TY - JOUR
T1 - Identified helix neurons
T2 - mutually exclusive expression of the tetrapeptide and heptapeptide members of the FMRFamide family.
AU - Cottrell, G.A.
AU - Price, D.A.
AU - Dobie, K.E.
AU - Hettle, S.
AU - Sommerville, J.
AU - MacDonald, M
PY - 1992
Y1 - 1992
N2 - Extracts were prepared from selected neurons taken from the central ganglia of the pulmonate snail Helix aspersa, and parallel radioimmunoassay (RIA) and high pressure liquid chromatography (HPLC) were used to analyze for FMRFamide-related peptides (FaRPs). Some neurons (e.g., the cerebral C3 neuron) contained only the tetrapeptides related to FMRFamide (tetra-FaRPs), whereas two clusters of neurons in the parietal ganglia contain a preponderance of the hepta-FaRPs. Using cloned cDNA probes in molecular hybridization studies, we showed that the C3 neuron (and some other large neurons) contain the mRNA species that encodes the tetra-FaRPs. In contrast, the neurons of the parietal clusters predominantly express the mRNA that encodes the hepta-FaRPs. These results were confirmed by in situ hybridization with antisense RNA on whole mount ganglia. Each type of experiment supports the view that FaRP-containing Helix neurons express either the tetra-FaRPs or the hepta-FaRPs, but not both.
AB - Extracts were prepared from selected neurons taken from the central ganglia of the pulmonate snail Helix aspersa, and parallel radioimmunoassay (RIA) and high pressure liquid chromatography (HPLC) were used to analyze for FMRFamide-related peptides (FaRPs). Some neurons (e.g., the cerebral C3 neuron) contained only the tetrapeptides related to FMRFamide (tetra-FaRPs), whereas two clusters of neurons in the parietal ganglia contain a preponderance of the hepta-FaRPs. Using cloned cDNA probes in molecular hybridization studies, we showed that the C3 neuron (and some other large neurons) contain the mRNA species that encodes the tetra-FaRPs. In contrast, the neurons of the parietal clusters predominantly express the mRNA that encodes the hepta-FaRPs. These results were confirmed by in situ hybridization with antisense RNA on whole mount ganglia. Each type of experiment supports the view that FaRP-containing Helix neurons express either the tetra-FaRPs or the hepta-FaRPs, but not both.
U2 - 10.2307/1542412
DO - 10.2307/1542412
M3 - Article
SN - 1939-8697
VL - 183
SP - 113
EP - 122
JO - The Biological Bulletin
JF - The Biological Bulletin
IS - 1
ER -