Implications of antimicrobial combinations in complex wound biofilms containing fungi

Eleanor M Townsend; Leighann Sherry; Ryan Kean; Donald Hansom; William G Mackay; Craig Williams; John Butcher; Gordon Ramage

doi:10.1128/AAC.00672-17

Implications of antimicrobial combinations in complex wound biofilms containing fungi

Eleanor M Townsend, Leighann Sherry, Ryan Kean, Donald Hansom, William G Mackay, Craig Williams, John Butcher, Gordon Ramage

School of Health and Life Sciences

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

135 Downloads (Pure)

Abstract

Diabetic foot ulcer treatment currently focuses on targeting bacterial biofilms, while dismissing fungi. To investigate this we used an in vitro biofilm model containing bacteria and fungi, reflective of the wound environment, to test the impact of antimicrobials. Here we showed that while mono-treatment approaches influenced biofilm composition it had no discernible effect on overall quantity. Only by combining bacterial and fungal specific antibiotics were we able to decrease the biofilm bioburden, irrespective of composition.

Original language	English
Article number	e00672-17
Number of pages	5
Journal	Antimicrobial Agents and Chemotherapy
Volume	61
Issue number	9
Early online date	10 Jul 2017
DOIs	https://doi.org/10.1128/AAC.00672-17
Publication status	Published - 1 Sept 2017

Keywords

Biofilm
Polymicrobial
Chronic wound
Antimicrobial
Candida

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/AAC.00672-17

2017 07 03 Townsend et al Implications finalAccepted author manuscript, 651 KB

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AU - Townsend, Eleanor M

AU - Sherry, Leighann

AU - Kean, Ryan

AU - Hansom, Donald

AU - Mackay, William G

AU - Williams, Craig

AU - Butcher, John

AU - Ramage, Gordon

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AB - Diabetic foot ulcer treatment currently focuses on targeting bacterial biofilms, while dismissing fungi. To investigate this we used an in vitro biofilm model containing bacteria and fungi, reflective of the wound environment, to test the impact of antimicrobials. Here we showed that while mono-treatment approaches influenced biofilm composition it had no discernible effect on overall quantity. Only by combining bacterial and fungal specific antibiotics were we able to decrease the biofilm bioburden, irrespective of composition.

KW - Biofilm

KW - Polymicrobial

KW - Chronic wound

KW - Antimicrobial

KW - Candida

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IS - 9

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ER -

Implications of antimicrobial combinations in complex wound biofilms containing fungi

Abstract

Keywords

UN SDGs

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