Insulin regulates the expression of the GLUT5 transporter in L6 skeletal muscle cells

Eric Hajduch, Gary Litherland, Sophie Turban, Edith Brot-Laroche, Harinder S. Hundal

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Skeletal muscle, a primary insulin target tissue, expresses the GLUT5 fructose transporter. Although insulin has no acute effect on GLUT5 expression and function in muscle, we show here that long-term (24 h) insulin treatment of L6 muscle cells induces a dose-dependent increase in GLUT5 protein (by up to two-fold), leading to a concomitant increase in fructose uptake. The increase in GLUT5 expression and function was suppressed by inhibitors of gene transcription and protein synthesis, suggesting that insulin promotes de novo carrier synthesis. Transfection of the GLUT5 gene promoter fused to luciferase into L6 cells revealed that insulin induced a 1.8-fold increase in GLUT5 promoter activity. Our findings indicate that insulin is capable of increasing the abundance and functional activity of GLUT5 in skeletal muscle cells and that this is most likely mediated via activation of the GLUT5 promoter.
Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalFEBS Letters
Volume549
Issue number1-3
DOIs
Publication statusPublished - 14 Aug 2003
Externally publishedYes

Keywords

  • Fructose
  • Glucose
  • GLUT5 promoter
  • Luciferase

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