High-dose astaxanthin supplementation suppresses antioxidant enzyme activity during moderate-intensity swimming training in mice

Yingsong Zhou, Julien S. Baker, Xiaoping Chen, Yajun Wang, Haimin Chen, Gareth W. Davison, Xiaojun Yan

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4 Citations (Scopus)
123 Downloads (Pure)

Abstract

Exercise-induced reactive oxygen and nitrogen species are increasingly considered as beneficial health promotion. Astaxanthin (ASX) has been recognized as a potent antioxidant suitable for human ingestion. We investigated whether ASX administration suppressed antioxidant enzyme activity in moderate-intensity exercise. Seven-week-old male C57BL/6 mice ( n = 8/group) were treated with ASX (5, 15, and 30 mg/kg BW) combined with 45 min/day moderate-intensity swimming training for four weeks. Results showed that the mice administrated with 15 and 30 mg/kg of ASX decreased glutathione peroxidase, catalase, malondialdehyde, and creatine kinase levels in plasma or muscle, compared with the swimming control group. Beyond that, these two (15 and 30 mg/kg BW) dosages of ASX downregulated gastrocnemius muscle erythroid 2p45 (NF-E2)-related factor 2 (Nrf2). Meanwhile, mRNA of Nrf2 and Nrf2-dependent enzymes in mice heart were also downregulated in the ASX-treated groups. However, the mice treated with 15 or 30 mg/kg ASX had increased constitutive nitric oxidase synthase and superoxide dismutase activity, compared with the swimming and sedentary control groups. Our findings indicate that high-dose administration of astaxanthin can blunt antioxidant enzyme activity and downregulate transcription of Nrf2 and Nrf2-dependent enzymes along with attenuating plasma and muscle MDA.

Original languageEnglish
Article number1244
Number of pages13
JournalNutrients
Volume11
Issue number6
DOIs
Publication statusPublished - 31 May 2019

Keywords

  • Astaxanthin
  • Antioxidant
  • Oxidative stress
  • Chronic exercise
  • physical adaption

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