso compounds.METHODS: Gastric juice pH; nitrite, ascorbic acid, and total vitamin C concentrations; and colonization by other bacteria were examined before and during omeprazole treatment in subjects with and without H. pylori infection. Studies were performed in the fasting state and after consumption of 2 mmol nitrate (equivalent to a salad meal).RESULTS: Before omeprazole, H. pylori-positive and -negative subjects were similar for all parameters. During omeprazole, H. pylori-positive subjects had a higher intragastric pH (7.8 vs. 3.0; P < 0.00001) and greater colonization with non-H. pylori species (5 x 10(7) vs. 5 x 10(5) CFU/mL; P < 0.05). These bacteria included nitrosating species. During omeprazole treatment, H. pylori-positive subjects had higher intragastric nitrite levels after the nitrate meal (median area under the concentration/time curve, 12,450 vs. 4708 micromol/L. min; P = 0.04). Omeprazole lowered intragastric vitamin C levels in H. pylori-positive but not -negative subjects (1.8 vs. 3.4 microg/mL, respectively; P = 0.02).CONCLUSIONS: In H. pylori-positive subjects, omeprazole produces disturbances in intragastric nitrite, vitamin C, and bacterial colonization that facilitate bacterial N-nitrosation. This may place them at increased risk of mutagenesis and carcinogenesis.

AB - BACKGROUND & AIMS: Omeprazole produces greater acid inhibition in Helicobacter pylori-positive than -negative subjects. We investigated whether this is accompanied by more profound changes in the intragastric milieu that facilitates bacterial synthesis of N-nitroso compounds.METHODS: Gastric juice pH; nitrite, ascorbic acid, and total vitamin C concentrations; and colonization by other bacteria were examined before and during omeprazole treatment in subjects with and without H. pylori infection. Studies were performed in the fasting state and after consumption of 2 mmol nitrate (equivalent to a salad meal).RESULTS: Before omeprazole, H. pylori-positive and -negative subjects were similar for all parameters. During omeprazole, H. pylori-positive subjects had a higher intragastric pH (7.8 vs. 3.0; P < 0.00001) and greater colonization with non-H. pylori species (5 x 10(7) vs. 5 x 10(5) CFU/mL; P < 0.05). These bacteria included nitrosating species. During omeprazole treatment, H. pylori-positive subjects had higher intragastric nitrite levels after the nitrate meal (median area under the concentration/time curve, 12,450 vs. 4708 micromol/L. min; P = 0.04). Omeprazole lowered intragastric vitamin C levels in H. pylori-positive but not -negative subjects (1.8 vs. 3.4 microg/mL, respectively; P = 0.02).CONCLUSIONS: In H. pylori-positive subjects, omeprazole produces disturbances in intragastric nitrite, vitamin C, and bacterial colonization that facilitate bacterial N-nitrosation. This may place them at increased risk of mutagenesis and carcinogenesis.

KW - Adult

KW - Anti-Ulcer Agents

KW - Ascorbic Acid

KW - Female

KW - Gastric Juice

KW - Gastritis

KW - Helicobacter Infections

KW - Helicobacter pylori

KW - Humans

KW - Hydrogen-Ion Concentration

KW - Male

KW - Microbiological Techniques

KW - Middle Aged

KW - Nitrites

KW - Nitrosation

KW - Omeprazole

KW - Saliva

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1053/gast.2000.9367

DO - 10.1053/gast.2000.9367

M3 - Article

VL - 119

SP - 339

EP - 347

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 2

ER -