Lower limb ischemic preconditioning combined with dietary nitrate supplementation does not influence time-trial performance in well-trained cyclists

Luke C. McIlvenna, David J. Muggeridge, Laura Forrest, Chris Monaghan, Luke Liddle, Mia C. Burleigh, Nicholas Sculthorpe, Bernadette O. Fernandez, Martin Feelisch, Chris Easton

Research output: Contribution to journalArticle

Abstract

Objectives
Dietary nitrate (NO3-) supplementation and ischaemic preconditioning (IPC) can independently improve exercise performance. The purpose of this study was to explore whether NO3- supplementation, ingested prior to an IPC protocol, could synergistically enhance parameters of exercise.

Design
Double-blind randomized crossover trial.

Methods
Ten competitive male cyclists (age 34 ± 6 years, body mass 78.9 ± 4.9 kg, V̇O2peak 55 ± 4 mL·kg·min-1) completed an incremental exercise test followed by three cycling trials comprising a square-wave submaximal component and a 16.1 km time-trial. Oxygen uptake (V̇O2) and muscle oxygenation kinetics were measured throughout. The baseline (BASE) trial was conducted without any dietary intervention or IPC. In the remaining two trials, participants received 3 × 5 min bouts of lower limb bilateral IPC prior to exercise. Participants ingested NO3--rich gel (NIT+IPC) 90 min prior to testing in one trial and a low NO3- placebo in the other (PLA+IPC). Plasma NO3- and nitrite (NO2-) were measured immediately before and after application of IPC.

Results
Plasma [NO3-] and [NO2-] were higher before and after IPC in NIT+IPC compared to BASE (P<0.001) but did not differ between BASE and PLA+IPC. There were no differences in V̇O2 kinetics or muscle oxygenation parameters between trials (all P>0.4). Performance in the time-trial was similar between trials (BASE 1343 ± 72 s, PLA+IPC 1350 ± 75 s, NIT+IPC 1346 ± 83 s, P=0.98).

Conclusions
Pre-exercise IPC did not improve sub-maximal exercise or performance measures, either alone or in combination with dietary NO3- supplementation.
Original languageEnglish
Pages (from-to)852-857
Number of pages6
JournalJournal of Science and Medicine in Sport
Volume22
Issue number7
Early online date26 Jan 2019
DOIs
Publication statusPublished - 31 Jul 2019

Fingerprint

Ischemic Preconditioning
Dietary Supplements
Nitrates
Lower Extremity
Nitrites
Exercise Test
Cross-Over Studies
Gels

Keywords

  • nitric oxide
  • blood flow
  • hyperaemia
  • nitrite
  • exercise

Cite this

@article{0b6b819a37844feb9abff758b61ed922,
title = "Lower limb ischemic preconditioning combined with dietary nitrate supplementation does not influence time-trial performance in well-trained cyclists",
abstract = "ObjectivesDietary nitrate (NO3-) supplementation and ischaemic preconditioning (IPC) can independently improve exercise performance. The purpose of this study was to explore whether NO3- supplementation, ingested prior to an IPC protocol, could synergistically enhance parameters of exercise. DesignDouble-blind randomized crossover trial.MethodsTen competitive male cyclists (age 34 ± 6 years, body mass 78.9 ± 4.9 kg, V̇O2peak 55 ± 4 mL·kg·min-1) completed an incremental exercise test followed by three cycling trials comprising a square-wave submaximal component and a 16.1 km time-trial. Oxygen uptake (V̇O2) and muscle oxygenation kinetics were measured throughout. The baseline (BASE) trial was conducted without any dietary intervention or IPC. In the remaining two trials, participants received 3 × 5 min bouts of lower limb bilateral IPC prior to exercise. Participants ingested NO3--rich gel (NIT+IPC) 90 min prior to testing in one trial and a low NO3- placebo in the other (PLA+IPC). Plasma NO3- and nitrite (NO2-) were measured immediately before and after application of IPC.ResultsPlasma [NO3-] and [NO2-] were higher before and after IPC in NIT+IPC compared to BASE (P<0.001) but did not differ between BASE and PLA+IPC. There were no differences in V̇O2 kinetics or muscle oxygenation parameters between trials (all P>0.4). Performance in the time-trial was similar between trials (BASE 1343 ± 72 s, PLA+IPC 1350 ± 75 s, NIT+IPC 1346 ± 83 s, P=0.98).ConclusionsPre-exercise IPC did not improve sub-maximal exercise or performance measures, either alone or in combination with dietary NO3- supplementation.",
keywords = "nitric oxide, blood flow, hyperaemia, nitrite, exercise",
author = "McIlvenna, {Luke C.} and Muggeridge, {David J.} and Laura Forrest and Chris Monaghan and Luke Liddle and Burleigh, {Mia C.} and Nicholas Sculthorpe and Fernandez, {Bernadette O.} and Martin Feelisch and Chris Easton",
year = "2019",
month = "7",
day = "31",
doi = "10.1016/j.jsams.2019.01.011",
language = "English",
volume = "22",
pages = "852--857",
journal = "Journal of Science and Medicine in Sport",
issn = "1440-2440",
publisher = "Elsevier B.V.",
number = "7",

}

TY - JOUR

T1 - Lower limb ischemic preconditioning combined with dietary nitrate supplementation does not influence time-trial performance in well-trained cyclists

AU - McIlvenna, Luke C.

AU - Muggeridge, David J.

AU - Forrest, Laura

AU - Monaghan, Chris

AU - Liddle, Luke

AU - Burleigh, Mia C.

AU - Sculthorpe, Nicholas

AU - Fernandez, Bernadette O.

AU - Feelisch, Martin

AU - Easton, Chris

PY - 2019/7/31

Y1 - 2019/7/31

N2 - ObjectivesDietary nitrate (NO3-) supplementation and ischaemic preconditioning (IPC) can independently improve exercise performance. The purpose of this study was to explore whether NO3- supplementation, ingested prior to an IPC protocol, could synergistically enhance parameters of exercise. DesignDouble-blind randomized crossover trial.MethodsTen competitive male cyclists (age 34 ± 6 years, body mass 78.9 ± 4.9 kg, V̇O2peak 55 ± 4 mL·kg·min-1) completed an incremental exercise test followed by three cycling trials comprising a square-wave submaximal component and a 16.1 km time-trial. Oxygen uptake (V̇O2) and muscle oxygenation kinetics were measured throughout. The baseline (BASE) trial was conducted without any dietary intervention or IPC. In the remaining two trials, participants received 3 × 5 min bouts of lower limb bilateral IPC prior to exercise. Participants ingested NO3--rich gel (NIT+IPC) 90 min prior to testing in one trial and a low NO3- placebo in the other (PLA+IPC). Plasma NO3- and nitrite (NO2-) were measured immediately before and after application of IPC.ResultsPlasma [NO3-] and [NO2-] were higher before and after IPC in NIT+IPC compared to BASE (P<0.001) but did not differ between BASE and PLA+IPC. There were no differences in V̇O2 kinetics or muscle oxygenation parameters between trials (all P>0.4). Performance in the time-trial was similar between trials (BASE 1343 ± 72 s, PLA+IPC 1350 ± 75 s, NIT+IPC 1346 ± 83 s, P=0.98).ConclusionsPre-exercise IPC did not improve sub-maximal exercise or performance measures, either alone or in combination with dietary NO3- supplementation.

AB - ObjectivesDietary nitrate (NO3-) supplementation and ischaemic preconditioning (IPC) can independently improve exercise performance. The purpose of this study was to explore whether NO3- supplementation, ingested prior to an IPC protocol, could synergistically enhance parameters of exercise. DesignDouble-blind randomized crossover trial.MethodsTen competitive male cyclists (age 34 ± 6 years, body mass 78.9 ± 4.9 kg, V̇O2peak 55 ± 4 mL·kg·min-1) completed an incremental exercise test followed by three cycling trials comprising a square-wave submaximal component and a 16.1 km time-trial. Oxygen uptake (V̇O2) and muscle oxygenation kinetics were measured throughout. The baseline (BASE) trial was conducted without any dietary intervention or IPC. In the remaining two trials, participants received 3 × 5 min bouts of lower limb bilateral IPC prior to exercise. Participants ingested NO3--rich gel (NIT+IPC) 90 min prior to testing in one trial and a low NO3- placebo in the other (PLA+IPC). Plasma NO3- and nitrite (NO2-) were measured immediately before and after application of IPC.ResultsPlasma [NO3-] and [NO2-] were higher before and after IPC in NIT+IPC compared to BASE (P<0.001) but did not differ between BASE and PLA+IPC. There were no differences in V̇O2 kinetics or muscle oxygenation parameters between trials (all P>0.4). Performance in the time-trial was similar between trials (BASE 1343 ± 72 s, PLA+IPC 1350 ± 75 s, NIT+IPC 1346 ± 83 s, P=0.98).ConclusionsPre-exercise IPC did not improve sub-maximal exercise or performance measures, either alone or in combination with dietary NO3- supplementation.

KW - nitric oxide

KW - blood flow

KW - hyperaemia

KW - nitrite

KW - exercise

U2 - 10.1016/j.jsams.2019.01.011

DO - 10.1016/j.jsams.2019.01.011

M3 - Article

VL - 22

SP - 852

EP - 857

JO - Journal of Science and Medicine in Sport

JF - Journal of Science and Medicine in Sport

SN - 1440-2440

IS - 7

ER -