TY - JOUR
T1 - Metabolic profiling and biological potential of the marine sponge associated Nocardiopsis sp. UR67 along with docking studies
AU - Ibrahim, Alyaa Hatem
AU - Attia, Eman Zekry
AU - Hofny, Heba A.
AU - Alsenani, Faisal
AU - Zayed, Ahmed
AU - Rateb, Mostafa E.
AU - Abdelmohsen, Usama Ramadan
AU - Desoukey, Samar Yehia
AU - Fouad, Mostafa Ahmed
AU - Kamel, Mohamed Salah
PY - 2023
Y1 - 2023
N2 - This work was performed to dig into the phytochemical composition and bioactivities of Nocardiopsis sp. UR67 associated with the marine sponge Callyspongia sp. It was fermented in suspension and immobilised in calcium alginate bead cultures. The ethyl acetate extracts, afforded from the broth in each case named EG-49 and J-48g, respectively, revealed 16 chemical principles mostly belonging to polyketides, macrolides, and peptides. EG-49 and J-48g displayed anti-Candida albicans activity with IC50 values of 8.1 and 8.3 µg/mL, and a substantial cytotoxic effect against lung adenocarcinoma H1650 at IC50 12.6 and 13.7 µg/mL, respectively. However, only EG-49 exhibited a noteworthy anti-trypanosomal activity at 7.5 µg/mL. Molecular docking of the characterised compounds against Trypanosoma brucei trypanothione reductase demonstrated the highest binding models of griseochelin-methyl ester (9) and filipin-II (11), which drew considerable significance of the metabolites derived from Nocardiopsis sp. UR67 developing potential T. brucei trypanothione reductase inhibitors.
AB - This work was performed to dig into the phytochemical composition and bioactivities of Nocardiopsis sp. UR67 associated with the marine sponge Callyspongia sp. It was fermented in suspension and immobilised in calcium alginate bead cultures. The ethyl acetate extracts, afforded from the broth in each case named EG-49 and J-48g, respectively, revealed 16 chemical principles mostly belonging to polyketides, macrolides, and peptides. EG-49 and J-48g displayed anti-Candida albicans activity with IC50 values of 8.1 and 8.3 µg/mL, and a substantial cytotoxic effect against lung adenocarcinoma H1650 at IC50 12.6 and 13.7 µg/mL, respectively. However, only EG-49 exhibited a noteworthy anti-trypanosomal activity at 7.5 µg/mL. Molecular docking of the characterised compounds against Trypanosoma brucei trypanothione reductase demonstrated the highest binding models of griseochelin-methyl ester (9) and filipin-II (11), which drew considerable significance of the metabolites derived from Nocardiopsis sp. UR67 developing potential T. brucei trypanothione reductase inhibitors.
KW - Nocardiopsis
KW - marine sponges
KW - metabolomics
KW - anti-infective
KW - cytotoxicity
U2 - 10.1080/14786419.2022.2084396
DO - 10.1080/14786419.2022.2084396
M3 - Article
SN - 1478-6419
VL - 37
SP - 3531
EP - 3537
JO - Natural Product Research
JF - Natural Product Research
IS - 20
ER -
