Activities per year
Expression of matriptase, PAR2 and ENaC was evaluated in lung epithelial cell lines (A549 and BEAS-2B) by immunofluorescence. Western blot was used to look at regulation of these markers by transforming growth factor β (TGFβ) and hypoxia inducer, dimethyloxalylglycine (DMOG).
A549 cells expressed all three markers with TGFβ increasing matriptase while DMOG decreased ENaC. There was no effect on PAR2. BEAS-2B cells expressed PAR2 and ENaC but not matriptase.
These data provide a basis for future functional studies looking at PAR2/ matriptase interaction and regulation of ENaC in lung epithelia, which may give insight into a potential role for this axis in COPD pathogenesis.
1. Vuagniaux G, Vallet V, Jaeger NF, Hummler E, Rossier BC. Synergistic Activation of ENaC by Three Membrane-bound Channel-activating Serine Proteases (mCAP1, mCAP2, and mCAP3) and Serum- and Glucocorticoidregulated Kinase (Sgk1) in Xenopus Oocytes. J Gen Physiol. 2002;
2. Zhao R, Liang X, Zhao M, Liu SL, Huang Y, Idell S, et al. Correlation of apical fluid-regulating channel proteins with lung function in human COPD lungs. PLoS One. 2014;
|Number of pages||1|
|Journal||Irish Journal of Medical Science|
|Issue number||Supplement 8|
|Publication status||Published - 8 Oct 2018|
|Event||Irish Thoracic Society Annual Scientific Meeting - Hastings Europa Hotel, Belfast, United Kingdom|
Duration: 23 Nov 2018 → 24 Nov 2018
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John Lockhart (Member)28 Feb 2017 → …
Activity: Membership › Membership of committee
John Lockhart, Gary Litherland, Anne Crilly, Carl Goodyear, Andrew MacKenzie, Iain McLellan, Andisheh Bakhshi, Lynette Dunning, Robin Freeburn, William MacKay, Craig Williams, Kirsty McCallum, Mariarca Bailo, Kimberly Black, Bryn Short, Mark Thomas & Fawziye Tarhini
1 Media contribution
1 item of Media coverage
Lorraine Martin (Recipient), Lorcan McGarvey (Recipient), Fionnuala Lundy (Recipient), Litherland, Gary (Recipient), Lockhart, John (Recipient), Crilly, Anne (Recipient), Carl Goodyear (Recipient), Keith Thornbury (Recipient), Mark Hollywood (Recipient), Gerard Sergeant (Recipient) & Noel McHale (Recipient), 22 Feb 2018
Prize: National/international honour