Durum wheat stress tolerance induced by endophyte pantoea agglomerans with genes contributing to plant functions and secondary metabolite arsenal

Hafsa Cherif-Silini, Bathini Thissera, Ali Chenari Bouket, Nora Saadaoui, Allaoua Silini, Manal Eshelli, Faizah N. Alenezi, Armelle Vallat, Lenka Luptakova, Bilal Yahiaoui, Semcheddine Cherrad, Sebastien Vacher, Mostafa E. Rateb, Lassaad Belbahri*

*Corresponding author for this work

Research output: Contribution to journalArticle

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Abstract

In the arid region Bou-Saâda at the South of Algeria, durum wheat Triticum durum L. cv Waha production is severely threatened by abiotic stresses, mainly drought and salinity. Plant growth-promoting rhizobacteria (PGPR) hold promising prospects towards sustainable and environmentally-friendly agriculture. Using habitat-adapted symbiosis strategy, the PGPR Pantoea agglomerans strain Pa was recovered from wheat roots sampled in Bou-Saâda, conferred alleviation of salt stress in durum wheat plants and allowed considerable growth in this unhostile environment. Strain Pa showed growth up to 35 °C temperature, 5-10 pH range, and up to 30% polyethylene glycol (PEG), as well as 1 M salt concentration tolerance. Pa strain displayed pertinent plant growth promotion (PGP) features (direct and indirect) such as hormone auxin biosynthesis, production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia and phosphate solubilization. PGPR features were stable over wide salt concentrations (0-400 mM). Pa strain was also able to survive in seeds, in the non-sterile and sterile wheat rhizosphere, and was shown to have an endophytic life style. Phylogenomic analysis of strain Pa indicated that Pantoea genus suffers taxonomic imprecision which blurs species delimitation and may have impacted their practical use as biofertilizers. When applied to plants, strain Pa promoted considerable growth of wheat seedlings, high chlorophyll content, lower accumulation of proline, and favored K+ accumulation in the inoculated plants when compared to Na+ in control non-inoculated plants. Metabolomic profiling of strain Pa under one strain many compounds (OSMAC) conditions revealed a wide diversity of secondary metabolites (SM) with interesting salt stress alleviation and PGP activities. All these findings strongly promote the implementation of Pantoea agglomerans strain Pa as an efficient biofertilizer in wheat plants culture in arid and salinity-impacted regions.

Original languageEnglish
Article number3989
Number of pages36
JournalInternational Journal of Molecular Sciences
Volume20
Issue number16
DOIs
Publication statusPublished - 16 Aug 2019

Fingerprint

Pantoea
Arsenals
Endophytes
wheat
metabolites
Metabolites
genes
Triticum
Genes
Growth
Salts
salts
1-aminocyclopropane-1-carboxylate deaminase
promotion
salinity
Salinity
auxins
symbiosis
Algeria
Arid regions

Keywords

  • Abiotic stress
  • Antimicrobial activity
  • Durum wheat
  • PGPR
  • Pantoea agglomerans
  • Secondary metabolites
  • OSMAC
  • Metabolomics
  • Dereplication
  • Wheat microbiome

Cite this

Cherif-Silini, Hafsa ; Thissera, Bathini ; Bouket, Ali Chenari ; Saadaoui, Nora ; Silini, Allaoua ; Eshelli, Manal ; Alenezi, Faizah N. ; Vallat, Armelle ; Luptakova, Lenka ; Yahiaoui, Bilal ; Cherrad, Semcheddine ; Vacher, Sebastien ; Rateb, Mostafa E. ; Belbahri, Lassaad. / Durum wheat stress tolerance induced by endophyte pantoea agglomerans with genes contributing to plant functions and secondary metabolite arsenal. In: International Journal of Molecular Sciences. 2019 ; Vol. 20, No. 16.
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abstract = "In the arid region Bou-Sa{\^a}da at the South of Algeria, durum wheat Triticum durum L. cv Waha production is severely threatened by abiotic stresses, mainly drought and salinity. Plant growth-promoting rhizobacteria (PGPR) hold promising prospects towards sustainable and environmentally-friendly agriculture. Using habitat-adapted symbiosis strategy, the PGPR Pantoea agglomerans strain Pa was recovered from wheat roots sampled in Bou-Sa{\^a}da, conferred alleviation of salt stress in durum wheat plants and allowed considerable growth in this unhostile environment. Strain Pa showed growth up to 35 °C temperature, 5-10 pH range, and up to 30{\%} polyethylene glycol (PEG), as well as 1 M salt concentration tolerance. Pa strain displayed pertinent plant growth promotion (PGP) features (direct and indirect) such as hormone auxin biosynthesis, production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia and phosphate solubilization. PGPR features were stable over wide salt concentrations (0-400 mM). Pa strain was also able to survive in seeds, in the non-sterile and sterile wheat rhizosphere, and was shown to have an endophytic life style. Phylogenomic analysis of strain Pa indicated that Pantoea genus suffers taxonomic imprecision which blurs species delimitation and may have impacted their practical use as biofertilizers. When applied to plants, strain Pa promoted considerable growth of wheat seedlings, high chlorophyll content, lower accumulation of proline, and favored K+ accumulation in the inoculated plants when compared to Na+ in control non-inoculated plants. Metabolomic profiling of strain Pa under one strain many compounds (OSMAC) conditions revealed a wide diversity of secondary metabolites (SM) with interesting salt stress alleviation and PGP activities. All these findings strongly promote the implementation of Pantoea agglomerans strain Pa as an efficient biofertilizer in wheat plants culture in arid and salinity-impacted regions.",
keywords = "Abiotic stress, Antimicrobial activity, Durum wheat, PGPR, Pantoea agglomerans, Secondary metabolites, OSMAC, Metabolomics, Dereplication, Wheat microbiome",
author = "Hafsa Cherif-Silini and Bathini Thissera and Bouket, {Ali Chenari} and Nora Saadaoui and Allaoua Silini and Manal Eshelli and Alenezi, {Faizah N.} and Armelle Vallat and Lenka Luptakova and Bilal Yahiaoui and Semcheddine Cherrad and Sebastien Vacher and Rateb, {Mostafa E.} and Lassaad Belbahri",
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Cherif-Silini, H, Thissera, B, Bouket, AC, Saadaoui, N, Silini, A, Eshelli, M, Alenezi, FN, Vallat, A, Luptakova, L, Yahiaoui, B, Cherrad, S, Vacher, S, Rateb, ME & Belbahri, L 2019, 'Durum wheat stress tolerance induced by endophyte pantoea agglomerans with genes contributing to plant functions and secondary metabolite arsenal', International Journal of Molecular Sciences, vol. 20, no. 16, 3989. https://doi.org/10.3390/ijms20163989

Durum wheat stress tolerance induced by endophyte pantoea agglomerans with genes contributing to plant functions and secondary metabolite arsenal. / Cherif-Silini, Hafsa; Thissera, Bathini; Bouket, Ali Chenari; Saadaoui, Nora; Silini, Allaoua; Eshelli, Manal; Alenezi, Faizah N.; Vallat, Armelle; Luptakova, Lenka; Yahiaoui, Bilal; Cherrad, Semcheddine; Vacher, Sebastien; Rateb, Mostafa E.; Belbahri, Lassaad.

In: International Journal of Molecular Sciences, Vol. 20, No. 16, 3989, 16.08.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Durum wheat stress tolerance induced by endophyte pantoea agglomerans with genes contributing to plant functions and secondary metabolite arsenal

AU - Cherif-Silini, Hafsa

AU - Thissera, Bathini

AU - Bouket, Ali Chenari

AU - Saadaoui, Nora

AU - Silini, Allaoua

AU - Eshelli, Manal

AU - Alenezi, Faizah N.

AU - Vallat, Armelle

AU - Luptakova, Lenka

AU - Yahiaoui, Bilal

AU - Cherrad, Semcheddine

AU - Vacher, Sebastien

AU - Rateb, Mostafa E.

AU - Belbahri, Lassaad

PY - 2019/8/16

Y1 - 2019/8/16

N2 - In the arid region Bou-Saâda at the South of Algeria, durum wheat Triticum durum L. cv Waha production is severely threatened by abiotic stresses, mainly drought and salinity. Plant growth-promoting rhizobacteria (PGPR) hold promising prospects towards sustainable and environmentally-friendly agriculture. Using habitat-adapted symbiosis strategy, the PGPR Pantoea agglomerans strain Pa was recovered from wheat roots sampled in Bou-Saâda, conferred alleviation of salt stress in durum wheat plants and allowed considerable growth in this unhostile environment. Strain Pa showed growth up to 35 °C temperature, 5-10 pH range, and up to 30% polyethylene glycol (PEG), as well as 1 M salt concentration tolerance. Pa strain displayed pertinent plant growth promotion (PGP) features (direct and indirect) such as hormone auxin biosynthesis, production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia and phosphate solubilization. PGPR features were stable over wide salt concentrations (0-400 mM). Pa strain was also able to survive in seeds, in the non-sterile and sterile wheat rhizosphere, and was shown to have an endophytic life style. Phylogenomic analysis of strain Pa indicated that Pantoea genus suffers taxonomic imprecision which blurs species delimitation and may have impacted their practical use as biofertilizers. When applied to plants, strain Pa promoted considerable growth of wheat seedlings, high chlorophyll content, lower accumulation of proline, and favored K+ accumulation in the inoculated plants when compared to Na+ in control non-inoculated plants. Metabolomic profiling of strain Pa under one strain many compounds (OSMAC) conditions revealed a wide diversity of secondary metabolites (SM) with interesting salt stress alleviation and PGP activities. All these findings strongly promote the implementation of Pantoea agglomerans strain Pa as an efficient biofertilizer in wheat plants culture in arid and salinity-impacted regions.

AB - In the arid region Bou-Saâda at the South of Algeria, durum wheat Triticum durum L. cv Waha production is severely threatened by abiotic stresses, mainly drought and salinity. Plant growth-promoting rhizobacteria (PGPR) hold promising prospects towards sustainable and environmentally-friendly agriculture. Using habitat-adapted symbiosis strategy, the PGPR Pantoea agglomerans strain Pa was recovered from wheat roots sampled in Bou-Saâda, conferred alleviation of salt stress in durum wheat plants and allowed considerable growth in this unhostile environment. Strain Pa showed growth up to 35 °C temperature, 5-10 pH range, and up to 30% polyethylene glycol (PEG), as well as 1 M salt concentration tolerance. Pa strain displayed pertinent plant growth promotion (PGP) features (direct and indirect) such as hormone auxin biosynthesis, production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia and phosphate solubilization. PGPR features were stable over wide salt concentrations (0-400 mM). Pa strain was also able to survive in seeds, in the non-sterile and sterile wheat rhizosphere, and was shown to have an endophytic life style. Phylogenomic analysis of strain Pa indicated that Pantoea genus suffers taxonomic imprecision which blurs species delimitation and may have impacted their practical use as biofertilizers. When applied to plants, strain Pa promoted considerable growth of wheat seedlings, high chlorophyll content, lower accumulation of proline, and favored K+ accumulation in the inoculated plants when compared to Na+ in control non-inoculated plants. Metabolomic profiling of strain Pa under one strain many compounds (OSMAC) conditions revealed a wide diversity of secondary metabolites (SM) with interesting salt stress alleviation and PGP activities. All these findings strongly promote the implementation of Pantoea agglomerans strain Pa as an efficient biofertilizer in wheat plants culture in arid and salinity-impacted regions.

KW - Abiotic stress

KW - Antimicrobial activity

KW - Durum wheat

KW - PGPR

KW - Pantoea agglomerans

KW - Secondary metabolites

KW - OSMAC

KW - Metabolomics

KW - Dereplication

KW - Wheat microbiome

U2 - 10.3390/ijms20163989

DO - 10.3390/ijms20163989

M3 - Article

VL - 20

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 16

M1 - 3989

ER -