Abstract
Switchable hydrophilicity solvents (SHS), also referred to as "smart" or "reversible" solvents, constitute a unique class of solvents capable of altering their hydrophilicity reversibly in response to external inducers such as CO2 or N2. This unique property has positioned SHS as a promising alternative to conventional solvents for lipid extraction from microalgae by leveraging their ability to undergo phase separation within a single solvent system. This feature not only minimizes environmental impact but also serves as a carbon capture source by consuming significant quantities of CO2 to transition from hydrophobic to hydrophilic states.
The SHS, N, N-Diisopropyl ethanolamine (DIPA) has demonstrated to be a viable alternative to hexane for lipid extraction of prokaryotic Blue-Green cyanobacteria. The process involves a microwave pretreatment which perforates the cell wall, enabling the hydrophobic solvent to access and extract the intracellular lipids. A maximum lipid yield of 27% dry weight was achieved using DIPA compared with hexane achieving only 18%. this work has identified a wild Blue-Green cyanobacterium strain found in the UK and sourced from a pond in the central belt of Scotland to contain sufficient quantities of lipids suitable for extraction. Characterisation of the methylated lipids through Gas Chromatography-Mass Spectrometry (GC-MS) reveals that DIPA has the ability to extract lipids suitable for biofuel applications.
This extraction process not only allows for solvent reuse through injection of N2, but also opens up the possibility of reinoculation of post-extracted biomass. This study has demonstrated a positive growth rate for 21 days, suggesting that DIPA facilitates a non-destructive, repetitive lipid extraction.
The SHS, N, N-Diisopropyl ethanolamine (DIPA) has demonstrated to be a viable alternative to hexane for lipid extraction of prokaryotic Blue-Green cyanobacteria. The process involves a microwave pretreatment which perforates the cell wall, enabling the hydrophobic solvent to access and extract the intracellular lipids. A maximum lipid yield of 27% dry weight was achieved using DIPA compared with hexane achieving only 18%. this work has identified a wild Blue-Green cyanobacterium strain found in the UK and sourced from a pond in the central belt of Scotland to contain sufficient quantities of lipids suitable for extraction. Characterisation of the methylated lipids through Gas Chromatography-Mass Spectrometry (GC-MS) reveals that DIPA has the ability to extract lipids suitable for biofuel applications.
This extraction process not only allows for solvent reuse through injection of N2, but also opens up the possibility of reinoculation of post-extracted biomass. This study has demonstrated a positive growth rate for 21 days, suggesting that DIPA facilitates a non-destructive, repetitive lipid extraction.
Original language | English |
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Publication status | Published - 10 Jun 2024 |
Event | International Conference on Algal Biomass, Biofuels and Bioproducts : AlgalBBB 2024 - Hilton Clearwater Beach, United States Duration: 10 Jun 2024 → 12 Jun 2024 |
Conference
Conference | International Conference on Algal Biomass, Biofuels and Bioproducts |
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Country/Territory | United States |
Period | 10/06/24 → 12/06/24 |