Expression and modulation of TUB by insulin and thyroid hormone in primary rat and murine 3T3-L1 adipocytes

Clare Stretton, Gary J. Litherland, Audrey Moynihan, Eric Hajduch, Harinder S. Hundal

Research output: Contribution to journalArticle

Abstract

tub encodes a protein of poorly understood function, but one implicated strongly in the control of energy balance and insulin sensitivity. Whilst tub expression is particularly prominent in neurones it is also detectable in extraneuronal tissues. We show here, for the first time, expression of TUB protein in rat adipocytes and the murine adipocyte model 3T3-L1 and demonstrate that insulin induces its tyrosine phosphorylation and association with the insulin receptor. TUB expression is regulated developmentally during adipogenic differentiation of 3T3-L1 cells and in response to cell treatment with thyroid hormone or induction of insulin resistance. TUB was upregulated 5- to 10-fold in adipocytes from obese Zucker rats and 3T3-L1 adipocytes that had been rendered insulin resistant, a response that could be antagonised by rosiglitasone, an insulin-sensitising drug. Our data are consistent with a previously unforeseen role for TUB in insulin signalling and fuel homeostasis in adipocytes.
Original languageEnglish
Pages (from-to)1328-1333
JournalBiochemical and Biophysical Research Communications
Volume390
Issue number4
DOIs
Publication statusPublished - 25 Dec 2009
Externally publishedYes

Keywords

  • 3T3-L1
  • Adipogenesis
  • Zucker rat
  • Insulin
  • Tubby

Cite this

Stretton, Clare ; Litherland, Gary J. ; Moynihan, Audrey ; Hajduch, Eric ; Hundal, Harinder S. / Expression and modulation of TUB by insulin and thyroid hormone in primary rat and murine 3T3-L1 adipocytes. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 390, No. 4. pp. 1328-1333.
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Expression and modulation of TUB by insulin and thyroid hormone in primary rat and murine 3T3-L1 adipocytes. / Stretton, Clare; Litherland, Gary J.; Moynihan, Audrey; Hajduch, Eric; Hundal, Harinder S.

In: Biochemical and Biophysical Research Communications, Vol. 390, No. 4, 25.12.2009, p. 1328-1333.

Research output: Contribution to journalArticle

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T1 - Expression and modulation of TUB by insulin and thyroid hormone in primary rat and murine 3T3-L1 adipocytes

AU - Stretton, Clare

AU - Litherland, Gary J.

AU - Moynihan, Audrey

AU - Hajduch, Eric

AU - Hundal, Harinder S.

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AB - tub encodes a protein of poorly understood function, but one implicated strongly in the control of energy balance and insulin sensitivity. Whilst tub expression is particularly prominent in neurones it is also detectable in extraneuronal tissues. We show here, for the first time, expression of TUB protein in rat adipocytes and the murine adipocyte model 3T3-L1 and demonstrate that insulin induces its tyrosine phosphorylation and association with the insulin receptor. TUB expression is regulated developmentally during adipogenic differentiation of 3T3-L1 cells and in response to cell treatment with thyroid hormone or induction of insulin resistance. TUB was upregulated 5- to 10-fold in adipocytes from obese Zucker rats and 3T3-L1 adipocytes that had been rendered insulin resistant, a response that could be antagonised by rosiglitasone, an insulin-sensitising drug. Our data are consistent with a previously unforeseen role for TUB in insulin signalling and fuel homeostasis in adipocytes.

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KW - Adipogenesis

KW - Zucker rat

KW - Insulin

KW - Tubby

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