Functionalised Nanoparticles for Nucleic Acid Sequence Analysis Using Optical Spectroscopies

Duncan Graham, Karen Faulds, David Thompson, Fiona MacKenzie, Robert Stokes, Alexandra MacAskill

Research output: Contribution to journalArticle

Abstract

SERRS (surface-enhanced resonance Raman scattering) is a vibrational spectroscopy which allows extremely sensitive and selective detection of labelled DNA sequences with detection limits which rival, and in most cases surpass, that of fluorescence. SERRS relies on a visible chromophore adsorbing on to an enhancing surface. DNA itself is not SERRS-active, as it lacks a suitable visible chromophore and has poor adsorption properties on to the surfaces used for enhancement. The surface normally used for enhancement in these sorts of studies are metallic nanoparticles and, through modification of DNA probes by the addition of suitable SERRS labels, signals can be obtained that are highly sensitive and very selective. The aggregation state of the nanoparticles is critical to the sensitivity, and, in the present paper, we show how straightforward detection of labelled DNA probes can be achieved using SERRS in a quantitative manner and with a variety of different commercially available labels. In a second approach, we show how the properties of aggregation to turn on the SERRS effect can be exploited through DNA hybridization to give identification of a particular DNA sequence. This approach lends itself to closed-tube formats and is a promising way forward for molecular diagnostics using SERRS.
Original languageEnglish
Pages (from-to)441-444
JournalBiochemical Society Transactions
Volume37
Issue number2
DOIs
Publication statusPublished - 1 Apr 2009

Fingerprint

Nucleic acid sequences
Raman Spectrum Analysis
Nanoparticles
Nucleic Acids
Sequence Analysis
Spectrum Analysis
Raman scattering
DNA Probes
DNA sequences
Chromophores
Metal Nanoparticles
Labels
Molecular Pathology
DNA
Agglomeration
Optical spectroscopy
Adsorption
Vibrational spectroscopy
Limit of Detection
Fluorescence

Cite this

Graham, Duncan ; Faulds, Karen ; Thompson, David ; MacKenzie, Fiona ; Stokes, Robert ; MacAskill, Alexandra. / Functionalised Nanoparticles for Nucleic Acid Sequence Analysis Using Optical Spectroscopies. In: Biochemical Society Transactions. 2009 ; Vol. 37, No. 2. pp. 441-444.
@article{202b543e423f48d188c9cc129a560306,
title = "Functionalised Nanoparticles for Nucleic Acid Sequence Analysis Using Optical Spectroscopies",
abstract = "SERRS (surface-enhanced resonance Raman scattering) is a vibrational spectroscopy which allows extremely sensitive and selective detection of labelled DNA sequences with detection limits which rival, and in most cases surpass, that of fluorescence. SERRS relies on a visible chromophore adsorbing on to an enhancing surface. DNA itself is not SERRS-active, as it lacks a suitable visible chromophore and has poor adsorption properties on to the surfaces used for enhancement. The surface normally used for enhancement in these sorts of studies are metallic nanoparticles and, through modification of DNA probes by the addition of suitable SERRS labels, signals can be obtained that are highly sensitive and very selective. The aggregation state of the nanoparticles is critical to the sensitivity, and, in the present paper, we show how straightforward detection of labelled DNA probes can be achieved using SERRS in a quantitative manner and with a variety of different commercially available labels. In a second approach, we show how the properties of aggregation to turn on the SERRS effect can be exploited through DNA hybridization to give identification of a particular DNA sequence. This approach lends itself to closed-tube formats and is a promising way forward for molecular diagnostics using SERRS.",
author = "Duncan Graham and Karen Faulds and David Thompson and Fiona MacKenzie and Robert Stokes and Alexandra MacAskill",
year = "2009",
month = "4",
day = "1",
doi = "10.1042/BST0370441",
language = "English",
volume = "37",
pages = "441--444",
journal = "Biochemical Society Transactions",
issn = "0300-5127",
publisher = "Portland Press Limited",
number = "2",

}

Functionalised Nanoparticles for Nucleic Acid Sequence Analysis Using Optical Spectroscopies. / Graham, Duncan; Faulds, Karen; Thompson, David; MacKenzie, Fiona; Stokes, Robert; MacAskill, Alexandra.

In: Biochemical Society Transactions, Vol. 37, No. 2, 01.04.2009, p. 441-444.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Functionalised Nanoparticles for Nucleic Acid Sequence Analysis Using Optical Spectroscopies

AU - Graham, Duncan

AU - Faulds, Karen

AU - Thompson, David

AU - MacKenzie, Fiona

AU - Stokes, Robert

AU - MacAskill, Alexandra

PY - 2009/4/1

Y1 - 2009/4/1

N2 - SERRS (surface-enhanced resonance Raman scattering) is a vibrational spectroscopy which allows extremely sensitive and selective detection of labelled DNA sequences with detection limits which rival, and in most cases surpass, that of fluorescence. SERRS relies on a visible chromophore adsorbing on to an enhancing surface. DNA itself is not SERRS-active, as it lacks a suitable visible chromophore and has poor adsorption properties on to the surfaces used for enhancement. The surface normally used for enhancement in these sorts of studies are metallic nanoparticles and, through modification of DNA probes by the addition of suitable SERRS labels, signals can be obtained that are highly sensitive and very selective. The aggregation state of the nanoparticles is critical to the sensitivity, and, in the present paper, we show how straightforward detection of labelled DNA probes can be achieved using SERRS in a quantitative manner and with a variety of different commercially available labels. In a second approach, we show how the properties of aggregation to turn on the SERRS effect can be exploited through DNA hybridization to give identification of a particular DNA sequence. This approach lends itself to closed-tube formats and is a promising way forward for molecular diagnostics using SERRS.

AB - SERRS (surface-enhanced resonance Raman scattering) is a vibrational spectroscopy which allows extremely sensitive and selective detection of labelled DNA sequences with detection limits which rival, and in most cases surpass, that of fluorescence. SERRS relies on a visible chromophore adsorbing on to an enhancing surface. DNA itself is not SERRS-active, as it lacks a suitable visible chromophore and has poor adsorption properties on to the surfaces used for enhancement. The surface normally used for enhancement in these sorts of studies are metallic nanoparticles and, through modification of DNA probes by the addition of suitable SERRS labels, signals can be obtained that are highly sensitive and very selective. The aggregation state of the nanoparticles is critical to the sensitivity, and, in the present paper, we show how straightforward detection of labelled DNA probes can be achieved using SERRS in a quantitative manner and with a variety of different commercially available labels. In a second approach, we show how the properties of aggregation to turn on the SERRS effect can be exploited through DNA hybridization to give identification of a particular DNA sequence. This approach lends itself to closed-tube formats and is a promising way forward for molecular diagnostics using SERRS.

U2 - 10.1042/BST0370441

DO - 10.1042/BST0370441

M3 - Article

VL - 37

SP - 441

EP - 444

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

SN - 0300-5127

IS - 2

ER -