Nanoscale stimulation of osteoblastogenesis from mesenchymal stem cells: nanotopography and nanokicking

Gabriel D. Pemberton, Peter Childs, Stuart Reid, Habib Nikukar, P. Monica Tsimbouri, Nikolaj Gadegaard, Adam S. G. Curtis, Matthew J. Dalby

Research output: Contribution to journalArticle

Abstract

Aim: Mesenchymal stem cells (MSCs) have large regenerative potential to replace damaged cells from several tissues along the mesodermal lineage. The potency of these cells promises to change the longer term prognosis for many degenerative conditions currently suffered by our aging population. We have endeavored to demonstrate our ability to induce osteoblatogenesis in MSCs using high-frequency (1000-5000 Hz) piezo-driven nanodisplacements (16-30 nm displacements) in a vertical direction. Materials & methods: Osteoblastogenesis has been determined by the upregulation of osteoblasic genes such as osteonectin (ONN), RUNX2 and Osterix, assessed via quantitative real-time PCR; the increase of osteocalcin (OCN) and osteopontin (OPN) at the protein level and the deposition of calcium phosphate determined by histological staining. Results: Intriguingly, we have observed a relationship between nanotopography and piezo-stimulated mechanotransduction and possibly see evidence of two differing osteogenic mechanisms at work. These data provide confidence in nanomechanotransduction for stem cell differentiation without dependence on soluble factors and complex chemistries. Conclusion: In the future it is envisaged that this technology may have beneficial therapeutic applications in the healthcare industry, for conditions whose overall phenotype maybe characterized by weak or damaged bones (e.g., osteoporosis and bone fractures), and which can benefit from having an increased number of osteoblastic cells in vivo.
Original languageEnglish
Pages (from-to)547-560
JournalNANOMEDICINE
Volume10
Issue number4
DOIs
Publication statusPublished - 2015

Keywords

  • mechanotransduction
  • mesenchymal stem cells
  • nanotopography
  • nanovibration
  • osteoblastogenesis
  • piezo effect
  • regenerative medicine

Cite this

Pemberton, G. D., Childs, P., Reid, S., Nikukar, H., Tsimbouri, P. M., Gadegaard, N., ... Dalby, M. J. (2015). Nanoscale stimulation of osteoblastogenesis from mesenchymal stem cells: nanotopography and nanokicking. NANOMEDICINE, 10(4), 547-560. https://doi.org/10.2217/nnm.14.134
Pemberton, Gabriel D. ; Childs, Peter ; Reid, Stuart ; Nikukar, Habib ; Tsimbouri, P. Monica ; Gadegaard, Nikolaj ; Curtis, Adam S. G. ; Dalby, Matthew J. / Nanoscale stimulation of osteoblastogenesis from mesenchymal stem cells : nanotopography and nanokicking. In: NANOMEDICINE. 2015 ; Vol. 10, No. 4. pp. 547-560.
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Pemberton, GD, Childs, P, Reid, S, Nikukar, H, Tsimbouri, PM, Gadegaard, N, Curtis, ASG & Dalby, MJ 2015, 'Nanoscale stimulation of osteoblastogenesis from mesenchymal stem cells: nanotopography and nanokicking' NANOMEDICINE, vol. 10, no. 4, pp. 547-560. https://doi.org/10.2217/nnm.14.134

Nanoscale stimulation of osteoblastogenesis from mesenchymal stem cells : nanotopography and nanokicking. / Pemberton, Gabriel D.; Childs, Peter; Reid, Stuart; Nikukar, Habib; Tsimbouri, P. Monica; Gadegaard, Nikolaj; Curtis, Adam S. G.; Dalby, Matthew J.

In: NANOMEDICINE, Vol. 10, No. 4, 2015, p. 547-560.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Nanoscale stimulation of osteoblastogenesis from mesenchymal stem cells

T2 - nanotopography and nanokicking

AU - Pemberton, Gabriel D.

AU - Childs, Peter

AU - Reid, Stuart

AU - Nikukar, Habib

AU - Tsimbouri, P. Monica

AU - Gadegaard, Nikolaj

AU - Curtis, Adam S. G.

AU - Dalby, Matthew J.

PY - 2015

Y1 - 2015

N2 - Aim: Mesenchymal stem cells (MSCs) have large regenerative potential to replace damaged cells from several tissues along the mesodermal lineage. The potency of these cells promises to change the longer term prognosis for many degenerative conditions currently suffered by our aging population. We have endeavored to demonstrate our ability to induce osteoblatogenesis in MSCs using high-frequency (1000-5000 Hz) piezo-driven nanodisplacements (16-30 nm displacements) in a vertical direction. Materials & methods: Osteoblastogenesis has been determined by the upregulation of osteoblasic genes such as osteonectin (ONN), RUNX2 and Osterix, assessed via quantitative real-time PCR; the increase of osteocalcin (OCN) and osteopontin (OPN) at the protein level and the deposition of calcium phosphate determined by histological staining. Results: Intriguingly, we have observed a relationship between nanotopography and piezo-stimulated mechanotransduction and possibly see evidence of two differing osteogenic mechanisms at work. These data provide confidence in nanomechanotransduction for stem cell differentiation without dependence on soluble factors and complex chemistries. Conclusion: In the future it is envisaged that this technology may have beneficial therapeutic applications in the healthcare industry, for conditions whose overall phenotype maybe characterized by weak or damaged bones (e.g., osteoporosis and bone fractures), and which can benefit from having an increased number of osteoblastic cells in vivo.

AB - Aim: Mesenchymal stem cells (MSCs) have large regenerative potential to replace damaged cells from several tissues along the mesodermal lineage. The potency of these cells promises to change the longer term prognosis for many degenerative conditions currently suffered by our aging population. We have endeavored to demonstrate our ability to induce osteoblatogenesis in MSCs using high-frequency (1000-5000 Hz) piezo-driven nanodisplacements (16-30 nm displacements) in a vertical direction. Materials & methods: Osteoblastogenesis has been determined by the upregulation of osteoblasic genes such as osteonectin (ONN), RUNX2 and Osterix, assessed via quantitative real-time PCR; the increase of osteocalcin (OCN) and osteopontin (OPN) at the protein level and the deposition of calcium phosphate determined by histological staining. Results: Intriguingly, we have observed a relationship between nanotopography and piezo-stimulated mechanotransduction and possibly see evidence of two differing osteogenic mechanisms at work. These data provide confidence in nanomechanotransduction for stem cell differentiation without dependence on soluble factors and complex chemistries. Conclusion: In the future it is envisaged that this technology may have beneficial therapeutic applications in the healthcare industry, for conditions whose overall phenotype maybe characterized by weak or damaged bones (e.g., osteoporosis and bone fractures), and which can benefit from having an increased number of osteoblastic cells in vivo.

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KW - mesenchymal stem cells

KW - nanotopography

KW - nanovibration

KW - osteoblastogenesis

KW - piezo effect

KW - regenerative medicine

U2 - 10.2217/nnm.14.134

DO - 10.2217/nnm.14.134

M3 - Article

VL - 10

SP - 547

EP - 560

JO - NANOMEDICINE

JF - NANOMEDICINE

SN - 1743-5889

IS - 4

ER -

Pemberton GD, Childs P, Reid S, Nikukar H, Tsimbouri PM, Gadegaard N et al. Nanoscale stimulation of osteoblastogenesis from mesenchymal stem cells: nanotopography and nanokicking. NANOMEDICINE. 2015;10(4):547-560. https://doi.org/10.2217/nnm.14.134