Nitrate-rich Beetroot Juice Vs. Nitrate-rich Chard Gel: A Phamacokinetic And Pharmacodynamic Comparison

Chris Easton, Luke C McIlvenna, Chris Monaghan, Bernadette O Fernandez, Martin Feelisch, David Muggeridge

Research output: Contribution to journalMeeting Abstract

Abstract

It has been suggested that the pharmacokinetics of nitrate (NO3-) may differ depending on the delivery vehicle, although this has not yet been explored in the same group of study participants.PURPOSE: To compare the changes in blood pressure (BP), plasma [NO3-], nitrite [NO2-], and total nitroso species [RXNO] following ingestion of commercially available beetroot juice (BR) and NO3- rich chard gels (NG), matched for NO3- content.METHODS: ~500 mg nitrate was administered acutely to nine healthy male adults using two different formulations in a randomised order. In one arm they ingested two 60 g NG gels and in the other 117 ml of concentrated BR instead. Samples of venous blood were collected and BP was measured prior to ingestion of the supplements and at frequent intervals for 6 h after ingestion. Blood samples were centrifuged and, following separation of the plasma, [RXNO] was measured using gas-phase chemiluminescence and [NO3-] and [NO2-] were measured using HPLC.RESULTS: Plasma [NO3-] increased to a similar extent and peaked 60 min after ingestion, in both conditions (BR Δ 376 ± 29 μM; NG Δ 332 ± 34 μM, both P<0.001). Plasma [NO2-] increased and peaked 210 min after ingestion of BR (Δ 234 ± 90 nM, P=0.04) and 90 min after NG administration (Δ 308 ± 159 nM, P=0.02), with no difference in peak values between conditions (P=0.69). RXNO peaked earlier following BR (120 min, Δ 418 ± 145 nM, P<0.01) compared to NG (180 min, Δ 171 ± 31 nM, P=0.03). The diffence in Δ RXNO between BR and NG approached statistical significance (P=0.06). Systolic BP was reduced to a similar extent in both conditions from 90 min onwards, with the largest reduction measured after 210 min (BR Δ 11 ± 1 nmmHg, P<0.01; NG Δ 11 ± 1 mmHg, P<0.01). Diastolic BP was reduced 120 min (P=0.04) and 150 min (Δ 5 ± 2 nmmHg, P=0.02) after ingestion of NG, but did not change with BR (P>0.05).CONCLUSIONS: While there were no differences in peak [NO2-], [NO3-], or the extent to which SBP was reduced between NG and BR conditions, plasma [NO2-] peaked sooner following ingestion of NG (90 min) compared to BR (210 min), and RXNO increased to a greater extent following BR. The underlying mechanism(s) for these pharmacokinetic differences are unclear, but must relate to the composition of the supplements likely affecting the reduction of NO3- to NO2- in the oral cavity and/or the gastrointestinal tract
Original languageEnglish
Pages (from-to)977
JournalMedicine & Science in Sports & Exercise
Volume48
Issue number5S
DOIs
Publication statusPublished - May 2016

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Beta vulgaris
Nitrates
Eating
Gels
Pharmacokinetics
Blood Pressure
Nitrites
Luminescence
Mouth
Gastrointestinal Tract
Arm
Gases
High Pressure Liquid Chromatography

Cite this

Easton, Chris ; McIlvenna, Luke C ; Monaghan, Chris ; Fernandez, Bernadette O ; Feelisch, Martin ; Muggeridge, David. / Nitrate-rich Beetroot Juice Vs. Nitrate-rich Chard Gel: A Phamacokinetic And Pharmacodynamic Comparison. In: Medicine & Science in Sports & Exercise. 2016 ; Vol. 48, No. 5S. pp. 977.
@article{11aba69df2ac4ac2990a44c49d03f98d,
title = "Nitrate-rich Beetroot Juice Vs. Nitrate-rich Chard Gel: A Phamacokinetic And Pharmacodynamic Comparison",
abstract = "It has been suggested that the pharmacokinetics of nitrate (NO3-) may differ depending on the delivery vehicle, although this has not yet been explored in the same group of study participants.PURPOSE: To compare the changes in blood pressure (BP), plasma [NO3-], nitrite [NO2-], and total nitroso species [RXNO] following ingestion of commercially available beetroot juice (BR) and NO3- rich chard gels (NG), matched for NO3- content.METHODS: ~500 mg nitrate was administered acutely to nine healthy male adults using two different formulations in a randomised order. In one arm they ingested two 60 g NG gels and in the other 117 ml of concentrated BR instead. Samples of venous blood were collected and BP was measured prior to ingestion of the supplements and at frequent intervals for 6 h after ingestion. Blood samples were centrifuged and, following separation of the plasma, [RXNO] was measured using gas-phase chemiluminescence and [NO3-] and [NO2-] were measured using HPLC.RESULTS: Plasma [NO3-] increased to a similar extent and peaked 60 min after ingestion, in both conditions (BR Δ 376 ± 29 μM; NG Δ 332 ± 34 μM, both P<0.001). Plasma [NO2-] increased and peaked 210 min after ingestion of BR (Δ 234 ± 90 nM, P=0.04) and 90 min after NG administration (Δ 308 ± 159 nM, P=0.02), with no difference in peak values between conditions (P=0.69). RXNO peaked earlier following BR (120 min, Δ 418 ± 145 nM, P<0.01) compared to NG (180 min, Δ 171 ± 31 nM, P=0.03). The diffence in Δ RXNO between BR and NG approached statistical significance (P=0.06). Systolic BP was reduced to a similar extent in both conditions from 90 min onwards, with the largest reduction measured after 210 min (BR Δ 11 ± 1 nmmHg, P<0.01; NG Δ 11 ± 1 mmHg, P<0.01). Diastolic BP was reduced 120 min (P=0.04) and 150 min (Δ 5 ± 2 nmmHg, P=0.02) after ingestion of NG, but did not change with BR (P>0.05).CONCLUSIONS: While there were no differences in peak [NO2-], [NO3-], or the extent to which SBP was reduced between NG and BR conditions, plasma [NO2-] peaked sooner following ingestion of NG (90 min) compared to BR (210 min), and RXNO increased to a greater extent following BR. The underlying mechanism(s) for these pharmacokinetic differences are unclear, but must relate to the composition of the supplements likely affecting the reduction of NO3- to NO2- in the oral cavity and/or the gastrointestinal tract",
author = "Chris Easton and McIlvenna, {Luke C} and Chris Monaghan and Fernandez, {Bernadette O} and Martin Feelisch and David Muggeridge",
year = "2016",
month = "5",
doi = "10.1249/01.mss.0000487934.24595.a3",
language = "English",
volume = "48",
pages = "977",
journal = "Medicine & Science in Sports & Exercise",
issn = "0195-9131",
publisher = "American College of Sports Medicine",
number = "5S",

}

Nitrate-rich Beetroot Juice Vs. Nitrate-rich Chard Gel: A Phamacokinetic And Pharmacodynamic Comparison. / Easton, Chris; McIlvenna, Luke C; Monaghan, Chris ; Fernandez, Bernadette O; Feelisch, Martin ; Muggeridge, David.

In: Medicine & Science in Sports & Exercise, Vol. 48, No. 5S, 05.2016, p. 977.

Research output: Contribution to journalMeeting Abstract

TY - JOUR

T1 - Nitrate-rich Beetroot Juice Vs. Nitrate-rich Chard Gel: A Phamacokinetic And Pharmacodynamic Comparison

AU - Easton, Chris

AU - McIlvenna, Luke C

AU - Monaghan, Chris

AU - Fernandez, Bernadette O

AU - Feelisch, Martin

AU - Muggeridge, David

PY - 2016/5

Y1 - 2016/5

N2 - It has been suggested that the pharmacokinetics of nitrate (NO3-) may differ depending on the delivery vehicle, although this has not yet been explored in the same group of study participants.PURPOSE: To compare the changes in blood pressure (BP), plasma [NO3-], nitrite [NO2-], and total nitroso species [RXNO] following ingestion of commercially available beetroot juice (BR) and NO3- rich chard gels (NG), matched for NO3- content.METHODS: ~500 mg nitrate was administered acutely to nine healthy male adults using two different formulations in a randomised order. In one arm they ingested two 60 g NG gels and in the other 117 ml of concentrated BR instead. Samples of venous blood were collected and BP was measured prior to ingestion of the supplements and at frequent intervals for 6 h after ingestion. Blood samples were centrifuged and, following separation of the plasma, [RXNO] was measured using gas-phase chemiluminescence and [NO3-] and [NO2-] were measured using HPLC.RESULTS: Plasma [NO3-] increased to a similar extent and peaked 60 min after ingestion, in both conditions (BR Δ 376 ± 29 μM; NG Δ 332 ± 34 μM, both P<0.001). Plasma [NO2-] increased and peaked 210 min after ingestion of BR (Δ 234 ± 90 nM, P=0.04) and 90 min after NG administration (Δ 308 ± 159 nM, P=0.02), with no difference in peak values between conditions (P=0.69). RXNO peaked earlier following BR (120 min, Δ 418 ± 145 nM, P<0.01) compared to NG (180 min, Δ 171 ± 31 nM, P=0.03). The diffence in Δ RXNO between BR and NG approached statistical significance (P=0.06). Systolic BP was reduced to a similar extent in both conditions from 90 min onwards, with the largest reduction measured after 210 min (BR Δ 11 ± 1 nmmHg, P<0.01; NG Δ 11 ± 1 mmHg, P<0.01). Diastolic BP was reduced 120 min (P=0.04) and 150 min (Δ 5 ± 2 nmmHg, P=0.02) after ingestion of NG, but did not change with BR (P>0.05).CONCLUSIONS: While there were no differences in peak [NO2-], [NO3-], or the extent to which SBP was reduced between NG and BR conditions, plasma [NO2-] peaked sooner following ingestion of NG (90 min) compared to BR (210 min), and RXNO increased to a greater extent following BR. The underlying mechanism(s) for these pharmacokinetic differences are unclear, but must relate to the composition of the supplements likely affecting the reduction of NO3- to NO2- in the oral cavity and/or the gastrointestinal tract

AB - It has been suggested that the pharmacokinetics of nitrate (NO3-) may differ depending on the delivery vehicle, although this has not yet been explored in the same group of study participants.PURPOSE: To compare the changes in blood pressure (BP), plasma [NO3-], nitrite [NO2-], and total nitroso species [RXNO] following ingestion of commercially available beetroot juice (BR) and NO3- rich chard gels (NG), matched for NO3- content.METHODS: ~500 mg nitrate was administered acutely to nine healthy male adults using two different formulations in a randomised order. In one arm they ingested two 60 g NG gels and in the other 117 ml of concentrated BR instead. Samples of venous blood were collected and BP was measured prior to ingestion of the supplements and at frequent intervals for 6 h after ingestion. Blood samples were centrifuged and, following separation of the plasma, [RXNO] was measured using gas-phase chemiluminescence and [NO3-] and [NO2-] were measured using HPLC.RESULTS: Plasma [NO3-] increased to a similar extent and peaked 60 min after ingestion, in both conditions (BR Δ 376 ± 29 μM; NG Δ 332 ± 34 μM, both P<0.001). Plasma [NO2-] increased and peaked 210 min after ingestion of BR (Δ 234 ± 90 nM, P=0.04) and 90 min after NG administration (Δ 308 ± 159 nM, P=0.02), with no difference in peak values between conditions (P=0.69). RXNO peaked earlier following BR (120 min, Δ 418 ± 145 nM, P<0.01) compared to NG (180 min, Δ 171 ± 31 nM, P=0.03). The diffence in Δ RXNO between BR and NG approached statistical significance (P=0.06). Systolic BP was reduced to a similar extent in both conditions from 90 min onwards, with the largest reduction measured after 210 min (BR Δ 11 ± 1 nmmHg, P<0.01; NG Δ 11 ± 1 mmHg, P<0.01). Diastolic BP was reduced 120 min (P=0.04) and 150 min (Δ 5 ± 2 nmmHg, P=0.02) after ingestion of NG, but did not change with BR (P>0.05).CONCLUSIONS: While there were no differences in peak [NO2-], [NO3-], or the extent to which SBP was reduced between NG and BR conditions, plasma [NO2-] peaked sooner following ingestion of NG (90 min) compared to BR (210 min), and RXNO increased to a greater extent following BR. The underlying mechanism(s) for these pharmacokinetic differences are unclear, but must relate to the composition of the supplements likely affecting the reduction of NO3- to NO2- in the oral cavity and/or the gastrointestinal tract

U2 - 10.1249/01.mss.0000487934.24595.a3

DO - 10.1249/01.mss.0000487934.24595.a3

M3 - Meeting Abstract

VL - 48

SP - 977

JO - Medicine & Science in Sports & Exercise

JF - Medicine & Science in Sports & Exercise

SN - 0195-9131

IS - 5S

ER -