In vivo magnetic resonance imaging features of spinal muscles in the ovine model

Stephanie Valentin, Theresia Licka, Annika Essigbeck, James Elliott

Research output: Contribution to journalArticle

Abstract

Background
Muscle fatty infiltration (MFI) has been identified in patients with spinal pain using magnetic resonance imaging (MRI). Even though sheep are a commonly used animal model for the human spine, comparative sheep MFI data from MRI is not available. Determining MFI in sheep spinal muscles using acquisition protocols commonly used in man will identify the applicability of this approach in future sheep model studies, such that the effects of spinal interventions on muscle can be assessed prior to their use in a human (clinical) population.

Objective
To quantify ovine lumbar spine MFI using three-dimensional two-point Dixon and T1-weighted sequences.

Methods
T1-weighted and Dixon lumbar spine axial sequences were collected in 14 healthy Austrian mountain sheep using a 1.5-T MRI. At each vertebrae, the region of interest of psoas major and minor (PS), multifidus (M), and longissimus (L) were identified. To determine MFI from the T1-weighted images, the mean pixel intensity (MPI) was calculated as a percentage of subcutaneous or intermuscular fat. For the Dixon images, fat sequence MPI was calculated as a percentage of the summed fat and water sequence MPIs. Spinal degeneration was graded and correlated to MFI. Dixon MFI was compared to T1-weighted MFI obtained from subcutaneous and intermuscular fat.

Results
For every muscle, T1-weighted MFI calculated using subcutaneous fat scored significantly lower than Dixon MFI and T1-weighted MFI calculated using intermuscular fat (p < 0.001). There were no significant MFI differences between T1-weighted images calculated using intermuscular fat and Dixon images for M and L (p > 0.05), although significant differences were found for PS.

Conclusion
In sheep, Dixon sequences provide an acceptable comparison to T1-weighted sequences for lumbar extensor MFI based on intermuscular fat. However, compared to the human literature, ovine lumbar musculature contains greater MFI, making interspecies comparisons more complex.
Original languageEnglish
Pages (from-to)1-9
JournalJournal of Orthopaedic Translation
Volume6
Early online date23 Oct 2015
DOIs
Publication statusPublished - 1 Jul 2016
Externally publishedYes

Fingerprint

Sheep
Magnetic Resonance Imaging
Muscles
Fats
Spine
Subcutaneous Fat
Paraspinal Muscles
Animal Models
Pain
Water
Population

Keywords

  • animal models
  • lumbar spine
  • magnetic resonance imaging
  • muscle fatty infiltration
  • ovine

Cite this

Valentin, Stephanie ; Licka, Theresia ; Essigbeck, Annika ; Elliott, James. / In vivo magnetic resonance imaging features of spinal muscles in the ovine model. In: Journal of Orthopaedic Translation. 2016 ; Vol. 6. pp. 1-9.
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abstract = "BackgroundMuscle fatty infiltration (MFI) has been identified in patients with spinal pain using magnetic resonance imaging (MRI). Even though sheep are a commonly used animal model for the human spine, comparative sheep MFI data from MRI is not available. Determining MFI in sheep spinal muscles using acquisition protocols commonly used in man will identify the applicability of this approach in future sheep model studies, such that the effects of spinal interventions on muscle can be assessed prior to their use in a human (clinical) population.ObjectiveTo quantify ovine lumbar spine MFI using three-dimensional two-point Dixon and T1-weighted sequences.MethodsT1-weighted and Dixon lumbar spine axial sequences were collected in 14 healthy Austrian mountain sheep using a 1.5-T MRI. At each vertebrae, the region of interest of psoas major and minor (PS), multifidus (M), and longissimus (L) were identified. To determine MFI from the T1-weighted images, the mean pixel intensity (MPI) was calculated as a percentage of subcutaneous or intermuscular fat. For the Dixon images, fat sequence MPI was calculated as a percentage of the summed fat and water sequence MPIs. Spinal degeneration was graded and correlated to MFI. Dixon MFI was compared to T1-weighted MFI obtained from subcutaneous and intermuscular fat.ResultsFor every muscle, T1-weighted MFI calculated using subcutaneous fat scored significantly lower than Dixon MFI and T1-weighted MFI calculated using intermuscular fat (p < 0.001). There were no significant MFI differences between T1-weighted images calculated using intermuscular fat and Dixon images for M and L (p > 0.05), although significant differences were found for PS.ConclusionIn sheep, Dixon sequences provide an acceptable comparison to T1-weighted sequences for lumbar extensor MFI based on intermuscular fat. However, compared to the human literature, ovine lumbar musculature contains greater MFI, making interspecies comparisons more complex.",
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In vivo magnetic resonance imaging features of spinal muscles in the ovine model. / Valentin, Stephanie; Licka, Theresia; Essigbeck, Annika; Elliott, James.

In: Journal of Orthopaedic Translation, Vol. 6, 01.07.2016, p. 1-9.

Research output: Contribution to journalArticle

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T1 - In vivo magnetic resonance imaging features of spinal muscles in the ovine model

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AU - Elliott, James

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N2 - BackgroundMuscle fatty infiltration (MFI) has been identified in patients with spinal pain using magnetic resonance imaging (MRI). Even though sheep are a commonly used animal model for the human spine, comparative sheep MFI data from MRI is not available. Determining MFI in sheep spinal muscles using acquisition protocols commonly used in man will identify the applicability of this approach in future sheep model studies, such that the effects of spinal interventions on muscle can be assessed prior to their use in a human (clinical) population.ObjectiveTo quantify ovine lumbar spine MFI using three-dimensional two-point Dixon and T1-weighted sequences.MethodsT1-weighted and Dixon lumbar spine axial sequences were collected in 14 healthy Austrian mountain sheep using a 1.5-T MRI. At each vertebrae, the region of interest of psoas major and minor (PS), multifidus (M), and longissimus (L) were identified. To determine MFI from the T1-weighted images, the mean pixel intensity (MPI) was calculated as a percentage of subcutaneous or intermuscular fat. For the Dixon images, fat sequence MPI was calculated as a percentage of the summed fat and water sequence MPIs. Spinal degeneration was graded and correlated to MFI. Dixon MFI was compared to T1-weighted MFI obtained from subcutaneous and intermuscular fat.ResultsFor every muscle, T1-weighted MFI calculated using subcutaneous fat scored significantly lower than Dixon MFI and T1-weighted MFI calculated using intermuscular fat (p < 0.001). There were no significant MFI differences between T1-weighted images calculated using intermuscular fat and Dixon images for M and L (p > 0.05), although significant differences were found for PS.ConclusionIn sheep, Dixon sequences provide an acceptable comparison to T1-weighted sequences for lumbar extensor MFI based on intermuscular fat. However, compared to the human literature, ovine lumbar musculature contains greater MFI, making interspecies comparisons more complex.

AB - BackgroundMuscle fatty infiltration (MFI) has been identified in patients with spinal pain using magnetic resonance imaging (MRI). Even though sheep are a commonly used animal model for the human spine, comparative sheep MFI data from MRI is not available. Determining MFI in sheep spinal muscles using acquisition protocols commonly used in man will identify the applicability of this approach in future sheep model studies, such that the effects of spinal interventions on muscle can be assessed prior to their use in a human (clinical) population.ObjectiveTo quantify ovine lumbar spine MFI using three-dimensional two-point Dixon and T1-weighted sequences.MethodsT1-weighted and Dixon lumbar spine axial sequences were collected in 14 healthy Austrian mountain sheep using a 1.5-T MRI. At each vertebrae, the region of interest of psoas major and minor (PS), multifidus (M), and longissimus (L) were identified. To determine MFI from the T1-weighted images, the mean pixel intensity (MPI) was calculated as a percentage of subcutaneous or intermuscular fat. For the Dixon images, fat sequence MPI was calculated as a percentage of the summed fat and water sequence MPIs. Spinal degeneration was graded and correlated to MFI. Dixon MFI was compared to T1-weighted MFI obtained from subcutaneous and intermuscular fat.ResultsFor every muscle, T1-weighted MFI calculated using subcutaneous fat scored significantly lower than Dixon MFI and T1-weighted MFI calculated using intermuscular fat (p < 0.001). There were no significant MFI differences between T1-weighted images calculated using intermuscular fat and Dixon images for M and L (p > 0.05), although significant differences were found for PS.ConclusionIn sheep, Dixon sequences provide an acceptable comparison to T1-weighted sequences for lumbar extensor MFI based on intermuscular fat. However, compared to the human literature, ovine lumbar musculature contains greater MFI, making interspecies comparisons more complex.

KW - animal models

KW - lumbar spine

KW - magnetic resonance imaging

KW - muscle fatty infiltration

KW - ovine

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