Effects of subsequent high intensity interval training (HIIT) on l-arginine, dimethyarginines (adma;Sdma) and their ratios in ageing males

Nick Sculthorpe, John W.T. Ratcliffe, Peter Herbert, Dave Hullin, Liam Kilduff, Julien Baker, David M. Muggeridge, Fergal M. Grace

Research output: Contribution to journalMeeting Abstract


Advancing age, and sedentariness are primary risk factors for CV disease, however the interplay between aging, exercise and specific regulators of vascular function such as Asymmetric Dimethylarginine (ADMA) has not been extensively investigated. Recent evidence suggests that high intensity interval training (HIIT) can reduce a variety of CV risk factors PURPOSE: To examine the effects of a 7-week HIIT training programme delivered subsequent to an initial 6 week preconditioning plan on plasma ADMA, Symetric Dimethylarginine (SDMA) L-Arginine and their ratios in ageing males. METHODS: 39 apparently healthy aged males consisting of sedentary males (UTR) (n=22, age 62.4 + 5.4 yrs) and lifelong exercisers (TR) (n=17, 60.4 + 5.2 yrs). Blood was sampled from an antecubital forearm vein following 30-mins supine rest and treated appropriately until analysis by ELISA. Groups were assessed at baseline (A) after which UTR underwent 6 weeks of preconditioning whilst TR maintained their normal exercise regimen until reassessment (B). Subsequently, both groups underwent 7 weeks of HIIT training consisting of six 30’s bouts of cycle ergometry interspersed 3 minutes active recovery. HIIT training was carried out once every 5 days for the duration of the 7 weeks. Data were analysed using SPSS version 20.0; comparing group means utilising ANOVA followed by post-hoc Tukey test and students t-test. Statistical significance was set at p<0.05. Data are presented as mean + standard deviation (SD). RESULTS: ADMA was not different between groups and was unaffected by exercise intervention. SDMA. SDMA were reduced in UTR from A-B (0.38+0.14 - 0.44+0.15μmol.L-1) (p<0.05) and A-C (0.38+0.14 - 0.47+0.17μmol.L-1) (p<0.05) respectively. ADMA/SDMA ratio was reduced from A-B (2.0+0.6 - 1.7+0.5μmol.L-1) and B-C (1.7+0.5 - 1.5+0.5μmol.L-1) in UT group (both p<0.05) CONCLUSIONS: The findings of the present study indicate that preconditioning and HIIT exercise can affect plasma levels of SDMA and ADMA/SDMA ratios in previously sedentary older men and following HIIT in lifetime exercisers. The clinical significance of these alterations remains to be determined.BR>
Original languageEnglish
Pages (from-to)720-721
Number of pages2
JournalMedicine & Science in Sports & Exercise
Issue number5
Publication statusPublished - May 2014


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