Experimental arthritis exacerbates periodontal disease but periodontal infection does not exacerbated experimental arthritis

John Butcher, Jessica Oliver-Bell, Robert Benson, James Brewer, Paul Garside, Iain B. McInnes, Shauna Culshaw

Research output: Contribution to journalMeeting Abstract

Abstract

Background/Purpose
There is a proposed link between periodontal disease
(PD) and rheumatoid arthritis (RA). A substantial, although not unanimous, body of epidemiological evidence indicates a bidirectional link, with patients with PD more likely to suffer RA and vice versa. Several mechanisms have been proposed to explain the relationship between PD and RA, including i) periodontal bacterial generating autoantigens, ii) local periodontal inflammation generating autoantigens, ii) direct bacterial insult to the joint, iv) systemic inflammation associated with periodontitis exacerbating inflammation at distant sites, and v) shared risk factors. Previous studies, using experimental models of later stages of arthritis and joint destruction, suggest that periodontal infection may exacerbate arthritis and vice versa. We sought to investigate whether periodontal infection may impact on the initiation of arthritis and therefore used an experimental model of breach of immune tolerance, generation of autoantibodies and synovitis.

Methods
Mice were orally infected with P. gingivalis to initiate alveolar bone destruction, modeling human periodontal disease. Animals subsequently received Th1 polarized ovalbumin specific T cells by adoptive transfer, and
were immunized, and then challenged in the footpad with ovalbumin. Paw swelling, joint histology, periodontal bone loss, responses of T cells from the
draining lymph nodes, and serum antibody were assessed.

Results
Animals developed footpad swelling and histological evidence of synovitis. Circulating anti-CCP antibodies were significantly elevated in experimental arthritis compared with control animals (arthritis: 1189 25.56 ELISA units (EU), control: 934.1 99.39 EU; p0.05), and a similar trend was observed for circulating anti-collagen antibodies, although this did not reach
statistical significance (arthritis: 439.5 124.2 EU, control: 224.0 68.8 EU; p0.19). Periodontal infection had no impact on any measures of this model of
early arthritis. However, animals with periodontal infection and arthritis showed significantly more periodontal bone destruction (periodontal infection+arthritis: 0.44 0.01 mm, periodontal infection: 0.40 0.01 mm; p0.05) compared with
animals with periodontal infection alone.

Conclusion
These data suggest that periodontal infection may not be responsible for initiating the breach of tolerance associated with the initiation of arthritis. In this model system of breach of tolerance, oral infection made no
impact on articular or autoantibody outcomes. Nonetheless, established periodontal inflammation may be detrimental to patients with RA and therefore further
human and animal studies are required to unravel the mechanisms mediating the PD/RA relationship as understanding such a relationship offers potentially
improved therapeutic strategies – for both PD and RA independently and in combination.
Original languageEnglish
Pages (from-to)S944-S945
Number of pages2
JournalArthritis and Rheumatism
Volume65
Issue numberS10
DOIs
Publication statusPublished - 2013
Externally publishedYes
EventAmerican College of Rheumatology 2013 Annual Meeting - San Diego, United States
Duration: 25 Oct 201330 Oct 2013

Fingerprint

Experimental Arthritis
Periodontal Diseases
Arthritis
Rheumatoid Arthritis
Infection
Joints
Enzyme-Linked Immunosorbent Assay
Inflammation
Synovitis
Ovalbumin
Autoantigens
Autoantibodies
Anti-Idiotypic Antibodies
Theoretical Models
Alveolar Bone Loss
T-Lymphocytes
Bone and Bones
Immune Tolerance
Adoptive Transfer
Periodontitis

Cite this

Butcher, J., Oliver-Bell, J., Benson, R., Brewer, J., Garside, P., McInnes, I. B., & Culshaw, S. (2013). Experimental arthritis exacerbates periodontal disease but periodontal infection does not exacerbated experimental arthritis. Arthritis and Rheumatism, 65(S10), S944-S945. https://doi.org/10.1002/art.38216
Butcher, John ; Oliver-Bell, Jessica ; Benson, Robert ; Brewer, James ; Garside, Paul ; McInnes, Iain B. ; Culshaw, Shauna. / Experimental arthritis exacerbates periodontal disease but periodontal infection does not exacerbated experimental arthritis. In: Arthritis and Rheumatism. 2013 ; Vol. 65, No. S10. pp. S944-S945.
@article{205bc29e428f4683b1dc3b1b709cb312,
title = "Experimental arthritis exacerbates periodontal disease but periodontal infection does not exacerbated experimental arthritis",
abstract = "Background/PurposeThere is a proposed link between periodontal disease(PD) and rheumatoid arthritis (RA). A substantial, although not unanimous, body of epidemiological evidence indicates a bidirectional link, with patients with PD more likely to suffer RA and vice versa. Several mechanisms have been proposed to explain the relationship between PD and RA, including i) periodontal bacterial generating autoantigens, ii) local periodontal inflammation generating autoantigens, ii) direct bacterial insult to the joint, iv) systemic inflammation associated with periodontitis exacerbating inflammation at distant sites, and v) shared risk factors. Previous studies, using experimental models of later stages of arthritis and joint destruction, suggest that periodontal infection may exacerbate arthritis and vice versa. We sought to investigate whether periodontal infection may impact on the initiation of arthritis and therefore used an experimental model of breach of immune tolerance, generation of autoantibodies and synovitis.MethodsMice were orally infected with P. gingivalis to initiate alveolar bone destruction, modeling human periodontal disease. Animals subsequently received Th1 polarized ovalbumin specific T cells by adoptive transfer, andwere immunized, and then challenged in the footpad with ovalbumin. Paw swelling, joint histology, periodontal bone loss, responses of T cells from thedraining lymph nodes, and serum antibody were assessed.ResultsAnimals developed footpad swelling and histological evidence of synovitis. Circulating anti-CCP antibodies were significantly elevated in experimental arthritis compared with control animals (arthritis: 1189 25.56 ELISA units (EU), control: 934.1 99.39 EU; p0.05), and a similar trend was observed for circulating anti-collagen antibodies, although this did not reachstatistical significance (arthritis: 439.5 124.2 EU, control: 224.0 68.8 EU; p0.19). Periodontal infection had no impact on any measures of this model ofearly arthritis. However, animals with periodontal infection and arthritis showed significantly more periodontal bone destruction (periodontal infection+arthritis: 0.44 0.01 mm, periodontal infection: 0.40 0.01 mm; p0.05) compared withanimals with periodontal infection alone.ConclusionThese data suggest that periodontal infection may not be responsible for initiating the breach of tolerance associated with the initiation of arthritis. In this model system of breach of tolerance, oral infection made noimpact on articular or autoantibody outcomes. Nonetheless, established periodontal inflammation may be detrimental to patients with RA and therefore furtherhuman and animal studies are required to unravel the mechanisms mediating the PD/RA relationship as understanding such a relationship offers potentiallyimproved therapeutic strategies – for both PD and RA independently and in combination.",
author = "John Butcher and Jessica Oliver-Bell and Robert Benson and James Brewer and Paul Garside and McInnes, {Iain B.} and Shauna Culshaw",
year = "2013",
doi = "10.1002/art.38216",
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Butcher, J, Oliver-Bell, J, Benson, R, Brewer, J, Garside, P, McInnes, IB & Culshaw, S 2013, 'Experimental arthritis exacerbates periodontal disease but periodontal infection does not exacerbated experimental arthritis' Arthritis and Rheumatism, vol. 65, no. S10, pp. S944-S945. https://doi.org/10.1002/art.38216

Experimental arthritis exacerbates periodontal disease but periodontal infection does not exacerbated experimental arthritis. / Butcher, John; Oliver-Bell, Jessica; Benson, Robert; Brewer, James; Garside, Paul; McInnes, Iain B. ; Culshaw, Shauna.

In: Arthritis and Rheumatism, Vol. 65, No. S10, 2013, p. S944-S945.

Research output: Contribution to journalMeeting Abstract

TY - JOUR

T1 - Experimental arthritis exacerbates periodontal disease but periodontal infection does not exacerbated experimental arthritis

AU - Butcher, John

AU - Oliver-Bell, Jessica

AU - Benson, Robert

AU - Brewer, James

AU - Garside, Paul

AU - McInnes, Iain B.

AU - Culshaw, Shauna

PY - 2013

Y1 - 2013

N2 - Background/PurposeThere is a proposed link between periodontal disease(PD) and rheumatoid arthritis (RA). A substantial, although not unanimous, body of epidemiological evidence indicates a bidirectional link, with patients with PD more likely to suffer RA and vice versa. Several mechanisms have been proposed to explain the relationship between PD and RA, including i) periodontal bacterial generating autoantigens, ii) local periodontal inflammation generating autoantigens, ii) direct bacterial insult to the joint, iv) systemic inflammation associated with periodontitis exacerbating inflammation at distant sites, and v) shared risk factors. Previous studies, using experimental models of later stages of arthritis and joint destruction, suggest that periodontal infection may exacerbate arthritis and vice versa. We sought to investigate whether periodontal infection may impact on the initiation of arthritis and therefore used an experimental model of breach of immune tolerance, generation of autoantibodies and synovitis.MethodsMice were orally infected with P. gingivalis to initiate alveolar bone destruction, modeling human periodontal disease. Animals subsequently received Th1 polarized ovalbumin specific T cells by adoptive transfer, andwere immunized, and then challenged in the footpad with ovalbumin. Paw swelling, joint histology, periodontal bone loss, responses of T cells from thedraining lymph nodes, and serum antibody were assessed.ResultsAnimals developed footpad swelling and histological evidence of synovitis. Circulating anti-CCP antibodies were significantly elevated in experimental arthritis compared with control animals (arthritis: 1189 25.56 ELISA units (EU), control: 934.1 99.39 EU; p0.05), and a similar trend was observed for circulating anti-collagen antibodies, although this did not reachstatistical significance (arthritis: 439.5 124.2 EU, control: 224.0 68.8 EU; p0.19). Periodontal infection had no impact on any measures of this model ofearly arthritis. However, animals with periodontal infection and arthritis showed significantly more periodontal bone destruction (periodontal infection+arthritis: 0.44 0.01 mm, periodontal infection: 0.40 0.01 mm; p0.05) compared withanimals with periodontal infection alone.ConclusionThese data suggest that periodontal infection may not be responsible for initiating the breach of tolerance associated with the initiation of arthritis. In this model system of breach of tolerance, oral infection made noimpact on articular or autoantibody outcomes. Nonetheless, established periodontal inflammation may be detrimental to patients with RA and therefore furtherhuman and animal studies are required to unravel the mechanisms mediating the PD/RA relationship as understanding such a relationship offers potentiallyimproved therapeutic strategies – for both PD and RA independently and in combination.

AB - Background/PurposeThere is a proposed link between periodontal disease(PD) and rheumatoid arthritis (RA). A substantial, although not unanimous, body of epidemiological evidence indicates a bidirectional link, with patients with PD more likely to suffer RA and vice versa. Several mechanisms have been proposed to explain the relationship between PD and RA, including i) periodontal bacterial generating autoantigens, ii) local periodontal inflammation generating autoantigens, ii) direct bacterial insult to the joint, iv) systemic inflammation associated with periodontitis exacerbating inflammation at distant sites, and v) shared risk factors. Previous studies, using experimental models of later stages of arthritis and joint destruction, suggest that periodontal infection may exacerbate arthritis and vice versa. We sought to investigate whether periodontal infection may impact on the initiation of arthritis and therefore used an experimental model of breach of immune tolerance, generation of autoantibodies and synovitis.MethodsMice were orally infected with P. gingivalis to initiate alveolar bone destruction, modeling human periodontal disease. Animals subsequently received Th1 polarized ovalbumin specific T cells by adoptive transfer, andwere immunized, and then challenged in the footpad with ovalbumin. Paw swelling, joint histology, periodontal bone loss, responses of T cells from thedraining lymph nodes, and serum antibody were assessed.ResultsAnimals developed footpad swelling and histological evidence of synovitis. Circulating anti-CCP antibodies were significantly elevated in experimental arthritis compared with control animals (arthritis: 1189 25.56 ELISA units (EU), control: 934.1 99.39 EU; p0.05), and a similar trend was observed for circulating anti-collagen antibodies, although this did not reachstatistical significance (arthritis: 439.5 124.2 EU, control: 224.0 68.8 EU; p0.19). Periodontal infection had no impact on any measures of this model ofearly arthritis. However, animals with periodontal infection and arthritis showed significantly more periodontal bone destruction (periodontal infection+arthritis: 0.44 0.01 mm, periodontal infection: 0.40 0.01 mm; p0.05) compared withanimals with periodontal infection alone.ConclusionThese data suggest that periodontal infection may not be responsible for initiating the breach of tolerance associated with the initiation of arthritis. In this model system of breach of tolerance, oral infection made noimpact on articular or autoantibody outcomes. Nonetheless, established periodontal inflammation may be detrimental to patients with RA and therefore furtherhuman and animal studies are required to unravel the mechanisms mediating the PD/RA relationship as understanding such a relationship offers potentiallyimproved therapeutic strategies – for both PD and RA independently and in combination.

U2 - 10.1002/art.38216

DO - 10.1002/art.38216

M3 - Meeting Abstract

VL - 65

SP - S944-S945

JO - Arthritis and Rheumatism

JF - Arthritis and Rheumatism

SN - 0004-3591

IS - S10

ER -