Abstract
The traditional requirement for serum for the growth of cell lines in culture can often be provided by serum-free media containing a variety of growth factors. An alternative approach, which is investigated here, is to engineer the cells to express growth factors in an endogenous fashion. We report here on the growth of NM3T3 cells transfected with basic fibroblast growth factor (bFGF) andor insulin-like growth factors I and II (IGF-I and IGF-II). Morphological changes and the growth rate were compared with growth in normal serum-containing medium. The experimental data suggested that the expression
of either bFGF alone, or the co-expression of bFGF and either IGF-I or 11 could
improve the survival of NIH3T3 cells in low serum or serum-free media. The use of such lines could decrease the use of serum in cell culture and thus both reduce the costs involved in this technique and simplify the down-stream purification procedure in protein harvest. Hence, such lines may be of value in both experimental and industrial applications
of either bFGF alone, or the co-expression of bFGF and either IGF-I or 11 could
improve the survival of NIH3T3 cells in low serum or serum-free media. The use of such lines could decrease the use of serum in cell culture and thus both reduce the costs involved in this technique and simplify the down-stream purification procedure in protein harvest. Hence, such lines may be of value in both experimental and industrial applications
| Original language | English |
|---|---|
| Title of host publication | Animal Cell Technology |
| Subtitle of host publication | challenges for the 21st Century |
| Publisher | Springer |
| Pages | 283-287 |
| Number of pages | 5 |
| ISBN (Electronic) | 9780306468698 |
| ISBN (Print) | 9780792358053 |
| Publication status | Published - 1999 |
| Externally published | Yes |
Keywords
- Basic Fibroblast Growth Factor
- NIH3T3 Cell
- Transfected Cell Line
- Recombinant Growth Factor
- Rabbit Articular Chondrocytes