A sensitive electrochemical assay method is described for secreted placental alkaline phosphatase (SPAP). The enzyme activity is measured using 2-naphthyl phosphate as the electrochemical substrate. The product of the reaction, 2-naphthol, is detected using Osteryoung square wave voltammetry (OSWV) with good sensitivity and precision using in-house screen printed carbon electrode assembly. The assay format is adapted to develop a cell based assay for herpes simplex virus (HSV).
|Number of pages||5|
|Publication status||Published - 15 May 2000|
- secreted alkaline phosphatase
- osteryoung square wave voltammetry
- cell based assay
- electrochemical detection of enzyme activity
- screen printed carbon electrodes