Sesbania grandiflora L. Poir leaves: a dietary supplement to alleviate type 2 diabetes through metabolic enzymes inhibition

Bathini Thissera, Rizliya Visvanathan, Mohammad A. Khanfar, M. Mallique Qader, Marwa H.A. Hassan, Hossam M. Hassan, Majed Bawazeer, Fathy A. Behery, Mohammed Yaseen, Ruvini Liyanage, Usama R. Abdelmohsen, Mostafa E. Rateb*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Sesbania grandiflora L. Poir is an edible medicinal plant widely distributed in Asian countries. One of its folk medicinal uses is the alleviation or treatment of Type 2 Diabetes mellitus (T2DM). A number of animal studies confirmed its use in treating T2DM; however, none of them explored the chemistry or the possible mechanism. This study aims to unveil the chemical profile of S. grandiflora through LC-HRMS dereplication analysis, followed by isolation, identification and quantification of the major secondary metabolites with potential α-amylase and α glucosidase inhibitory effect as the potential anti-diabetic mechanism. LC-HRMS chemical profiling of its leaves and twigs identified 32 metabolites. Bio-guided fractionation and HPLC purification led to the isolation of 14 major metabolites that were screened for their α-amylase and α-glucosidase inhibitory activities. For the first time, two terpenoids; vomifoliol (11) and loliolide (14) showed inhibitory effect against α-glucosidase with IC50 values of 64.5 and 388.48 µM, respectively. Quercetin (10) exhibited the highest α-glucosidase inhibition with IC50 value of 17.45 µM. Further, predicated molecular modelling studies demonstrated strong binding interactions between active compounds and enzyme-substrate binding pockets supporting the observed enzyme inhibitory activity. Interestingly, the quantitative analysis of the most potent inhibitors indicated their existence at a high percentage within S. grandiflora extract. Our findings suggested S. grandiflora is a useful dietary supplement to control postprandial blood glucose.
Original languageEnglish
Pages (from-to)282-299
Number of pages18
JournalSouth African Journal of Botany
Volume130
Early online date25 Jan 2020
DOIs
Publication statusE-pub ahead of print - 25 Jan 2020

Fingerprint

Sesbania grandiflora
glucosidases
enzyme inhibition
noninsulin-dependent diabetes mellitus
dietary supplements
amylases
inhibitory concentration 50
leaves
metabolites
enzyme substrates
terpenoids
quercetin
blood glucose
secondary metabolites
medicinal plants
quantitative analysis
fractionation
chemistry
extracts
enzymes

Keywords

  • Loliolide
  • Quercetin
  • Vomifoliol
  • Α-amylase
  • Α-glucosidase
  • Sesbania grandiflora

Cite this

Thissera, Bathini ; Visvanathan, Rizliya ; Khanfar, Mohammad A. ; Qader, M. Mallique ; Hassan, Marwa H.A. ; Hassan, Hossam M. ; Bawazeer, Majed ; Behery, Fathy A. ; Yaseen, Mohammed ; Liyanage, Ruvini ; Abdelmohsen, Usama R. ; Rateb, Mostafa E. / Sesbania grandiflora L. Poir leaves : a dietary supplement to alleviate type 2 diabetes through metabolic enzymes inhibition. In: South African Journal of Botany . 2020 ; Vol. 130. pp. 282-299.
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abstract = "Sesbania grandiflora L. Poir is an edible medicinal plant widely distributed in Asian countries. One of its folk medicinal uses is the alleviation or treatment of Type 2 Diabetes mellitus (T2DM). A number of animal studies confirmed its use in treating T2DM; however, none of them explored the chemistry or the possible mechanism. This study aims to unveil the chemical profile of S. grandiflora through LC-HRMS dereplication analysis, followed by isolation, identification and quantification of the major secondary metabolites with potential α-amylase and α glucosidase inhibitory effect as the potential anti-diabetic mechanism. LC-HRMS chemical profiling of its leaves and twigs identified 32 metabolites. Bio-guided fractionation and HPLC purification led to the isolation of 14 major metabolites that were screened for their α-amylase and α-glucosidase inhibitory activities. For the first time, two terpenoids; vomifoliol (11) and loliolide (14) showed inhibitory effect against α-glucosidase with IC50 values of 64.5 and 388.48 µM, respectively. Quercetin (10) exhibited the highest α-glucosidase inhibition with IC50 value of 17.45 µM. Further, predicated molecular modelling studies demonstrated strong binding interactions between active compounds and enzyme-substrate binding pockets supporting the observed enzyme inhibitory activity. Interestingly, the quantitative analysis of the most potent inhibitors indicated their existence at a high percentage within S. grandiflora extract. Our findings suggested S. grandiflora is a useful dietary supplement to control postprandial blood glucose.",
keywords = "Loliolide, Quercetin, Vomifoliol, Α-amylase, Α-glucosidase, Sesbania grandiflora",
author = "Bathini Thissera and Rizliya Visvanathan and Khanfar, {Mohammad A.} and Qader, {M. Mallique} and Hassan, {Marwa H.A.} and Hassan, {Hossam M.} and Majed Bawazeer and Behery, {Fathy A.} and Mohammed Yaseen and Ruvini Liyanage and Abdelmohsen, {Usama R.} and Rateb, {Mostafa E.}",
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Thissera, B, Visvanathan, R, Khanfar, MA, Qader, MM, Hassan, MHA, Hassan, HM, Bawazeer, M, Behery, FA, Yaseen, M, Liyanage, R, Abdelmohsen, UR & Rateb, ME 2020, 'Sesbania grandiflora L. Poir leaves: a dietary supplement to alleviate type 2 diabetes through metabolic enzymes inhibition', South African Journal of Botany , vol. 130, pp. 282-299. https://doi.org/10.1016/j.sajb.2020.01.011

Sesbania grandiflora L. Poir leaves : a dietary supplement to alleviate type 2 diabetes through metabolic enzymes inhibition. / Thissera, Bathini; Visvanathan, Rizliya; Khanfar, Mohammad A.; Qader, M. Mallique; Hassan, Marwa H.A.; Hassan, Hossam M.; Bawazeer, Majed; Behery, Fathy A.; Yaseen, Mohammed; Liyanage, Ruvini; Abdelmohsen, Usama R.; Rateb, Mostafa E.

In: South African Journal of Botany , Vol. 130, 31.05.2020, p. 282-299.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sesbania grandiflora L. Poir leaves

T2 - a dietary supplement to alleviate type 2 diabetes through metabolic enzymes inhibition

AU - Thissera, Bathini

AU - Visvanathan, Rizliya

AU - Khanfar, Mohammad A.

AU - Qader, M. Mallique

AU - Hassan, Marwa H.A.

AU - Hassan, Hossam M.

AU - Bawazeer, Majed

AU - Behery, Fathy A.

AU - Yaseen, Mohammed

AU - Liyanage, Ruvini

AU - Abdelmohsen, Usama R.

AU - Rateb, Mostafa E.

PY - 2020/1/25

Y1 - 2020/1/25

N2 - Sesbania grandiflora L. Poir is an edible medicinal plant widely distributed in Asian countries. One of its folk medicinal uses is the alleviation or treatment of Type 2 Diabetes mellitus (T2DM). A number of animal studies confirmed its use in treating T2DM; however, none of them explored the chemistry or the possible mechanism. This study aims to unveil the chemical profile of S. grandiflora through LC-HRMS dereplication analysis, followed by isolation, identification and quantification of the major secondary metabolites with potential α-amylase and α glucosidase inhibitory effect as the potential anti-diabetic mechanism. LC-HRMS chemical profiling of its leaves and twigs identified 32 metabolites. Bio-guided fractionation and HPLC purification led to the isolation of 14 major metabolites that were screened for their α-amylase and α-glucosidase inhibitory activities. For the first time, two terpenoids; vomifoliol (11) and loliolide (14) showed inhibitory effect against α-glucosidase with IC50 values of 64.5 and 388.48 µM, respectively. Quercetin (10) exhibited the highest α-glucosidase inhibition with IC50 value of 17.45 µM. Further, predicated molecular modelling studies demonstrated strong binding interactions between active compounds and enzyme-substrate binding pockets supporting the observed enzyme inhibitory activity. Interestingly, the quantitative analysis of the most potent inhibitors indicated their existence at a high percentage within S. grandiflora extract. Our findings suggested S. grandiflora is a useful dietary supplement to control postprandial blood glucose.

AB - Sesbania grandiflora L. Poir is an edible medicinal plant widely distributed in Asian countries. One of its folk medicinal uses is the alleviation or treatment of Type 2 Diabetes mellitus (T2DM). A number of animal studies confirmed its use in treating T2DM; however, none of them explored the chemistry or the possible mechanism. This study aims to unveil the chemical profile of S. grandiflora through LC-HRMS dereplication analysis, followed by isolation, identification and quantification of the major secondary metabolites with potential α-amylase and α glucosidase inhibitory effect as the potential anti-diabetic mechanism. LC-HRMS chemical profiling of its leaves and twigs identified 32 metabolites. Bio-guided fractionation and HPLC purification led to the isolation of 14 major metabolites that were screened for their α-amylase and α-glucosidase inhibitory activities. For the first time, two terpenoids; vomifoliol (11) and loliolide (14) showed inhibitory effect against α-glucosidase with IC50 values of 64.5 and 388.48 µM, respectively. Quercetin (10) exhibited the highest α-glucosidase inhibition with IC50 value of 17.45 µM. Further, predicated molecular modelling studies demonstrated strong binding interactions between active compounds and enzyme-substrate binding pockets supporting the observed enzyme inhibitory activity. Interestingly, the quantitative analysis of the most potent inhibitors indicated their existence at a high percentage within S. grandiflora extract. Our findings suggested S. grandiflora is a useful dietary supplement to control postprandial blood glucose.

KW - Loliolide

KW - Quercetin

KW - Vomifoliol

KW - Α-amylase

KW - Α-glucosidase

KW - Sesbania grandiflora

U2 - 10.1016/j.sajb.2020.01.011

DO - 10.1016/j.sajb.2020.01.011

M3 - Article

VL - 130

SP - 282

EP - 299

JO - South African Journal of Botany

JF - South African Journal of Botany

SN - 0254-6299

ER -