Healthy primary epithelial cells show an elevated inflammatory response to bacterial stimulus compared to COPD cells

Stephen A. Carson, James A. Reihill, Catherine R. Fulton, Lorcan P. McGarvey, Fionnuala T. Lundy, Anne Crilly, Keith D. Thornbury

Research output: Contribution to journalMeeting Abstract


Exacerbations are frequently occurring events in the progression of COPD and are characterised by a sudden reduction in lung function associated with increased airway inflammation and sputum production. Triggers such as bacterial infection can lead to hospitalisation and increased mortality.

The aim of this study was to investigate differences in the response of COPD and healthy airway cells to bacterial stimulus by measuring levels of host inflammatory mediators.

Two clinically relevant strains of bacteria (Streptococcus pneumoniae and Haemophilus influenzae) were heat-inactivated and co-cultured at an established non-toxic multiplicity of infection (MOI) 25:1 for 24 hours with primary human airway epithelial cells obtained from a healthy and COPD donor, grown at air-liquid interface. Cell-conditioned media was removed and analysed using the Bio-Plex Pro™ Human Inflammation Panel 1, 37-Plex assay (Bio-Rad)

Cytokines and other pro-inflammatory proteins, as previously established, were generally stimulated in response to heat-inactivated pathogens. However, on assessment of the Bioplex panel as a whole, H. influenzae showed increased potential to induce the production of cytokines/proteins to a greater level than S. pneumoniae. The greatest overall increases were seen with the healthy cells (28/37 analytes), indicating that they are potentially more capable of mounting an acute inflammatory response than COPD cells.
Original languageEnglish
Article number127.7
JournalThe FASEB Journal
Issue numberSupplement 1
Publication statusE-pub ahead of print - 1 Apr 2019
EventExperimental Biology 2019 - Orange County Convention Center, Orlando, United States
Duration: 6 Apr 20199 Apr 2019


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