Growth characteristics of NIH3T3 cells expressing bFGF and/or IGF-I and/or IGF-II. 

Dajiang Li, Simon Hettle, John McLean, Caroline MacDonald

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The traditional requirement for serum for the growth of cell lines in culture can often be provided by serum-free media containing a variety of growth factors. An alternative approach, which is investigated here, is to engineer the cells to express growth factors in an endogenous fashion. We report here on the growth of NM3T3 cells transfected with basic fibroblast growth factor (bFGF) andor insulin-like growth factors I and II (IGF-I and IGF-II). Morphological changes and the growth rate were compared with growth in normal serum-containing medium. The experimental data suggested that the expression
of either bFGF alone, or the co-expression of bFGF and either IGF-I or 11 could
improve the survival of NIH3T3 cells in low serum or serum-free media. The use of such lines could decrease the use of serum in cell culture and thus both reduce the costs involved in this technique and simplify the down-stream purification procedure in protein harvest. Hence, such lines may be of value in both experimental and industrial applications
Original languageEnglish
Title of host publicationAnimal Cell Technology
Subtitle of host publicationchallenges for the 21st Century
PublisherSpringer
Pages283-287
Number of pages5
ISBN (Electronic)9780306468698
ISBN (Print)9780792358053
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

insulin-like growth factor II
fibroblast growth factor 2
insulin-like growth factor I
growth factors
industrial applications
cells
engineers
cell viability
cell growth
cell culture
cell lines
proteins
methodology

Keywords

  • Basic Fibroblast Growth Factor
  • NIH3T3 Cell
  • Transfected Cell Line
  • Recombinant Growth Factor
  • Rabbit Articular Chondrocytes

Cite this

Li, D., Hettle, S., McLean, J., & MacDonald, C. (1999). Growth characteristics of NIH3T3 cells expressing bFGF and/or IGF-I and/or IGF-II. . In Animal Cell Technology: challenges for the 21st Century (pp. 283-287). Springer.
Li, Dajiang ; Hettle, Simon ; McLean, John ; MacDonald, Caroline. / Growth characteristics of NIH3T3 cells expressing bFGF and/or IGF-I and/or IGF-II. . Animal Cell Technology: challenges for the 21st Century. Springer, 1999. pp. 283-287
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abstract = "The traditional requirement for serum for the growth of cell lines in culture can often be provided by serum-free media containing a variety of growth factors. An alternative approach, which is investigated here, is to engineer the cells to express growth factors in an endogenous fashion. We report here on the growth of NM3T3 cells transfected with basic fibroblast growth factor (bFGF) andor insulin-like growth factors I and II (IGF-I and IGF-II). Morphological changes and the growth rate were compared with growth in normal serum-containing medium. The experimental data suggested that the expressionof either bFGF alone, or the co-expression of bFGF and either IGF-I or 11 couldimprove the survival of NIH3T3 cells in low serum or serum-free media. The use of such lines could decrease the use of serum in cell culture and thus both reduce the costs involved in this technique and simplify the down-stream purification procedure in protein harvest. Hence, such lines may be of value in both experimental and industrial applications",
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Li, D, Hettle, S, McLean, J & MacDonald, C 1999, Growth characteristics of NIH3T3 cells expressing bFGF and/or IGF-I and/or IGF-II. . in Animal Cell Technology: challenges for the 21st Century. Springer, pp. 283-287.

Growth characteristics of NIH3T3 cells expressing bFGF and/or IGF-I and/or IGF-II. . / Li, Dajiang; Hettle, Simon; McLean, John; MacDonald, Caroline.

Animal Cell Technology: challenges for the 21st Century. Springer, 1999. p. 283-287.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - Growth characteristics of NIH3T3 cells expressing bFGF and/or IGF-I and/or IGF-II. 

AU - Li, Dajiang

AU - Hettle, Simon

AU - McLean, John

AU - MacDonald, Caroline

N1 - No full details of meeting event available. Only location of Kyoto, Japan and year 1998

PY - 1999

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N2 - The traditional requirement for serum for the growth of cell lines in culture can often be provided by serum-free media containing a variety of growth factors. An alternative approach, which is investigated here, is to engineer the cells to express growth factors in an endogenous fashion. We report here on the growth of NM3T3 cells transfected with basic fibroblast growth factor (bFGF) andor insulin-like growth factors I and II (IGF-I and IGF-II). Morphological changes and the growth rate were compared with growth in normal serum-containing medium. The experimental data suggested that the expressionof either bFGF alone, or the co-expression of bFGF and either IGF-I or 11 couldimprove the survival of NIH3T3 cells in low serum or serum-free media. The use of such lines could decrease the use of serum in cell culture and thus both reduce the costs involved in this technique and simplify the down-stream purification procedure in protein harvest. Hence, such lines may be of value in both experimental and industrial applications

AB - The traditional requirement for serum for the growth of cell lines in culture can often be provided by serum-free media containing a variety of growth factors. An alternative approach, which is investigated here, is to engineer the cells to express growth factors in an endogenous fashion. We report here on the growth of NM3T3 cells transfected with basic fibroblast growth factor (bFGF) andor insulin-like growth factors I and II (IGF-I and IGF-II). Morphological changes and the growth rate were compared with growth in normal serum-containing medium. The experimental data suggested that the expressionof either bFGF alone, or the co-expression of bFGF and either IGF-I or 11 couldimprove the survival of NIH3T3 cells in low serum or serum-free media. The use of such lines could decrease the use of serum in cell culture and thus both reduce the costs involved in this technique and simplify the down-stream purification procedure in protein harvest. Hence, such lines may be of value in both experimental and industrial applications

KW - Basic Fibroblast Growth Factor

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KW - Transfected Cell Line

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Li D, Hettle S, McLean J, MacDonald C. Growth characteristics of NIH3T3 cells expressing bFGF and/or IGF-I and/or IGF-II. . In Animal Cell Technology: challenges for the 21st Century. Springer. 1999. p. 283-287