Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats

Mohamed Ganal El-Din Ewees; Raha Orfali; Enas Ezzat Rateb; Hossam M. Hassan; Wael N. Hozzein; Dalal Hussien M. Alkhalfah; Haidy Tamer Abo Sree; Fatema El-Zahraa S. Abdel Rahman; Mostafa E. Rateb; Nesreen Ishak Mahmoud

doi:10.1016/j.etap.2023.104262

Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats

Mohamed Ganal El-Din Ewees
, Raha Orfali
, Enas Ezzat Rateb
, Hossam M. Hassan
, Wael N. Hozzein
, Dalal Hussien M. Alkhalfah
, Haidy Tamer Abo Sree
, Fatema El-Zahraa S. Abdel Rahman
, Mostafa E. Rateb
, Nesreen Ishak Mahmoud

Research output: Contribution to journal › Article › peer-review

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Abstract

Acute kidney injury (AKI) caused by Cis is considered one of the most severe adverse effects, which restricts its use and efficacy. This study seeks to examine the potential reno-protective impact of phenolic compound Hydroxytyrosol (HT) against Cis-induced AKI and the possible involvement of the mi-RNA25/Ox-LDL/NOX4 pathway elucidating the probable implicated molecular mechanisms. Forty rats were placed into 5 groups. Group I received saline only. Group II received Cis only. Group III, IV, and V received 20, 50, and 100 mg/kg b.w, of HT, respectively, with Cis delivery. NOX4, Ox-LDL, and gene expression of mi-RNA 25, TNF-α, and HO-1 in renal tissue were detected. HT showed reno-protective effect and significantly upregulated mi-RNA 25 and HO-1 as well as decreased the expression of NOX4, Ox-LDL, and TNF-α. In conclusion, HT may be promising in the fight against Cis-induced AKI through modulation of mi-RNA25/Ox-LDL/NOX4 pathway.

Original language	English
Article number	104262
Number of pages	9
Journal	Environmental Toxicology and Pharmacology
Volume	103
Early online date	10 Sept 2023
DOIs	https://doi.org/10.1016/j.etap.2023.104262
Publication status	Published - 31 Oct 2023
Externally published	Yes

Keywords

hydroxytyrosol
Mi-RNA 25
Ox-LDL
NOX4
HO-1
FOXO3a

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10.1016/j.etap.2023.104262

2023 09 06 Ewees et al Modulation acceptedAccepted author manuscript, 516 KBLicence: CC BY-NC-ND

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Ewees, M. G. E.-D., Orfali, R., Rateb, E. E., Hassan, H. M., Hozzein, W. N., Alkhalfah, D. H. M., Sree, H. T. A., Abdel Rahman, F. E.-Z. S., Rateb, M. E., & Mahmoud, N. I. (2023). Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats. Environmental Toxicology and Pharmacology, 103, Article 104262. https://doi.org/10.1016/j.etap.2023.104262

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title = "Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats",

abstract = "Acute kidney injury (AKI) caused by Cis is considered one of the most severe adverse effects, which restricts its use and efficacy. This study seeks to examine the potential reno-protective impact of phenolic compound Hydroxytyrosol (HT) against Cis-induced AKI and the possible involvement of the mi-RNA25/Ox-LDL/NOX4 pathway elucidating the probable implicated molecular mechanisms. Forty rats were placed into 5 groups. Group I received saline only. Group II received Cis only. Group III, IV, and V received 20, 50, and 100 mg/kg b.w, of HT, respectively, with Cis delivery. NOX4, Ox-LDL, and gene expression of mi-RNA 25, TNF-α, and HO-1 in renal tissue were detected. HT showed reno-protective effect and significantly upregulated mi-RNA 25 and HO-1 as well as decreased the expression of NOX4, Ox-LDL, and TNF-α. In conclusion, HT may be promising in the fight against Cis-induced AKI through modulation of mi-RNA25/Ox-LDL/NOX4 pathway.",

keywords = "hydroxytyrosol, Mi-RNA 25, Ox-LDL, NOX4, HO-1, FOXO3a",

author = "Ewees, \{Mohamed Ganal El-Din\} and Raha Orfali and Rateb, \{Enas Ezzat\} and Hassan, \{Hossam M.\} and Hozzein, \{Wael N.\} and Alkhalfah, \{Dalal Hussien M.\} and Sree, \{Haidy Tamer Abo\} and \{Abdel Rahman\}, \{Fatema El-Zahraa S.\} and Rateb, \{Mostafa E.\} and Mahmoud, \{Nesreen Ishak\}",

year = "2023",

month = oct,

day = "31",

doi = "10.1016/j.etap.2023.104262",

language = "English",

volume = "103",

journal = "Environmental Toxicology and Pharmacology",

issn = "1382-6689",

publisher = "Elsevier B.V.",

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Ewees, MGE-D, Orfali, R, Rateb, EE, Hassan, HM, Hozzein, WN, Alkhalfah, DHM, Sree, HTA, Abdel Rahman, FE-ZS, Rateb, ME & Mahmoud, NI 2023, 'Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats', Environmental Toxicology and Pharmacology, vol. 103, 104262. https://doi.org/10.1016/j.etap.2023.104262

Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats. / Ewees, Mohamed Ganal El-Din; Orfali, Raha; Rateb, Enas Ezzat et al.
In: Environmental Toxicology and Pharmacology, Vol. 103, 104262, 31.10.2023.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats

AU - Ewees, Mohamed Ganal El-Din

AU - Orfali, Raha

AU - Rateb, Enas Ezzat

AU - Hassan, Hossam M.

AU - Hozzein, Wael N.

AU - Alkhalfah, Dalal Hussien M.

AU - Sree, Haidy Tamer Abo

AU - Abdel Rahman, Fatema El-Zahraa S.

AU - Rateb, Mostafa E.

AU - Mahmoud, Nesreen Ishak

PY - 2023/10/31

Y1 - 2023/10/31

N2 - Acute kidney injury (AKI) caused by Cis is considered one of the most severe adverse effects, which restricts its use and efficacy. This study seeks to examine the potential reno-protective impact of phenolic compound Hydroxytyrosol (HT) against Cis-induced AKI and the possible involvement of the mi-RNA25/Ox-LDL/NOX4 pathway elucidating the probable implicated molecular mechanisms. Forty rats were placed into 5 groups. Group I received saline only. Group II received Cis only. Group III, IV, and V received 20, 50, and 100 mg/kg b.w, of HT, respectively, with Cis delivery. NOX4, Ox-LDL, and gene expression of mi-RNA 25, TNF-α, and HO-1 in renal tissue were detected. HT showed reno-protective effect and significantly upregulated mi-RNA 25 and HO-1 as well as decreased the expression of NOX4, Ox-LDL, and TNF-α. In conclusion, HT may be promising in the fight against Cis-induced AKI through modulation of mi-RNA25/Ox-LDL/NOX4 pathway.

AB - Acute kidney injury (AKI) caused by Cis is considered one of the most severe adverse effects, which restricts its use and efficacy. This study seeks to examine the potential reno-protective impact of phenolic compound Hydroxytyrosol (HT) against Cis-induced AKI and the possible involvement of the mi-RNA25/Ox-LDL/NOX4 pathway elucidating the probable implicated molecular mechanisms. Forty rats were placed into 5 groups. Group I received saline only. Group II received Cis only. Group III, IV, and V received 20, 50, and 100 mg/kg b.w, of HT, respectively, with Cis delivery. NOX4, Ox-LDL, and gene expression of mi-RNA 25, TNF-α, and HO-1 in renal tissue were detected. HT showed reno-protective effect and significantly upregulated mi-RNA 25 and HO-1 as well as decreased the expression of NOX4, Ox-LDL, and TNF-α. In conclusion, HT may be promising in the fight against Cis-induced AKI through modulation of mi-RNA25/Ox-LDL/NOX4 pathway.

KW - hydroxytyrosol

KW - Mi-RNA 25

KW - Ox-LDL

KW - NOX4

KW - HO-1

KW - FOXO3a

U2 - 10.1016/j.etap.2023.104262

DO - 10.1016/j.etap.2023.104262

M3 - Article

SN - 1382-6689

VL - 103

JO - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

M1 - 104262

ER -

Ewees MGED, Orfali R, Rateb EE, Hassan HM, Hozzein WN, Alkhalfah DHM et al. Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats. Environmental Toxicology and Pharmacology. 2023 Oct 31;103:104262. Epub 2023 Sept 10. doi: 10.1016/j.etap.2023.104262

Modulation of mi-RNA25/Ox-LDL/NOX4 signaling pathway by polyphenolic compound Hydroxytyrosol as a new avenue to alleviate cisplatin-induced acute kidney injury, a mechanistic study in rats

Abstract

Keywords

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