Internal labelling of oligonucleotide probes by Diels Alder cycloaddition

Duncan Graham, Antonio Grondin, Callum McHugh, Ljiljana Fruk, W. Ewen Smith

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36 Citations (Scopus)

Abstract

A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diels–Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2′-deoxyuridine modified at the 5-position with a spaced furan. This is a new approach to internal oligonucleotide chemistry that opens up a large range of possibilities for further conjugation.

Synthesis of a furan modified deoxyuridine phosphoramidite that was used to add fluorescent maleimides to the middle of an oligonucleotide is reported.
Original languageEnglish
Pages (from-to)4785-4788
Number of pages4
JournalTetrahedron Letters
Volume43
Issue number27
DOIs
Publication statusPublished - 2002
Externally publishedYes

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