European propolis is highly active against trypanosomatids including Crithidia fasciculata

Abdullah Alotaibi, Godwin U. Ebiloma, Roderick Williams, Samya Alenezi, Anne-Marie Donachie, Selome Guillaume, John O. Igoli, James Fearnley, Harry P. de Koning, David Watson

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Abstract

Extracts of 35 samples of European propolis were tested against wild type and resistant strains of the protozoal pathogens Trypanosoma brucei, Trypanosoma congolense and Leishmania mexicana. The extracts were also tested against Crithidia fasciculata a close relative of Crithidia mellificae, a parasite of bees. Crithidia, Trypanosoma and Leishmania are all members of the order Kinetoplastida. High levels of activity were obtained for all the samples with the levels of activity varying across the sample set. The highest levels of activity were found against L. mexicana. The propolis samples were profiled by using liquid chromatography with high resolution mass spectrometry (LC-MS) and principal components analysis (PCA) of the data obtained indicated there was a wide variation in the composition of the propolis samples. Orthogonal partial least squares (OPLS) associated a butyrate ester of pinobanksin with high activity against T. brucei whereas in the case of T. congolense high activity was associated with methyl ethers of chrysin and pinobanksin. In the case of C. fasciculata highest activity was associated with methyl ethers of galangin and pinobanksin. OPLS modelling of the activities against L. mexicana using the mass spectrometry produced a less successful model suggesting a wider range of active components.
Original languageEnglish
Article number11364
Number of pages11
JournalScientific Reports
Volume9
DOIs
Publication statusPublished - 6 Aug 2019

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Crithidia fasciculata
Trypanosomatidae
propolis
Leishmania mexicana
Crithidia
Trypanosoma congolense
Trypanosoma brucei
least squares
ethers
sampling
Kinetoplastida
mass spectrometry
Trypanosoma
Leishmania
extracts
butyrates
liquid chromatography
Apoidea
principal component analysis
esters

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Alotaibi, A., Ebiloma, G. U., Williams, R., Alenezi, S., Donachie, A-M., Guillaume, S., ... Watson, D. (2019). European propolis is highly active against trypanosomatids including Crithidia fasciculata. Scientific Reports, 9, [11364 ]. https://doi.org/10.1038/s41598-019-47840-y
Alotaibi, Abdullah ; Ebiloma, Godwin U. ; Williams, Roderick ; Alenezi, Samya ; Donachie, Anne-Marie ; Guillaume, Selome ; Igoli, John O. ; Fearnley, James ; de Koning, Harry P. ; Watson, David. / European propolis is highly active against trypanosomatids including Crithidia fasciculata. In: Scientific Reports. 2019 ; Vol. 9.
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Alotaibi, A, Ebiloma, GU, Williams, R, Alenezi, S, Donachie, A-M, Guillaume, S, Igoli, JO, Fearnley, J, de Koning, HP & Watson, D 2019, 'European propolis is highly active against trypanosomatids including Crithidia fasciculata', Scientific Reports, vol. 9, 11364 . https://doi.org/10.1038/s41598-019-47840-y

European propolis is highly active against trypanosomatids including Crithidia fasciculata. / Alotaibi, Abdullah; Ebiloma, Godwin U.; Williams, Roderick; Alenezi, Samya; Donachie, Anne-Marie; Guillaume, Selome; Igoli, John O.; Fearnley, James ; de Koning, Harry P.; Watson, David.

In: Scientific Reports, Vol. 9, 11364 , 06.08.2019.

Research output: Contribution to journalArticle

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AU - Alotaibi, Abdullah

AU - Ebiloma, Godwin U.

AU - Williams, Roderick

AU - Alenezi, Samya

AU - Donachie, Anne-Marie

AU - Guillaume, Selome

AU - Igoli, John O.

AU - Fearnley, James

AU - de Koning, Harry P.

AU - Watson, David

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AB - Extracts of 35 samples of European propolis were tested against wild type and resistant strains of the protozoal pathogens Trypanosoma brucei, Trypanosoma congolense and Leishmania mexicana. The extracts were also tested against Crithidia fasciculata a close relative of Crithidia mellificae, a parasite of bees. Crithidia, Trypanosoma and Leishmania are all members of the order Kinetoplastida. High levels of activity were obtained for all the samples with the levels of activity varying across the sample set. The highest levels of activity were found against L. mexicana. The propolis samples were profiled by using liquid chromatography with high resolution mass spectrometry (LC-MS) and principal components analysis (PCA) of the data obtained indicated there was a wide variation in the composition of the propolis samples. Orthogonal partial least squares (OPLS) associated a butyrate ester of pinobanksin with high activity against T. brucei whereas in the case of T. congolense high activity was associated with methyl ethers of chrysin and pinobanksin. In the case of C. fasciculata highest activity was associated with methyl ethers of galangin and pinobanksin. OPLS modelling of the activities against L. mexicana using the mass spectrometry produced a less successful model suggesting a wider range of active components.

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