Implications of antimicrobial combinations in complex wound biofilms containing fungi

Eleanor M Townsend; Leighann Sherry; Ryan Kean; Donald Hansom; William G Mackay; Craig Williams; John Butcher; Gordon Ramage

doi:10.1128/AAC.00672-17

Implications of antimicrobial combinations in complex wound biofilms containing fungi

Eleanor M Townsend
, Leighann Sherry
, Ryan Kean
, Donald Hansom
, William G Mackay
, Craig Williams
, John Butcher
, Gordon Ramage

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

171 Downloads (Pure)

Abstract

Diabetic foot ulcer treatment currently focuses on targeting bacterial biofilms, while dismissing fungi. To investigate this we used an in vitro biofilm model containing bacteria and fungi, reflective of the wound environment, to test the impact of antimicrobials. Here we showed that while mono-treatment approaches influenced biofilm composition it had no discernible effect on overall quantity. Only by combining bacterial and fungal specific antibiotics were we able to decrease the biofilm bioburden, irrespective of composition.

Original language	English
Article number	e00672-17
Number of pages	5
Journal	Antimicrobial Agents and Chemotherapy
Volume	61
Issue number	9
Early online date	10 Jul 2017
DOIs	https://doi.org/10.1128/AAC.00672-17
Publication status	Published - 1 Sept 2017

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Biofilm
Polymicrobial
Chronic wound
Antimicrobial
Candida

Access to Document

10.1128/AAC.00672-17

2017 07 03 Townsend et al Implications finalAccepted author manuscript, 651 KB

Cite this

@article{aee8a7aaa7424456805b32dfce6b4328,

title = "Implications of antimicrobial combinations in complex wound biofilms containing fungi",

abstract = "Diabetic foot ulcer treatment currently focuses on targeting bacterial biofilms, while dismissing fungi. To investigate this we used an in vitro biofilm model containing bacteria and fungi, reflective of the wound environment, to test the impact of antimicrobials. Here we showed that while mono-treatment approaches influenced biofilm composition it had no discernible effect on overall quantity. Only by combining bacterial and fungal specific antibiotics were we able to decrease the biofilm bioburden, irrespective of composition.",

keywords = "Biofilm, Polymicrobial, Chronic wound, Antimicrobial, Candida",

author = "Townsend, \{Eleanor M\} and Leighann Sherry and Ryan Kean and Donald Hansom and Mackay, \{William G\} and Craig Williams and John Butcher and Gordon Ramage",

note = "6 months embargo",

year = "2017",

month = sep,

day = "1",

doi = "10.1128/AAC.00672-17",

language = "English",

volume = "61",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "9",

}

TY - JOUR

T1 - Implications of antimicrobial combinations in complex wound biofilms containing fungi

AU - Townsend, Eleanor M

AU - Sherry, Leighann

AU - Kean, Ryan

AU - Hansom, Donald

AU - Mackay, William G

AU - Williams, Craig

AU - Butcher, John

AU - Ramage, Gordon

N1 - 6 months embargo

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Diabetic foot ulcer treatment currently focuses on targeting bacterial biofilms, while dismissing fungi. To investigate this we used an in vitro biofilm model containing bacteria and fungi, reflective of the wound environment, to test the impact of antimicrobials. Here we showed that while mono-treatment approaches influenced biofilm composition it had no discernible effect on overall quantity. Only by combining bacterial and fungal specific antibiotics were we able to decrease the biofilm bioburden, irrespective of composition.

AB - Diabetic foot ulcer treatment currently focuses on targeting bacterial biofilms, while dismissing fungi. To investigate this we used an in vitro biofilm model containing bacteria and fungi, reflective of the wound environment, to test the impact of antimicrobials. Here we showed that while mono-treatment approaches influenced biofilm composition it had no discernible effect on overall quantity. Only by combining bacterial and fungal specific antibiotics were we able to decrease the biofilm bioburden, irrespective of composition.

KW - Biofilm

KW - Polymicrobial

KW - Chronic wound

KW - Antimicrobial

KW - Candida

UR - https://aac.asm.org/content/61/9/e00672-17/figures-only

U2 - 10.1128/AAC.00672-17

DO - 10.1128/AAC.00672-17

M3 - Article

SN - 0066-4804

VL - 61

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 9

M1 - e00672-17

ER -

Implications of antimicrobial combinations in complex wound biofilms containing fungi

Abstract

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this