Evaluation of the lethal & sub-lethal toxicity and potential endocrine disrupting effect of nonylphenol on the  zebra mussel (Dreissena polymorpha).

Brian Quinn, Francois Gagne, Christian Blaise, Mark J. Costello, James G. Wilson, Carmel Mothersill

Research output: Contribution to journalArticle

3 Downloads (Pure)

Abstract

Nonylphenol (NP) is commonly found in surface waters nearby municipal wastewater treatment plants and was shown to have endocrine disrupting effects in aquatic organisms. The purpose of this study was to investigate the toxicity and potential endocrine disrupting effects of NP on the freshwater zebra mussel (Dreissena polymorpha). Toxicity assessment yielded LC50 values of 3.68, 2.19 and 1.62 mg L− 1 after 15, 35 and 50 days of exposure, respectively. LC10 values of 1.6, 1.11 and 0.68 mg L− 1 were respectively obtained for similar exposure periods. At concentrations > 5 mg L− 1, mortality effects were significant, as were those relating to attachment and siphon extension (indicating filtration), both general indicators of health. Endocrine disruption effects were investigated after a prolonged exposure (112 d) to 5 and 500 μg L− 1 NP by measuring Vitellin (Vn)-like protein levels using the alkali-labile phosphate (ALP) assay and gel electrophoresis (GE). An increase in ALP levels was observed in both male and female mussels, although only marginal owing to a significant decrease in the mussels' health indicated by its condition, during the experiment. These levels, however, increased proportionally with NP concentration. Using solid phase thin-layer chromatography, we confirmed increased levels of the steroid cholesterol and evidence of NP uptake. Cholesterol levels in gonad tissue proved to be a more responsive biomarker of exposure to NP than levels of ALP. Further implications relating to the occurrence of endocrine disruption in the zebra mussel are discussed.
Original languageEnglish
Pages (from-to)118-127
Number of pages10
JournalComparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Volume142
Issue number1-2
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Dreissena
Toxicity
Alkalies
Bivalvia
Phosphates
Vitellins
Cholesterol
Health
Aquatic organisms
Siphons
Thin layer chromatography
Aquatic Organisms
Gonads
Biomarkers
Thin Layer Chromatography
Waste Water
Electrophoresis
Fresh Water
Surface waters
Wastewater treatment

Keywords

  • Nonylphenol
  • Zebra mussel
  • Toxicity
  • Endocrine disruption

Cite this

@article{995d3e8d6dcc4d2c9dd97bfba99d6552,
title = "Evaluation of the lethal & sub-lethal toxicity and potential endocrine disrupting effect of nonylphenol on the  zebra mussel (Dreissena polymorpha).",
abstract = "Nonylphenol (NP) is commonly found in surface waters nearby municipal wastewater treatment plants and was shown to have endocrine disrupting effects in aquatic organisms. The purpose of this study was to investigate the toxicity and potential endocrine disrupting effects of NP on the freshwater zebra mussel (Dreissena polymorpha). Toxicity assessment yielded LC50 values of 3.68, 2.19 and 1.62 mg L− 1 after 15, 35 and 50 days of exposure, respectively. LC10 values of 1.6, 1.11 and 0.68 mg L− 1 were respectively obtained for similar exposure periods. At concentrations > 5 mg L− 1, mortality effects were significant, as were those relating to attachment and siphon extension (indicating filtration), both general indicators of health. Endocrine disruption effects were investigated after a prolonged exposure (112 d) to 5 and 500 μg L− 1 NP by measuring Vitellin (Vn)-like protein levels using the alkali-labile phosphate (ALP) assay and gel electrophoresis (GE). An increase in ALP levels was observed in both male and female mussels, although only marginal owing to a significant decrease in the mussels' health indicated by its condition, during the experiment. These levels, however, increased proportionally with NP concentration. Using solid phase thin-layer chromatography, we confirmed increased levels of the steroid cholesterol and evidence of NP uptake. Cholesterol levels in gonad tissue proved to be a more responsive biomarker of exposure to NP than levels of ALP. Further implications relating to the occurrence of endocrine disruption in the zebra mussel are discussed.",
keywords = "Nonylphenol, Zebra mussel, Toxicity, Endocrine disruption",
author = "Brian Quinn and Francois Gagne and Christian Blaise and Costello, {Mark J.} and Wilson, {James G.} and Carmel Mothersill",
year = "2006",
doi = "10.1016/j.cbpc.2005.11.004",
language = "English",
volume = "142",
pages = "118--127",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
issn = "1532-0456",
publisher = "Elsevier B.V.",
number = "1-2",

}

Evaluation of the lethal & sub-lethal toxicity and potential endocrine disrupting effect of nonylphenol on the  zebra mussel (Dreissena polymorpha). / Quinn, Brian; Gagne, Francois; Blaise, Christian; Costello, Mark J.; Wilson, James G.; Mothersill, Carmel.

In: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, Vol. 142, No. 1-2, 2006, p. 118-127.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evaluation of the lethal & sub-lethal toxicity and potential endocrine disrupting effect of nonylphenol on the  zebra mussel (Dreissena polymorpha).

AU - Quinn, Brian

AU - Gagne, Francois

AU - Blaise, Christian

AU - Costello, Mark J.

AU - Wilson, James G.

AU - Mothersill, Carmel

PY - 2006

Y1 - 2006

N2 - Nonylphenol (NP) is commonly found in surface waters nearby municipal wastewater treatment plants and was shown to have endocrine disrupting effects in aquatic organisms. The purpose of this study was to investigate the toxicity and potential endocrine disrupting effects of NP on the freshwater zebra mussel (Dreissena polymorpha). Toxicity assessment yielded LC50 values of 3.68, 2.19 and 1.62 mg L− 1 after 15, 35 and 50 days of exposure, respectively. LC10 values of 1.6, 1.11 and 0.68 mg L− 1 were respectively obtained for similar exposure periods. At concentrations > 5 mg L− 1, mortality effects were significant, as were those relating to attachment and siphon extension (indicating filtration), both general indicators of health. Endocrine disruption effects were investigated after a prolonged exposure (112 d) to 5 and 500 μg L− 1 NP by measuring Vitellin (Vn)-like protein levels using the alkali-labile phosphate (ALP) assay and gel electrophoresis (GE). An increase in ALP levels was observed in both male and female mussels, although only marginal owing to a significant decrease in the mussels' health indicated by its condition, during the experiment. These levels, however, increased proportionally with NP concentration. Using solid phase thin-layer chromatography, we confirmed increased levels of the steroid cholesterol and evidence of NP uptake. Cholesterol levels in gonad tissue proved to be a more responsive biomarker of exposure to NP than levels of ALP. Further implications relating to the occurrence of endocrine disruption in the zebra mussel are discussed.

AB - Nonylphenol (NP) is commonly found in surface waters nearby municipal wastewater treatment plants and was shown to have endocrine disrupting effects in aquatic organisms. The purpose of this study was to investigate the toxicity and potential endocrine disrupting effects of NP on the freshwater zebra mussel (Dreissena polymorpha). Toxicity assessment yielded LC50 values of 3.68, 2.19 and 1.62 mg L− 1 after 15, 35 and 50 days of exposure, respectively. LC10 values of 1.6, 1.11 and 0.68 mg L− 1 were respectively obtained for similar exposure periods. At concentrations > 5 mg L− 1, mortality effects were significant, as were those relating to attachment and siphon extension (indicating filtration), both general indicators of health. Endocrine disruption effects were investigated after a prolonged exposure (112 d) to 5 and 500 μg L− 1 NP by measuring Vitellin (Vn)-like protein levels using the alkali-labile phosphate (ALP) assay and gel electrophoresis (GE). An increase in ALP levels was observed in both male and female mussels, although only marginal owing to a significant decrease in the mussels' health indicated by its condition, during the experiment. These levels, however, increased proportionally with NP concentration. Using solid phase thin-layer chromatography, we confirmed increased levels of the steroid cholesterol and evidence of NP uptake. Cholesterol levels in gonad tissue proved to be a more responsive biomarker of exposure to NP than levels of ALP. Further implications relating to the occurrence of endocrine disruption in the zebra mussel are discussed.

KW - Nonylphenol

KW - Zebra mussel

KW - Toxicity

KW - Endocrine disruption

U2 - 10.1016/j.cbpc.2005.11.004

DO - 10.1016/j.cbpc.2005.11.004

M3 - Article

VL - 142

SP - 118

EP - 127

JO - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology

JF - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology

SN - 1532-0456

IS - 1-2

ER -