Electrochemical detection of secreted alkaline phosphatase: implications to cell based assays

Elizabeth Kelso, John McLean, Marco F. Cardosi

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

A sensitive electrochemical assay method is described for secreted placental alkaline phosphatase (SPAP). The enzyme activity is measured using 2-naphthyl phosphate as the electrochemical substrate. The product of the reaction, 2-naphthol, is detected using Osteryoung square wave voltammetry (OSWV) with good sensitivity and precision using in-house screen printed carbon electrode assembly. The assay format is adapted to develop a cell based assay for herpes simplex virus (HSV).
Original languageEnglish
Pages (from-to)490-494
Number of pages5
JournalElectroanalysis
Volume12
Issue number7
DOIs
Publication statusPublished - 15 May 2000

Keywords

  • secreted alkaline phosphatase
  • osteryoung square wave voltammetry
  • cell based assay
  • electrochemical detection of enzyme activity
  • screen printed carbon electrodes

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