Development of an in vitro culture method for cells and tissues from the zebra mussel (Dreissena polymorpha)

Brian Quinn, Mark J. Costello, Germaine Dorange, James G. Wilson, Carmel Mothersill

Research output: Contribution to journalArticle

Abstract

Despite the successful transfer of mammalian in vitro techniques for use with fish and other vertebrates, little progress has been made in the area of invertebrate tissue culture. This paper describes the development of an in vitro technique for the culture of both cells in suspension and tissue explants from the gill, digestive gland and mantle of the zebra mussel (Dreissena polymorpha) and their successful maintenance in culture for up to 14 days. Cell suspensions from the gills and digestive gland were the most successful technique developed with viability > 80% maintained for up to 8 days in culture, suitable for use in short term toxicity tests. Tissue explants from the mantle were also maintained in culture for up to 14 days. This paper describes the challenges involved in the development of a novel in vitro culture technique for aquatic invertebrates.
Original languageEnglish
Pages (from-to)121-134
JournalCytotechnology
Volume59
Issue number2
DOIs
Publication statusPublished - Mar 2009
Externally publishedYes

Keywords

  • In vitro
  • Cell culture
  • Invertebrate
  • Zebra mussel
  • Mussel
  • Biomarker
  • Ecotoxicology
  • Pollution
  • Freshwater
  • Bioassay

Cite this

Quinn, Brian ; Costello, Mark J. ; Dorange, Germaine ; Wilson, James G. ; Mothersill, Carmel. / Development of an in vitro culture method for cells and tissues from the zebra mussel (Dreissena polymorpha). In: Cytotechnology. 2009 ; Vol. 59, No. 2. pp. 121-134.
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Development of an in vitro culture method for cells and tissues from the zebra mussel (Dreissena polymorpha). / Quinn, Brian; Costello, Mark J.; Dorange, Germaine; Wilson, James G.; Mothersill, Carmel.

In: Cytotechnology, Vol. 59, No. 2, 03.2009, p. 121-134.

Research output: Contribution to journalArticle

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AU - Quinn, Brian

AU - Costello, Mark J.

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KW - In vitro

KW - Cell culture

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KW - Mussel

KW - Biomarker

KW - Ecotoxicology

KW - Pollution

KW - Freshwater

KW - Bioassay

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