IGFBP-1 as a potential marker of environmental stress in fish

Moira Lewitt, Vasanthi Budige

Research output: Contribution to conferencePaper

Abstract

Insulin-like growth factors have diverse roles in growth and metabolism that are regulated by a family of IGF-binding proteins (IGFBPs). IGFBP-1 is an important inhibitor of IGF action. In mammals hepatic IGFBP-1 in inhibited by insulin, and stimulated by glucocorticoids and other stress-related factors. In zebrafish there are two IGFBP-1 genes and we have shown that expression of each is suppressed in vivo by feeding. We have developed a liver explant culture system to study the direct regulation of Igfbp1a and Igfbp1b in vitro. A 2-h exposure to a maximally effective concentration of dexamethasone stimulated Igfbp1a 44-fold (p<0.001) and Igfbp1b 24-fold (p<0.001), in the presence and absence of inhibition by insulin. A maximally inhibitory concentration of insulin was more effective at short incubation times (15min). Oestrogen stimulated IGFBP-1, particularly at long incubation times (24h). It is speculated that IGFBP-1 expression is a marker of environmental stress in fish.
Original languageEnglish
Publication statusPublished - Aug 2014
Event11th International Congress on the Biology of the Fish - Heriot-Watt University, Edinburgh, United Kingdom
Duration: 3 Aug 20147 Aug 2014

Conference

Conference11th International Congress on the Biology of the Fish
CountryUnited Kingdom
CityEdinburgh
Period3/08/147/08/14

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insulin-like growth factor binding proteins
fish
insulin
liver
somatomedins
dexamethasone
glucocorticoids
Danio rerio
estrogens
explants
mammals
metabolism
genes

Cite this

Lewitt, M., & Budige, V. (2014). IGFBP-1 as a potential marker of environmental stress in fish. Paper presented at 11th International Congress on the Biology of the Fish, Edinburgh, United Kingdom.
Lewitt, Moira ; Budige, Vasanthi. / IGFBP-1 as a potential marker of environmental stress in fish. Paper presented at 11th International Congress on the Biology of the Fish, Edinburgh, United Kingdom.
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Lewitt, M & Budige, V 2014, 'IGFBP-1 as a potential marker of environmental stress in fish' Paper presented at 11th International Congress on the Biology of the Fish, Edinburgh, United Kingdom, 3/08/14 - 7/08/14, .

IGFBP-1 as a potential marker of environmental stress in fish. / Lewitt, Moira; Budige, Vasanthi.

2014. Paper presented at 11th International Congress on the Biology of the Fish, Edinburgh, United Kingdom.

Research output: Contribution to conferencePaper

TY - CONF

T1 - IGFBP-1 as a potential marker of environmental stress in fish

AU - Lewitt, Moira

AU - Budige, Vasanthi

PY - 2014/8

Y1 - 2014/8

N2 - Insulin-like growth factors have diverse roles in growth and metabolism that are regulated by a family of IGF-binding proteins (IGFBPs). IGFBP-1 is an important inhibitor of IGF action. In mammals hepatic IGFBP-1 in inhibited by insulin, and stimulated by glucocorticoids and other stress-related factors. In zebrafish there are two IGFBP-1 genes and we have shown that expression of each is suppressed in vivo by feeding. We have developed a liver explant culture system to study the direct regulation of Igfbp1a and Igfbp1b in vitro. A 2-h exposure to a maximally effective concentration of dexamethasone stimulated Igfbp1a 44-fold (p<0.001) and Igfbp1b 24-fold (p<0.001), in the presence and absence of inhibition by insulin. A maximally inhibitory concentration of insulin was more effective at short incubation times (15min). Oestrogen stimulated IGFBP-1, particularly at long incubation times (24h). It is speculated that IGFBP-1 expression is a marker of environmental stress in fish.

AB - Insulin-like growth factors have diverse roles in growth and metabolism that are regulated by a family of IGF-binding proteins (IGFBPs). IGFBP-1 is an important inhibitor of IGF action. In mammals hepatic IGFBP-1 in inhibited by insulin, and stimulated by glucocorticoids and other stress-related factors. In zebrafish there are two IGFBP-1 genes and we have shown that expression of each is suppressed in vivo by feeding. We have developed a liver explant culture system to study the direct regulation of Igfbp1a and Igfbp1b in vitro. A 2-h exposure to a maximally effective concentration of dexamethasone stimulated Igfbp1a 44-fold (p<0.001) and Igfbp1b 24-fold (p<0.001), in the presence and absence of inhibition by insulin. A maximally inhibitory concentration of insulin was more effective at short incubation times (15min). Oestrogen stimulated IGFBP-1, particularly at long incubation times (24h). It is speculated that IGFBP-1 expression is a marker of environmental stress in fish.

UR - http://www2.hw.ac.uk/sls/icbf2014/Abstract%20book%20-%20online%20version.pdf

M3 - Paper

ER -

Lewitt M, Budige V. IGFBP-1 as a potential marker of environmental stress in fish. 2014. Paper presented at 11th International Congress on the Biology of the Fish, Edinburgh, United Kingdom.