Activities per year
Abstract
Expression of matriptase, PAR2 and ENaC was evaluated in lung epithelial cell lines (A549 and BEAS-2B) by immunofluorescence. Western blot was used to look at regulation of these markers by transforming growth factor β (TGFβ) and hypoxia inducer, dimethyloxalylglycine (DMOG).
A549 cells expressed all three markers with TGFβ increasing matriptase while DMOG decreased ENaC. There was no effect on PAR2. BEAS-2B cells expressed PAR2 and ENaC but not matriptase.
These data provide a basis for future functional studies looking at PAR2/ matriptase interaction and regulation of ENaC in lung epithelia, which may give insight into a potential role for this axis in COPD pathogenesis.
1. Vuagniaux G, Vallet V, Jaeger NF, Hummler E, Rossier BC. Synergistic Activation of ENaC by Three Membrane-bound Channel-activating Serine Proteases (mCAP1, mCAP2, and mCAP3) and Serum- and Glucocorticoidregulated Kinase (Sgk1) in Xenopus Oocytes. J Gen Physiol. 2002;
2. Zhao R, Liang X, Zhao M, Liu SL, Huang Y, Idell S, et al. Correlation of apical fluid-regulating channel proteins with lung function in human COPD lungs. PLoS One. 2014;
Original language | English |
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Pages (from-to) | S249 |
Number of pages | 1 |
Journal | Irish Journal of Medical Science |
Volume | 187 |
Issue number | Supplement 8 |
DOIs | |
Publication status | Published - 8 Oct 2018 |
Event | Irish Thoracic Society Annual Scientific Meeting - Hastings Europa Hotel, Belfast, United Kingdom Duration: 23 Nov 2018 → 24 Nov 2018 |
Keywords
- PAR2
- COPD
- BREATH
- interreg va
- seupb
- matriptase
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Dive into the research topics of 'Investigating the matriptase-PAR2-ENaC axis in lung epithelial cells: potential role in COPD pathogenesis'. Together they form a unique fingerprint.Activities
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BREATH Ayrshire Stakeholder Event
John Lockhart (Speaker), Gary Litherland (Speaker), Anur Guhan (Speaker), Lorcan McGarvey (Speaker), Keith Thornbury (Speaker) & Iain McLellan (Speaker)
22 Aug 2018Activity: Talk or presentation › Oral presentation
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Cross Party Group on Lung Health (Scottish Parliament) (External organisation)
John Lockhart (Member)
28 Feb 2017 → …Activity: Membership › Membership of committee
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BREATH Dumfries and Galloway Launch Event
Anne Crilly (Participant), Gary Litherland (Speaker) & John Lockhart (Speaker)
Aug 2017Activity: Other › Types of Public engagement and outreach - Public lecture/debate/seminar
Press / Media
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BREATH Schools Engagement in Dumfries & Galloway
John Lockhart, Anne Crilly, Gary Litherland, Kimberly Black, Kirsty McCallum & Mariarca Bailo
6/12/18
1 item of Media coverage
Press/Media: Public Engagement Activities
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BREATH highlighted in Scottish Parliament Debate
John Lockhart, Gary Litherland, Anne Crilly, Carl Goodyear, Andrew MacKenzie, Iain McLellan, Andisheh Bakhshi, Lynette Dunning, Robin Freeburn, William MacKay, Craig Williams, Kirsty McCallum, Mariarca Bailo, Kimberly Black, Bryn Short, Mark Thomas & Fawziye Tarhini
7/11/18
1 Media contribution
Press/Media: Other
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Scientists honoured for their bid to ease lung disease misery
John Lockhart, Gary Litherland, Anne Crilly, Carl Goodyear, Lorraine Martin, Keith Thornbury, Mark Hollywood, Gerard Sergeant, Fionnuala Lundy & Lorcan McGarvey
9/03/18
1 item of Media coverage
Press/Media: Research
Prizes
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Asthma/COPD Project of the Year, 19th Northern Ireland Healthcare Awards 2018
Martin, Lorraine (Recipient), McGarvey, Lorcan (Recipient), Lundy, Fionnuala (Recipient), Litherland, Gary (Recipient), Lockhart, John (Recipient), Crilly, Anne (Recipient), Goodyear, Carl (Recipient), Thornbury, Keith (Recipient), Hollywood, Mark (Recipient), Sergeant, Gerard (Recipient) & McHale, Noel (Recipient), 22 Feb 2018
Prize: National/international honour
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Northern Ireland Healthcare Awards
Lockhart, John (Recipient), 22 Feb 2018
Prize: Prize (including medals and awards)