Investigation of albumin properties in patients with chronic renal failure

Anna Pieniazek, Joanna Brzeszczynska, Ilona Kruszynska, Krzysztof Gwozdzinski

Research output: Contribution to journalArticle

Abstract

The aim of this study was the investigation of HSA properties and its structural changes after modification induced in vivo among patients with CRF who underwent haemodialysis. Application of different fluorescent dyes allowed the investigation of different regions of albumin molecule using ANS, bis-ANS, piren, piren maleimide and fluorescein isothiocyanate. As markers of oxidative modification, the total protein thiol, carbonyls, glycosylated plasma proteins and hydroperoxide were estimated in plasma. Additionally, this study investigated plasma viscosity and total antioxidant capacity (TAC) of the plasma. Results show that haemodialysis provoked significant changes in conformational properties of plasma albumin, which resulted in the loss of its biological functions. These findings suggest that oxidative stress and glycation of proteins in plasma are developed during haemodialysis. The results depict that one of the features of uraemia is the presence of signs of oxidative stress before haemodialysis. Nevertheless, oxidative stress and glycation of proteins in plasma are exacerbated during haemodialysis and are a complex process.
Original languageEnglish
Pages (from-to)1008-1018
Number of pages11
JournalFree Radical Research
Volume43
Issue number10
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Chronic Kidney Failure
Renal Dialysis
Albumins
Oxidative Stress
Heat-Shock Proteins
Uremia
Fluorescent Dyes
Sulfhydryl Compounds
Viscosity
Serum Albumin
Hydrogen Peroxide
Blood Proteins
Antioxidants
Proteins

Keywords

  • Albumin
  • Chronic renal failure
  • Haemodialysis
  • Oxidative stress
  • Glycosylation

Cite this

Pieniazek, Anna ; Brzeszczynska, Joanna ; Kruszynska, Ilona ; Gwozdzinski, Krzysztof. / Investigation of albumin properties in patients with chronic renal failure. In: Free Radical Research. 2009 ; Vol. 43, No. 10. pp. 1008-1018.
@article{fc103a852735475db7f5e2bfaba71702,
title = "Investigation of albumin properties in patients with chronic renal failure",
abstract = "The aim of this study was the investigation of HSA properties and its structural changes after modification induced in vivo among patients with CRF who underwent haemodialysis. Application of different fluorescent dyes allowed the investigation of different regions of albumin molecule using ANS, bis-ANS, piren, piren maleimide and fluorescein isothiocyanate. As markers of oxidative modification, the total protein thiol, carbonyls, glycosylated plasma proteins and hydroperoxide were estimated in plasma. Additionally, this study investigated plasma viscosity and total antioxidant capacity (TAC) of the plasma. Results show that haemodialysis provoked significant changes in conformational properties of plasma albumin, which resulted in the loss of its biological functions. These findings suggest that oxidative stress and glycation of proteins in plasma are developed during haemodialysis. The results depict that one of the features of uraemia is the presence of signs of oxidative stress before haemodialysis. Nevertheless, oxidative stress and glycation of proteins in plasma are exacerbated during haemodialysis and are a complex process.",
keywords = "Albumin, Chronic renal failure, Haemodialysis, Oxidative stress, Glycosylation",
author = "Anna Pieniazek and Joanna Brzeszczynska and Ilona Kruszynska and Krzysztof Gwozdzinski",
year = "2009",
doi = "10.1080/10715760903165003",
language = "English",
volume = "43",
pages = "1008--1018",
journal = "Free Radical Research",
issn = "1071-5762",
publisher = "Routledge Taylor & Francis Group",
number = "10",

}

Investigation of albumin properties in patients with chronic renal failure. / Pieniazek, Anna; Brzeszczynska, Joanna; Kruszynska, Ilona; Gwozdzinski, Krzysztof.

In: Free Radical Research, Vol. 43, No. 10, 2009, p. 1008-1018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Investigation of albumin properties in patients with chronic renal failure

AU - Pieniazek, Anna

AU - Brzeszczynska, Joanna

AU - Kruszynska, Ilona

AU - Gwozdzinski, Krzysztof

PY - 2009

Y1 - 2009

N2 - The aim of this study was the investigation of HSA properties and its structural changes after modification induced in vivo among patients with CRF who underwent haemodialysis. Application of different fluorescent dyes allowed the investigation of different regions of albumin molecule using ANS, bis-ANS, piren, piren maleimide and fluorescein isothiocyanate. As markers of oxidative modification, the total protein thiol, carbonyls, glycosylated plasma proteins and hydroperoxide were estimated in plasma. Additionally, this study investigated plasma viscosity and total antioxidant capacity (TAC) of the plasma. Results show that haemodialysis provoked significant changes in conformational properties of plasma albumin, which resulted in the loss of its biological functions. These findings suggest that oxidative stress and glycation of proteins in plasma are developed during haemodialysis. The results depict that one of the features of uraemia is the presence of signs of oxidative stress before haemodialysis. Nevertheless, oxidative stress and glycation of proteins in plasma are exacerbated during haemodialysis and are a complex process.

AB - The aim of this study was the investigation of HSA properties and its structural changes after modification induced in vivo among patients with CRF who underwent haemodialysis. Application of different fluorescent dyes allowed the investigation of different regions of albumin molecule using ANS, bis-ANS, piren, piren maleimide and fluorescein isothiocyanate. As markers of oxidative modification, the total protein thiol, carbonyls, glycosylated plasma proteins and hydroperoxide were estimated in plasma. Additionally, this study investigated plasma viscosity and total antioxidant capacity (TAC) of the plasma. Results show that haemodialysis provoked significant changes in conformational properties of plasma albumin, which resulted in the loss of its biological functions. These findings suggest that oxidative stress and glycation of proteins in plasma are developed during haemodialysis. The results depict that one of the features of uraemia is the presence of signs of oxidative stress before haemodialysis. Nevertheless, oxidative stress and glycation of proteins in plasma are exacerbated during haemodialysis and are a complex process.

KW - Albumin

KW - Chronic renal failure

KW - Haemodialysis

KW - Oxidative stress

KW - Glycosylation

U2 - 10.1080/10715760903165003

DO - 10.1080/10715760903165003

M3 - Article

VL - 43

SP - 1008

EP - 1018

JO - Free Radical Research

JF - Free Radical Research

SN - 1071-5762

IS - 10

ER -